Restriction map for pF1KE1032
 (Linear) (Six-base) MAPSORT of: KIEE1032.seq  Check: 1513  from: 1  to: 4281



KIEE1032.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AccI GT'mk_AC

Cuts at:      0     627    4247    4281 
   Size:        627    3620      34

  Fragments arranged by size: 

                3620     627      34
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     944    2108    2357    2477    3095    4025    4281 
   Size:        944    1164     249     120     618     930     256

  Fragments arranged by size: 

                1164     944     930     618     256     249     120
 
AflIII A'CryG_T

Cuts at:      0     917    3183    4281 
   Size:        917    2266    1098

  Fragments arranged by size: 

                2266    1098     917
 
AleI CACnn'nnGTG

Cuts at:      0    3182    4281 
   Size:       3182    1099
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2946    4281 
   Size:       2946    1335
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1921    2113    2260    2854    2926    4281 
   Size:       1921     192     147     594      72    1355

  Fragments arranged by size: 

                1921    1355     594     192     147      72
 
ApoI r'AATT_y

Cuts at:      0     488     690     805     889     911    1242    1371    1656 
   Size:        488     202     115      84      22     331     129     285

Cuts at:   1656    1721    1865    1971    2676    3546    4170    4252    4281 
   Size:         65     144     106     705     870     624      82      29

  Fragments arranged by size: 

                 870     705     624     488     331     285     202     144
                 129     115     106      84      82      65      29      22
            
 
AseI AT'TA_AT

Cuts at:      0    1685    1745    2288    4003    4281 
   Size:       1685      60     543    1715     278

  Fragments arranged by size: 

                1715    1685     543     278      60
 
AvrII C'CTAG_G

Cuts at:      0     894    4281 
   Size:        894    3387
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     452     485    1277    1310    3662    3695    4281 
   Size:        452      33     792      33    2352      33     586

  Fragments arranged by size: 

                2352     792     586     452      33      33      33
 
BanII G_rGCy'C

Cuts at:      0     333    4281 
   Size:        333    3948
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1238    1463    1792    1880    2312    2465    3896    4281 
   Size:       1238     225     329      88     432     153    1431     385

  Fragments arranged by size: 

                1431    1238     432     385     329     225     153      88
            
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2953    4281 
   Size:       2953    1328
 
BclI T'GATC_A

Cuts at:      0    1506    4281 
   Size:       1506    2775
 
BfrBI ATG'CAT

Cuts at:      0    2200    4281 
   Size:       2200    2081
 
BglII A'GATC_T

Cuts at:      0    3102    4281 
   Size:       3102    1179
 
Bme1580I G_kGCm'C

Cuts at:      0    3803    4281 
   Size:       3803     478
 
BmgBI CAC'GTC

Cuts at:      0    3184    4281 
   Size:       3184    1097
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1061    2842    4214    4281 
   Size:       1061    1781    1372      67

  Fragments arranged by size: 

                1781    1372    1061      67
 
BmtI G_CTAG'C

Cuts at:      0     790    4281 
   Size:        790    3491
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1508    4158    4281 
   Size:       1508    2650     123

  Fragments arranged by size: 

                2650    1508     123
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     377     578    2051    2090    2792    3999    4281 
   Size:        377     201    1473      39     702    1207     282

  Fragments arranged by size: 

                1473    1207     702     377     282     201      39
 
Bpu10I CC'TnA_GC

Cuts at:      0     587     842    1952    2981    3816    4281 
   Size:        587     255    1110    1029     835     465

  Fragments arranged by size: 

                1110    1029     835     587     465     255
 
BsaHI Gr'CG_yC

Cuts at:      0    2062    4281 
   Size:       2062    2219
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     432     462    3195    3225    4281 
   Size:        432      30    2733      30    1056

  Fragments arranged by size: 

                2733    1056     432      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     437    2879    2882    3356    4040    4281 
   Size:        437    2442       3     474     684     241

  Fragments arranged by size: 

                2442     684     474     437     241       3
 
BseYI C'CCAG_C

Cuts at:      0     234     966    4281 
   Size:        234     732    3315

  Fragments arranged by size: 

                3315     732     234
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     422    2666    4281 
   Size:        422    2244    1615

  Fragments arranged by size: 

