Restriction map for
pF1KE1024
(Circular) (Six-base) MAPSORT of: pF1KE1024.seq Check: 7081 from: 1 to: 5537
pF1KE1024 5537 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1640 1640
Size: 5537
AccI GT'mk_AC
Cuts at: 2575 2575
Size: 5537
Acc65I G'GTAC_C
Cuts at: 2553 2553
Size: 5537
AclI AA'CG_TT
Cuts at: 5377 5377
Size: 5537
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1423 3362 3794 4147 4809 1423
Size: 1939 432 353 662 2151
Fragments arranged by size:
2151 1939 662 432 353
AflIII A'CryG_T
Cuts at: 1118 1733 2889 4689 4970 1118
Size: 615 1156 1800 281 1685
Fragments arranged by size:
1800 1685 1156 615 281
AleI CACnn'nnGTG
Cuts at: 262 373 262
Size: 111 5426
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 716 1502 716
Size: 786 4751
AlwNI CAG_nnn'CTG
Cuts at: 460 754 1130 1224 1389 2188 2842 4280 460
Size: 294 376 94 165 799 654 1438 1717
Fragments arranged by size:
1717 1438 799 654 376 294 165 94
ApaI G_GGCC'C
Cuts at: 203 2071 2136 2471 203
Size: 1868 65 335 3269
Fragments arranged by size:
3269 1868 335 65
ApaLI G'TGCA_C
Cuts at: 4375 4375
Size: 5537
ApoI r'AATT_y
Cuts at: 2541 4890 5146 2541
Size: 2349 256 2932
Fragments arranged by size:
2932 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5537
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5537
AvaI C'yCGr_G
Cuts at: 182 191 271 297 884 1053 1364 2024 2117
Size: 9 80 26 587 169 311 660 93
Cuts at: 2117 2557 182
Size: 440 3162
Fragments arranged by size:
3162 660 587 440 311 169 93 80
26 9
BamHI G'GATC_C
Cuts at: 1187 2562 1187
Size: 1375 4162
BanI G'GyrC_C
Cuts at: 123 154 595 1505 2553 2967 3215 3250 123
Size: 31 441 910 1048 414 248 35 2410
Fragments arranged by size:
2410 1048 910 441 414 248 35 31
BanII G_rGCy'C
Cuts at: 203 449 483 850 973 982 1072 1106 1287
Size: 246 34 367 123 9 90 34 181
Cuts at: 1287 1804 2071 2136 2471 2551 3581 203
Size: 517 267 65 335 80 1030 2159
Fragments arranged by size:
2159 1030 517 367 335 267 246 181
123 90 80 65 34 34 9
BbeI G_GCGC'C
Cuts at: 127 158 2971 3219 127
Size: 31 2813 248 2445
Fragments arranged by size:
2813 2445 248 31
BbsI GAAGACnn'nnnn_
Cuts at: 514 1892 514
Size: 1378 4159
BbvCI CC'TCA_GC
Cuts at: 2086 2201 2502 2086
Size: 115 301 5121
Fragments arranged by size:
5121 301 115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 298 332 1339 1373 298
Size: 34 1007 34 4462
Fragments arranged by size:
4462 1007 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3435 4491 5537 3435
Size: 1056 1046 3435
Fragments arranged by size:
3435 1056 1046
BclI T'GATC_A
Cuts at: 3057 3057
Size: 5537
BglI GCCn_nnn'nGGC
Cuts at: 152 163 247 493 888 1459 2007 2096 152
Size: 11 84 246 395 571 548 89 3593
Fragments arranged by size:
3593 571 548 395 246 89 84 11
BglII A'GATC_T
Cuts at: 1517 1583 3052 5089 1517
Size: 66 1469 2037 1965
Fragments arranged by size:
2037 1965 1469 66
BlpI GC'TnA_GC
Cuts at: 2649 2649
Size: 5537
Bme1580I G_kGCm'C
Cuts at: 121 203 600 2071 2136 2471 3162 3255 4379
Size: 82 397 1471 65 335 691 93 1124
