Restriction map for pF1KE1022
 (Linear) (Six-base) MAPSORT of: KIEE1022.seq  Check: 8368  from: 1  to: 2190



KIEE1022.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1057    1280    2190 
   Size:       1057     223     910

  Fragments arranged by size: 

                1057     910     223
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     670     742     815    1168    1298    1744    1864    1948 
   Size:        670      72      73     353     130     446     120      84

Cuts at:   1948    2190 
   Size:        242

  Fragments arranged by size: 

                 670     446     353     242     130     120      84      73
                  72
 
AfeI AGC'GCT

Cuts at:      0    1093    1937    2190 
   Size:       1093     844     253

  Fragments arranged by size: 

                1093     844     253
 
AflIII A'CryG_T

Cuts at:      0     883    2190 
   Size:        883    1307
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1991    2190 
   Size:       1991     199
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     575     607     903     935    1054    1086    2190 
   Size:        575      32     296      32     119      32    1104

  Fragments arranged by size: 

                1104     575     296     119      32      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     720    1082    2190 
   Size:        720     362    1108

  Fragments arranged by size: 

                1108     720     362
 
ApaI G_GGCC'C

Cuts at:      0    2074    2190 
   Size:       2074     116
 
ApaLI G'TGCA_C

Cuts at:      0     799    2190 
   Size:        799    1391
 
AvaI C'yCGr_G

Cuts at:      0    1194    2121    2190 
   Size:       1194     927      69

  Fragments arranged by size: 

                1194     927      69
 
BamHI G'GATC_C

Cuts at:      0    1611    2190 
   Size:       1611     579
 
BanI G'GyrC_C

Cuts at:      0     413    1097    1456    2015    2190 
   Size:        413     684     359     559     175

  Fragments arranged by size: 

                 684     559     413     359     175
 
BanII G_rGCy'C

Cuts at:      0     304    1976    2074    2190 
   Size:        304    1672      98     116

  Fragments arranged by size: 

                1672     304     116      98
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     821    1156    1286    1588    2190 
   Size:        821     335     130     302     602

  Fragments arranged by size: 

                 821     602     335     302     130
 
BbvCI CC'TCA_GC

Cuts at:      0     212    1026    1031    2190 
   Size:        212     814       5    1159

  Fragments arranged by size: 

                1159     814     212       5
 
BclI T'GATC_A

Cuts at:      0     728    2190 
   Size:        728    1462
 
BlpI GC'TnA_GC

Cuts at:      0     298    2190 
   Size:        298    1892
 
Bme1580I G_kGCm'C

Cuts at:      0     670     803     943    1459    2074    2190 
   Size:        670     133     140     516     615     116

  Fragments arranged by size: 

                 670     615     516     140     133     116
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     968    1647    2190 
   Size:        968     679     543

  Fragments arranged by size: 

                 968     679     543
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     453    1038    2190 
   Size:        453     585    1152

  Fragments arranged by size: 

                1152     585     453
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     835    1852    1873    1915    1921    1988    2028    2190 
   Size:        835    1017      21      42       6      67      40     162

  Fragments arranged by size: 

                1017     835     162      67      42      40      21       6
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     212    1026    1031    2190 
   Size:        212     814       5    1159

  Fragments arranged by size: 

                1159     814     212       5
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1516    2190 
   Size:       1516     674
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     154     831    2190 
   Size:        154     677    1359

  Fragments arranged by size: 

                1359     677     154
 
BsaAI yAC'GTr

Cuts at:      0     611    2190 
   Size:        611    1579
 
BsaHI Gr'CG_yC

Cuts at:      0     458    1555    2190 
   Size:        458    1097     635

  Fragments arranged by size: 

                1097     635     458
 
BsaWI w'CCGG_w

Cuts at:      0     315    2018    2190 
   Size:        315    1703     172

  Fragments arranged by size: 

                1703     315     172
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     575     605    1054    1084    1846    1876    2190 
   Size:        575      30     449      30     762      30     314

  Fragments arranged by size: 

                 762     575     449     314      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     176     397     400     445     770    1014    1726    1729 
   Size:        176     221       3      45     325     244     712       3

Cuts at:   1729    1849    1870    1873    2190 
   Size:        120      21       3     317

  Fragments arranged by size: 

                 712     325     317     244     221     176     120      45
                  21       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     841    1480    1757    2190 
   Size:        841     639     277     433

