Restriction map for pF1KE1022
##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AgeI        AocI        ApoI        AscI        
AseI        AsnI        Asp718I     AvrII       BbeI        
BbrPI       BcgI        BciVI       BfrI        BglI        
BglII       BlnI        BsaBI       BsiWI       Bsp1407I    
BspDI       BsrGI       BssHII      Bst1107I    BstEII      
BstPI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraIII      Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        HincII      HindII      
HindIII     HpaI        KasI        KpnI        MamI        
MfeI        MluI        MunI        NarI        NdeI        
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PstI        PvuI        
RsrII       SacI        SalI        SbfI        ScaI        
SfiI        SfoI        SgrAI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccIII        315  TCCGGA               T^CCGGA
AflIII        883  ACATGT               A^C[GA][CT]GT
AhdI         1986  GACATCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        799  GTGCAC               G^TGCAC
ApaI         2070  GGGCCC               GGGCC^C
ApaLI         799  GTGCAC               G^TGCAC
AspEI        1986  GACATCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        1235  TGCGCA               TGC^GCA
BalI         1964  TGGCCA               TGG^CCA
BamHI        1611  GGATCC               G^GATCC
BclI          728  TGATCA               T^GATCA
BfaI         1450  CTAG                 C^TAG
BlpI          297  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      297  GCTGAGC              GC^T[ACGT]AGC
BsaAI         609  TACGTA               [CT]AC^GT[GA]
BseAI         315  TCCGGA               T^CCGGA
BsgI         1345  CTGCAC               GTGCAG(16/14)
BsiEI        2093  CGGCCG               CG[GA][CT]^CG
BsmI         1644  GAATGC               GAATGC(1/-1)
BspEI         315  TCCGGA               T^CCGGA
BspHI        1415  TCATGA               T^CATGA
BspLU11I      883  ACATGT               A^CATGT
BsrDI         248  CATTGC               GCAATG(2/0)
BssSI         643  CACGAG               CACGAG(-5/-1)
BstAPI       2145  GCATGTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1641  TTCGAA               TT^CGAA
BstXI         440  CCATGGACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         297  GCTGAGC              GC^T[ACGT]AGC
DrdI         1317  GACGGGATCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         2093  CGGCCG               C^GGCCG
Eam1105I     1986  GACATCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2093  CGGCCG               C^GGCCG
Eco52I       2093  CGGCCG               C^GGCCG
EcoNI         447  CCTGGTCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          728  TGATCA               T^GATCA
FspI         1235  TGCGCA               TGC^GCA
KspI         1616  CCGCGG               CCGC^GG
MaeI         1450  CTAG                 C^TAG
MluNI        1964  TGGCCA               TGG^CCA
MroI          315  TCCGGA               T^CCGGA
MscI         1964  TGGCCA               TGG^CCA
MseI           10  TTAA                 T^TAA
MslI         1359  CATTGATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspV         1641  TTCGAA               TT^CGAA
PflMI        2162  CCAAGGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        1908  GACGGAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         1415  TCATGA               T^CATGA
SacII        1616  CCGCGG               CCGC^GG
SapI         2106  GAAGAGC              GCTCTTC(1/4)
SexAI         446  ACCTGGT              A^CC[AT]GGT
SfuI         1641  TTCGAA               TT^CGAA
SmlI         1531  CTCAAG               C^T[CT][GA]AG
SnaBI         609  TACGTA               TAC^GTA
StuI          434  AGGCCT               AGG^CCT
Tru9I          10  TTAA                 T^TAA
Van91I       2162  CCAAGGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1653  CCAGTACAAGTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

AccII        1617  CGCG                 CG^CG
             2091  CGCG                
AcyI          457  GACGCC               G[GA]^CG[CT]C
             1554  GGCGTC              
AfaI          612  GTAC                 GT^AC
             1656  GTAC                
AlwNI         715  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1077  CAGCCGCTG           
Aor51HI      1091  AGCGCT               AGC^GCT
             1935  AGCGCT              
Asp700I       489  GAAGCGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1036  GAAGATGTTC          
AspHI         799  GTGCAC               G[AT]GC[AT]^C
             1066  GTGCTC              
AspI          445  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1504  GACGATGTC           
AvaI         1194  CCCGGG               C^[CT]CG[GA]G
             2121  CCCGGG              
BsaHI         457  GACGCC               G[GA]^CG[CT]C
             1554  GGCGTC              
BsaI          160  GAGACC               GGTCTC(1/5)
              837  GAGACC              
BsaWI         315  TCCGGA               [AT]^CCGG[AT]
             2018  ACCGGA              
BsiHKAI       799  GTGCAC               G[AT]GC[AT]^C
             1066  GTGCTC              
BsmBI          25  CGTCTC               CGTCTC(1/5)
             1315  GAGACG              
BsoBI        1194  CCCGGG               C^[CT]CG[GA]G
             2121  CCCGGG              
BspMI        1048  ACCTGC               ACCTGC(4/8)
             1289  GCAGGT              
BsrBI        1171  CCGCTC               CCGCTC(-3/-3)
             1787  GAGCGG              
BstUI        1617  CGCG                 CG^CG
             2091  CGCG                
BstYI         206  AGATCC               [GA]^GATC[CT]
             1611  GGATCC              
Eco47III     1091  AGCGCT               AGC^GCT
             1935  AGCGCT              
HaeII        1091  AGCGCT               [GA]GCGC^[CT]
             1935  AGCGCT              
Hin1I         457  GACGCC               G[GA]^CG[CT]C
             1554  GGCGTC              
MflI          206  AGATCC               [GA]^GATC[CT]
             1611  GGATCC              
MvnI         1617  CGCG                 CG^CG
             2091  CGCG                
NaeI         1807  GCCGGC               GCC^GGC
             1865  GCCGGC              
NcoI          440  CCATGG               C^CATGG
             1550  CCATGG              
NgoMIV       1807  GCCGGC               G^CCGGC
             1865  GCCGGC              
NspI          883  ACATGT               [GA]CATG^[CT]
             2145  GCATGT              
PflFI         445  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1504  GACGATGTC           
RsaI          612  GTAC                 GT^AC
             1656  GTAC                
SfcI          741  CTACAG               C^T[GA][CT]AG
             1699  CTACAG              
SmaI         1194  CCCGGG               CCC^GGG
             2121  CCCGGG              
Tth111I       445  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1504  GACGATGTC           
XhoII         206  AGATCC               [GA]^GATC[CT]
             1611  GGATCC              
XmaCI        1194  CCCGGG               C^CCGGG
             2121  CCCGGG              
XmaI         1194  CCCGGG               C^CCGGG
             2121  CCCGGG              
XmnI          489  GAAGCGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1036  GAAGATGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BanI        
BanII       BbsI        BbvCI       BbvI        BcnI        
BmyI        BpmI        BpuAI       BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrFI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MnlI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PvuII       Sau3AI      Sau96I      ScrFI       SfaNI       
StyI        TaqI        TfiI        TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com