Cuts at: 4379 121
Size: 1279
Fragments arranged by size:
1471 1279 1124 691 397 335 93 82
65
BmgBI CAC'GTC
Cuts at: 854 1086 854
Size: 232 5305
BmrI ACTGGGnnnn_n'
Cuts at: 1140 2749 2830 3165 5309 1140
Size: 1609 81 335 2144 1368
Fragments arranged by size:
2144 1609 1368 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3904 3904
Size: 5537
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 409 482 1267 1579 2061 2147 212
Size: 197 73 785 312 482 86 3602
Fragments arranged by size:
3602 785 482 312 197 86 73
Bpu10I CC'TnA_GC
Cuts at: 2086 2201 2502 2086
Size: 115 301 5121
Fragments arranged by size:
5121 301 115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 821 1945 2086 2414 2713 3592 4306 4604 821
Size: 1124 141 328 299 879 714 298 1754
Fragments arranged by size:
1754 1124 879 714 328 299 298 141
BsaI GGTCTCn'nnnn_
Cuts at: 304 1834 2260 5241 304
Size: 1530 426 2981 600
Fragments arranged by size:
2981 1530 600 426
BsaAI yAC'GTr
Cuts at: 2890 2890
Size: 5537
BsaBI GATnn'nnATC
Cuts at: 6 3051 5088 6
Size: 3045 2037 455
Fragments arranged by size:
3045 2037 455
BsaHI Gr'CG_yC
Cuts at: 124 155 1711 2968 3216 3918 5414 124
Size: 31 1556 1257 248 702 1496 247
Fragments arranged by size:
1556 1496 1257 702 248 247 31
BsaWI w'CCGG_w
Cuts at: 786 816 2726 3247 3925 4336 4483 4757 786
Size: 30 1910 521 678 411 147 274 1566
Fragments arranged by size:
1910 1566 678 521 411 274 147 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 303 333 798 828 832 862 904 934 1978
Size: 30 465 30 4 30 42 30 1044
Cuts at: 1978 2008 303
Size: 30 3832
Fragments arranged by size:
3832 1044 465 42 30 30 30 30
30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 529 1185 1285 2053 2064 2074 529
Size: 656 100 768 11 10 3992
Fragments arranged by size:
3992 768 656 100 11 10
BseYI C'CCAG_C
Cuts at: 333 345 810 1797 1868 1955 2170 2962 3720
Size: 12 465 987 71 87 215 792 758
Cuts at: 3720 4385 4793 333
Size: 665 408 1077
Fragments arranged by size:
1077 987 792 758 665 465 408 215
87 71 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 644 1312 1534 1571 644
Size: 668 222 37 4610
Fragments arranged by size:
4610 668 222 37
BsiEI CG_ry'CG
Cuts at: 104 308 3079 3125 4355 104
Size: 204 2771 46 1230 1286
Fragments arranged by size:
2771 1286 1230 204 46
BsiHKAI G_wGCw'C
Cuts at: 973 1072 1106 1661 2551 3329 3519 4379 973
Size: 99 34 555 890 778 190 860 2131
Fragments arranged by size:
2131 890 860 778 555 190 99 34
BsmI GAATG_Cn'
Cuts at: 1953 1953
Size: 5537
BsmBI CGTCTCn'nnnn_
Cuts at: 106 106
Size: 5537
Bsp1286I G_dGCh'C
Cuts at: 121 203 449 483 600 850 973 982 1072
Size: 82 246 34 117 250 123 9 90
Cuts at: 1072 1106 1287 1661 1804 2071 2136 2471 2551
Size: 34 181 374 143 267 65 335 80
Cuts at: 2551 3162 3255 3329 3519 3581 4379 121
Size: 611 93 74 190 62 798 1279
Fragments arranged by size:
1279 798 611 374 335 267 250 246
190 181 143 123 117 93 90 82
80 74 65 62 34 34 9
BspEI T'CCGG_A
Cuts at: 816 816
Size: 5537
BspHI T'CATG_A
Cuts at: 3969 3969
Size: 5537