                2244    1615     422
 
BsiHKAI G_wGCw'C

Cuts at:      0     588    4096    4281 
   Size:        588    3508     185

  Fragments arranged by size: 

                3508     588     185
 
BsmI GAATG_Cn'

Cuts at:      0    3276    4281 
   Size:       3276    1005
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2217    4281 
   Size:       2217    2064
 
Bsp1286I G_dGCh'C

Cuts at:      0     333     588    3803    4096    4281 
   Size:        333     255    3215     293     185

  Fragments arranged by size: 

                3215     333     293     255     185
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      76    2309    2889    4281 
   Size:         76    2233     580    1392

  Fragments arranged by size: 

                2233    1392     580      76
 
BsrDI GCAATG_nn'

Cuts at:      0    2495    4264    4281 
   Size:       2495    1769      17

  Fragments arranged by size: 

                2495    1769      17
 
BsrFI r'CCGG_y

Cuts at:      0    3407    4281 
   Size:       3407     874
 
BsrGI T'GTAC_A

Cuts at:      0     121    1159    4281 
   Size:        121    1038    3122

  Fragments arranged by size: 

                3122    1038     121
 
BssSI C'ACGA_G

Cuts at:      0     746    4281 
   Size:        746    3535
 
BstYI r'GATC_y

Cuts at:      0     495     699    2785    3102    3684    4281 
   Size:        495     204    2086     317     582     597

  Fragments arranged by size: 

                2086     597     582     495     317     204
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    3533    4281 
   Size:       3533     748
 
BtsI GCAGTG_nn'

Cuts at:      0    3607    4281 
   Size:       3607     674
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3087    3122    4281 
   Size:       3087      35    1159

  Fragments arranged by size: 

                3087    1159      35
 
DraI TTT'AAA

Cuts at:      0     101     188     569    1284    1844    4281 
   Size:        101      87     381     715     560    2437

  Fragments arranged by size: 

                2437     715     560     381     101      87
 
DraIII CAC_nnn'GTG

Cuts at:      0    1399    2524    4281 
   Size:       1399    1125    1757

  Fragments arranged by size: 

                1757    1399    1125
 
EaeI y'GGCC_r

Cuts at:      0     483    4281 
   Size:        483    3798
 
EarI CTCTTCn'nnn_

Cuts at:      0     779    1579    1997    2105    3140    4281 
   Size:        779     800     418     108    1035    1141

  Fragments arranged by size: 

                1141    1035     800     779     418     108
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3699    4281 
   Size:       3699     582
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     377     578     944    2051    2090    2108    2357    2477 
   Size:        377     201     366    1107      39      18     249     120

Cuts at:   2477    2792    3095    3999    4025    4281 
   Size:        315     303     904      26     256

  Fragments arranged by size: 

                1107     904     377     366     315     303     256     249
                 201     120      39      26      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     455    2069    3005    3815    3873    4281 
   Size:        455    1614     936     810      58     408

  Fragments arranged by size: 

                1614     936     810     455     408      58
 
EcoO109I rG'GnC_Cy

Cuts at:      0    3864    4281 
   Size:       3864     417
 
EcoRI G'AATT_C

Cuts at:      0     889    1371    1971    4281 
   Size:        889     482     600    2310

  Fragments arranged by size: 

                2310     889     600     482
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     677    2591    4281 
   Size:        677    1914    1690

  Fragments arranged by size: 

                1914    1690     677
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1123    1155    1870    1902    1903    1935    1972    2004 
   Size:       1123      32     715      32       1      32      37      32

Cuts at:   2004    2407    2439    3173    3205    4281 
   Size:        403      32     734      32    1076

  Fragments arranged by size: 

                1123    1076     734     715     403      37      32      32
                  32      32      32      32       1
 
HincII GTy'rAC

Cuts at:      0     525    1048    2754    4281 
   Size:        525     523    1706    1527

  Fragments arranged by size: 

                1706    1527     525     523
 
HindIII A'AGCT_T

Cuts at:      0     373     563    4213    4281 
   Size:        373     190    3650      68

  Fragments arranged by size: 

                3650     373     190      68
 
MfeI C'AATT_G

Cuts at:      0     881    4281 
   Size:        881    3400
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2647    2841    3033    4281 
   Size:       2647     194     192    1248

  Fragments arranged by size: 

                2647    1248     194     192
 
MscI TGG'CCA

Cuts at:      0     485    4281 
   Size:        485    3796
 
MslI CAynn'nnrTG

Cuts at:      0     252    1844    2928    3182    3733    4003    4274    4281 
   Size:        252    1592    1084     254     551     270     271       7

  Fragments arranged by size: 

                1592    1084     551     271     270     254     252       7
            
 
MspA1I CmG'CkG

Cuts at:      0    1291    3163    3676    4281 
   Size:       1291    1872     513     605

  Fragments arranged by size: 

                1872    1291     605     513
 
NaeI GCC'GGC

Cuts at:      0    3409    4281 
   Size:       3409     872
 
NdeI CA'TA_TG

Cuts at:      0    2909    4281 
   Size:       2909    1372
 
NgoMIV G'CCGG_C

Cuts at:      0    3407    4281 
   Size:       3407     874
 
NheI G'CTAG_C

Cuts at:      0     786    4281 
   Size:        786    3495
 
NsiI A_TGCA'T

Cuts at:      0    2202    4281 
   Size:       2202    2079
 
NspI r_CATG'y

Cuts at:      0     297     607     921    4281 
   Size:        297     310     314    3360

  Fragments arranged by size: 

                3360     314     310     297
 
PciI A'CATG_T

Cuts at:      0     917    4281 
   Size:        917    3364
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2832    3246    4281 
   Size:       2832     414    1035

  Fragments arranged by size: 

                2832    1035     414
 
PstI C_TGCA'G

Cuts at:      0    2469    3949    4281 
   Size:       2469    1480     332

  Fragments arranged by size: 

                2469    1480     332
 
PvuII CAG'CTG

Cuts at:      0    1291    3163    3676    4281 
   Size:       1291    1872     513     605

  Fragments arranged by size: 

                1872    1291     605     513
 
SfcI C'TryA_G

Cuts at:      0    1445    2081    2384    2465    3599    3848    3945    4281 
   Size:       1445     636     303      81    1134     249      97     336

  Fragments arranged by size: 

                1445    1134     636     336     303     249      97      81
            
 
SpeI A'CTAG_T

Cuts at:      0     814    2355    4281 
   Size:        814    1541    1926

  Fragments arranged by size: 

                1926    1541     814
 
SphI G_CATG'C

Cuts at:      0     297    4281 
   Size:        297    3984
 
SspI AAT'ATT

Cuts at:      0     828    4281 
   Size:        828    3453
 
StuI AGG'CCT

Cuts at:      0    1279    4281 
   Size:       1279    3002
 
StyI C'CwwG_G

Cuts at:      0     307     894    2531    2728    2826    4281 
   Size:        307     587    1637     197      98    1455

  Fragments arranged by size: 

                1637    1455     587     307     197      98
 
SwaI ATTT'AAAT

Cuts at:      0    1844    4281 
   Size:       1844    2437
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    3315    4281 
   Size:       3315     966
 
TatI w'GTAC_w

Cuts at:      0     121    1159    2820    3186    3657    3898    4281 
   Size:        121    1038    1661     366     471     241     383

  Fragments arranged by size: 

                1661    1038     471     383     366     241     121
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2131    2163    3195    3227    4281 
   Size:       2131      32    1032      32    1054

  Fragments arranged by size: 

                2131    1054    1032      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1050    4281 
   Size:       1050    3231
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AleI     AlfI    AlwNI     ApoI     AseI
    AvrII     BaeI    BanII     BbsI    BciVI     BclI    BfrBI    BglII
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BsaHI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspMI    BsrDI    BsrFI    BsrGI    BssSI    BstYI    BtgZI     BtsI
    CspCI     DraI   DraIII     EaeI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI    Hin4I   HincII  HindIII     MfeI     MmeI
     MscI     MslI   MspA1I     NaeI     NdeI   NgoMIV     NheI     NsiI
     NspI     PciI    PflMI     PstI    PvuII     SfcI     SpeI     SphI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI    BamHI     BanI
     BbeI    BbvCI     BcgI     BglI     BlpI    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI    BsiEI    BsiWI    BspEI    BspHI    BsrBI   BssHII
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     ClaI
     DrdI     EagI  EcoICRI    EcoRV     FseI     FspI    FspAI    HaeII
     HpaI     KasI     KpnI     MluI     NarI     NcoI     NotI     NruI
     PacI     PasI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SrfI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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