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1334 1640 1871 2587 2801 3103 3484 1334
Size: 306 231 716 214 302 381 3387
Fragments arranged by size:
3387 716 381 306 302 231 214
BsrBI CCG'CTC
Cuts at: 50 1941 2289 2310 2331 2343 2406 3829 3883
Size: 1891 348 21 21 12 63 1423 54
Cuts at: 3883 5367 5532 50
Size: 1484 165 55
Fragments arranged by size:
1891 1484 1423 348 165 63 55 54
21 21 12
BsrDI GCAATG_nn'
Cuts at: 3449 3449
Size: 5537
BsrFI r'CCGG_y
Cuts at: 1421 1917 2045 3535 3716 1421
Size: 496 128 1490 181 3242
Fragments arranged by size:
3242 1490 496 181 128
BssSI C'ACGA_G
Cuts at: 1348 2035 3808 4516 1348
Size: 687 1773 708 2369
Fragments arranged by size:
2369 1773 708 687
BstAPI GCAn_nnn'nTGC
Cuts at: 667 1878 2914 667
Size: 1211 1036 3290
Fragments arranged by size:
3290 1211 1036
BstBI TT'CG_AA
Cuts at: 3898 3898
Size: 5537
BstEII G'GTnAC_C
Cuts at: 1905 1905
Size: 5537
BstXI CCAn_nnnn'nTGG
Cuts at: 1080 1305 1572 2488 1080
Size: 225 267 916 4129
Fragments arranged by size:
4129 916 267 225
BstYI r'GATC_y
Cuts at: 1 1187 1517 1583 2562 3029 3052 3387 3633
Size: 1186 330 66 979 467 23 335 246
Cuts at: 3633 3939 3951 4037 5089 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1186 1052 979 467 449 335 330 306
246 86 66 23 12
Bsu36I CC'TnA_GG
Cuts at: 648 747 648
Size: 99 5438
BtgI C'CryG_G
Cuts at: 107 161 449 974 1079 1457 1844 107
Size: 54 288 525 105 378 387 3800
Fragments arranged by size:
3800 525 387 378 288 105 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1609 3487 3668 3831 1609
Size: 1878 181 163 3315
Fragments arranged by size:
3315 1878 181 163
BtsI GCAGTG_nn'
Cuts at: 461 1879 2195 2780 461
Size: 1418 316 585 3218
Fragments arranged by size:
3218 1418 585 316
DraI TTT'AAA
Cuts at: 2536 2536
Size: 5537
DraIII CAC_nnn'GTG
Cuts at: 667 667
Size: 5537
DrdI GACnn_nn'nnGTC
Cuts at: 948 3243 4587 948
Size: 2295 1344 1898
Fragments arranged by size:
2295 1898 1344
EaeI y'GGCC_r
Cuts at: 239 305 485 3122 3296 3687 3714 5059 239
Size: 66 180 2637 174 391 27 1345 717
Fragments arranged by size:
2637 1345 717 391 180 174 66 27
EagI C'GGCC_G
Cuts at: 305 3122 305
Size: 2817 2720
EarI CTCTTCn'nnn_
Cuts at: 3560 3770 3560
Size: 210 5327
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 973 3745 4487 4633 5347 973
Size: 2772 742 146 714 1163
Fragments arranged by size:
2772 1163 742 714 146
EcoICRI GAG'CTC
Cuts at: 971 1070 1104 2549 971
Size: 99 34 1445 3959
Fragments arranged by size:
3959 1445 99 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 409 482 1267 1423 1579 2061 2147 3362
Size: 197 73 785 156 156 482 86 1215
Cuts at: 3362 3794 4147 4809 212
Size: 432 353 662 940
Fragments arranged by size:
1215 940 785 662 482 432 353 197
156 156 86 73
EcoNI CCTnn'n_nnAGG
Cuts at: 758 2180 758
Size: 1422 4115
EcoO109I rG'GnC_Cy
Cuts at: 223 294 548 560 572 751 763 865 894
Size: 71 254 12 12 179 12 102 29
Cuts at: 894 941 1293 1323 1386 1452 2021 2068 2132
Size: 47 352 30 63 66 569 47 64
Cuts at: 2132 2133 2185 2468 2476 2498 2676 223
Size: 1 52 283 8 22 178 3084
Fragments arranged by size:
3084 569 352 283 254 179 178 102
71 66 64 63 52 47 47 30
29 22 12 12 12 8 1
EcoRI G'AATT_C
Cuts at: 2541 2541
Size: 5537
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1047 1779 3556 1047
Size: 732 1777 3028
Fragments arranged by size:
3028 1777 732
FspI TGC'GCA
Cuts at: 1012 3318 1012
Size: 2306 3231
HaeII r_GCGC'y
Cuts at: 127 158 2046 2971 3219 4449 5220 127
Size: 31 1888 925 248 1230 771 444
Fragments arranged by size:
1888 1230 925 771 444 248 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 303 335 730 762 798 830 832 857 864
Size: 32 395 32 36 32 2 25 7
Cuts at: 864 889 955 987 2485 2517 3379 3409 3411
Size: 25 66 32 1498 32 862 30 2
Cuts at: 3411 3441 303
Size: 30 2399
Fragments arranged by size:
2399 1498 862 395 66 36 32 32
32 32 32 30 30 25 25 7
2 2
HincII GTy'rAC
Cuts at: 2576 2576
Size: 5537
HindIII A'AGCT_T
Cuts at: 2440 2440
Size: 5537
KasI G'GCGC_C
Cuts at: 123 154 2967 3215 123
Size: 31 2813 248 2445
Fragments arranged by size:
2813 2445 248 31
KpnI G_GTAC'C
Cuts at: 2557 2557
Size: 5537
MluI A'CGCG_T
Cuts at: 4970 4970
Size: 5537
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4296 4480 4296
Size: 184 5353
MscI TGG'CCA
Cuts at: 3298 3298
Size: 5537
MslI CAynn'nnrTG
Cuts at: 262 373 1078 1480 2486 3526 3653 262
Size: 111 705 402 1006 1040 127 2146
Fragments arranged by size:
2146 1040 1006 705 402 127 111
MspA1I CmG'CkG
Cuts at: 163 451 1098 1572 1617 1854 2125 2648 2962
Size: 288 647 474 45 237 271 523 314
Cuts at: 2962 3322 4106 4351 163
Size: 360 784 245 1349
Fragments arranged by size:
1349 784 647 523 474 360 314 288
271 245 237 45
NaeI GCC'GGC
Cuts at: 2047 3718 2047
Size: 1671 3866
NarI GG'CG_CC
Cuts at: 124 155 2968 3216 124
Size: 31 2813 248 2445
Fragments arranged by size:
2813 2445 248 31
NcoI C'CATG_G
Cuts at: 107 974 1844 107
Size: 867 870 3800
Fragments arranged by size:
3800 870 867
NgoMIV G'CCGG_C
Cuts at: 2045 3716 2045
Size: 1671 3866
NspI r_CATG'y
Cuts at: 1122 1485 1737 1836 1881 2590 3090 4693 1122
Size: 363 252 99 45 709 500 1603 1966
Fragments arranged by size:
1966 1603 709 500 363 252 99 45
PasI CC'CwG_GG
Cuts at: 1706 2064 2182 1706
Size: 358 118 5061
Fragments arranged by size:
5061 358 118
PciI A'CATG_T
Cuts at: 1118 1733 4689 1118
Size: 615 2956 1966
Fragments arranged by size:
2956 1966 615
PflMI CCAn_nnn'nTGG
Cuts at: 1214 1797 1214
Size: 583 4954
PfoI T'CCnGG_A
Cuts at: 219 219
Size: 5537
PmeI GTTT'AAAC
Cuts at: 2536 2536
Size: 5537
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3983 4015 3983
Size: 32 5505
PpuMI rG'GwC_Cy
Cuts at: 223 294 548 560 572 865 941 1293 1323
Size: 71 254 12 12 293 76 352 30
Cuts at: 1323 1386 2021 223
Size: 63 635 3739
Fragments arranged by size:
3739 635 352 293 254 76 71 63
30 12 12
PspOMI G'GGCC_C
Cuts at: 199 2067 2132 2467 199
Size: 1868 65 335 3269
Fragments arranged by size:
3269 1868 335 65
PspXI vC'TCGA_Gb
Cuts at: 884 1364 884
Size: 480 5057
PstI C_TGCA'G
Cuts at: 1994 2584 3269 1994
Size: 590 685 4262
Fragments arranged by size:
4262 685 590
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5537
PvuII CAG'CTG
Cuts at: 1572 1854 2125 2962 3322 1572
Size: 282 271 837 360 3787
Fragments arranged by size:
3787 837 360 282 271
RsrII CG'GwC_CG
Cuts at: 3732 3732
Size: 5537
SacI G_AGCT'C
Cuts at: 973 1072 1106 2551 973
Size: 99 34 1445 3959
Fragments arranged by size:
3959 1445 99 34
SacII CC_GC'GG
Cuts at: 164 452 164
Size: 288 5249
SalI G'TCGA_C
Cuts at: 2574 2574
Size: 5537
SanDI GG'GwC_CC
Cuts at: 294 548 560 1293 2021 294
Size: 254 12 733 728 3810
Fragments arranged by size:
3810 733 728 254 12
SapI GCTCTTCn'nnn_
Cuts at: 3560 3770 3560
Size: 210 5327
SbfI CC_TGCA'GG
Cuts at: 2584 2584
Size: 5537
ScaI AGT'ACT
Cuts at: 1051 5054 1051
Size: 4003 1534
SfcI C'TryA_G
Cuts at: 33 1545 1990 2580 3265 4233 4424 33
Size: 1512 445 590 685 968 191 1146
Fragments arranged by size:
1512 1146 968 685 590 445 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 247 247
Size: 5537
SfoI GGC'GCC
Cuts at: 125 156 2969 3217 125
Size: 31 2813 248 2445
Fragments arranged by size:
2813 2445 248 31
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5537
SgrAI Cr'CCGG_yG
Cuts at: 2045 2045
Size: 5537
SmaI CCC'GGG
Cuts at: 184 193 273 299 2026 2119 2559 184
Size: 9 80 26 1727 93 440 3162
Fragments arranged by size:
3162 1727 440 93 80 26 9
SmlI C'TyrA_G
Cuts at: 836 884 1364 1960 2101 2429 2692 3607 4321
Size: 48 480 596 141 328 263 915 714
Cuts at: 4321 4583 836
Size: 262 1790
Fragments arranged by size:
1790 915 714 596 480 328 263 262
141 48
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5537
SphI G_CATG'C
Cuts at: 1881 2590 3090 1881
Size: 709 500 4328
Fragments arranged by size:
4328 709 500
StuI AGG'CCT
Cuts at: 394 1408 394
Size: 1014 4523
StyI C'CwwG_G
Cuts at: 107 944 953 974 1035 1827 1844 2249 2671
Size: 837 9 21 61 792 17 405 422
Cuts at: 2671 3025 107
Size: 354 2619
Fragments arranged by size:
2619 837 792 422 405 354 61 21
17 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2244 3120 3253 3712 3921 2244
Size: 876 133 459 209 3860
Fragments arranged by size:
3860 876 459 209 133
TatI w'GTAC_w
Cuts at: 1049 5052 1049
Size: 4003 1534
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 215 247 626 658 1139 1171 1285 1317 5054
Size: 32 379 32 481 32 114 32 3737
Cuts at: 5054 5086 215
Size: 32 666
Fragments arranged by size:
3737 666 481 379 114 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3334 3334
Size: 5537
XbaI T'CTAG_A
Cuts at: 2568 2568
Size: 5537
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 858 3032 858
Size: 2174 3363
XhoI C'TCGA_G
Cuts at: 884 1364 884
Size: 480 5057
XmaI C'CCGG_G
Cuts at: 182 191 271 297 2024 2117 2557 182
Size: 9 80 26 1727 93 440 3162
Fragments arranged by size:
3162 1727 440 93 80 26 9
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI AscI AvrII
BaeI BfrBI BmtI BsiWI BsrGI BssHII BstZ17I ClaI
CspCI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PsrI
SexAI SnaBI SrfI SspI SwaI XmnI ZraI