  Fragments arranged by size: 

                 841     639     433     277
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1330    2190 
   Size:       1330     860
 
BsiEI CG_ry'CG

Cuts at:      0    2096    2190 
   Size:       2096      94
 
BsiHKAI G_wGCw'C

Cuts at:      0     803    1070    2190 
   Size:        803     267    1120

  Fragments arranged by size: 

                1120     803     267
 
BsmI GAATG_Cn'

Cuts at:      0    1650    2190 
   Size:       1650     540
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      31    1309    2190 
   Size:         31    1278     881

  Fragments arranged by size: 

                1278     881      31
 
Bsp1286I G_dGCh'C

Cuts at:      0     304     670     803     943    1070    1459    1976    2074 
   Size:        304     366     133     140     127     389     517      98

Cuts at:   2074    2190 
   Size:        116

  Fragments arranged by size: 

                 517     389     366     304     140     133     127     116
                  98
 
BspEI T'CCGG_A

Cuts at:      0     315    2190 
   Size:        315    1875
 
BspHI T'CATG_A

Cuts at:      0    1415    2190 
   Size:       1415     775
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1057    1280    2190 
   Size:       1057     223     910

  Fragments arranged by size: 

                1057     910     223
 
BsrBI CCG'CTC

Cuts at:      0    1173    1789    2190 
   Size:       1173     616     401

  Fragments arranged by size: 

                1173     616     401
 
BsrDI GCAATG_nn'

Cuts at:      0     247    2190 
   Size:        247    1943
 
BsrFI r'CCGG_y

Cuts at:      0    1807    1865    2079    2190 
   Size:       1807      58     214     111

  Fragments arranged by size: 

                1807     214     111      58
 
BssSI C'ACGA_G

Cuts at:      0     643    2190 
   Size:        643    1547
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2151    2190 
   Size:       2151      39
 
BstBI TT'CG_AA

Cuts at:      0    1642    2190 
   Size:       1642     548
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     447    2190 
   Size:        447    1743
 
BstYI r'GATC_y

Cuts at:      0     206    1611    2190 
   Size:        206    1405     579

  Fragments arranged by size: 

                1405     579     206
 
BtgI C'CryG_G

Cuts at:      0     440     761    1176    1550    1616    2190 
   Size:        440     321     415     374      66     574

  Fragments arranged by size: 

                 574     440     415     374     321      66
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     364    2190 
   Size:        364    1826
 
BtsI GCAGTG_nn'

Cuts at:      0      92     721    2190 
   Size:         92     629    1469

  Fragments arranged by size: 

                1469     629      92
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1323    2190 
   Size:       1323     867
 
EaeI y'GGCC_r

Cuts at:      0     498    1242    1964    2093    2190 
   Size:        498     744     722     129      97

  Fragments arranged by size: 

                 744     722     498     129      97
 
EagI C'GGCC_G

Cuts at:      0    2093    2190 
   Size:       2093      97
 
EarI CTCTTCn'nnn_

Cuts at:      0     374     901    1720    2101    2190 
   Size:        374     527     819     381      89

  Fragments arranged by size: 

                 819     527     381     374      89
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1894    1961    2190 
   Size:       1894      67     229

  Fragments arranged by size: 

                1894     229      67
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     670     742     815     835    1168    1298    1744    1852 
   Size:        670      72      73      20     333     130     446     108

Cuts at:   1852    1864    1873    1915    1921    1948    1988    2028    2190 
   Size:         12       9      42       6      27      40      40     162

  Fragments arranged by size: 

                 670     446     333     162     130     108      73      72
                  42      40      40      27      20      12       9       6
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     451    2190 
   Size:        451    1739
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1737    2070    2071    2190 
   Size:       1737     333       1     119

  Fragments arranged by size: 

                1737     333     119       1
 
FspI TGC'GCA

Cuts at:      0    1237    2190 
   Size:       1237     953
 
HaeII r_GCGC'y

Cuts at:      0    1095    1939    2190 
   Size:       1095     844     251

  Fragments arranged by size: 

                1095     844     251
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     969    1001    1306    1338    1963    1977    1995    2009 
   Size:        969      32     305      32     625      14      18      14

Cuts at:   2009    2190 
   Size:        181

  Fragments arranged by size: 

                 969     625     305     181      32      32      18      14
                  14
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1097    1487    2190 
   Size:       1097     390     703

  Fragments arranged by size: 

                1097     703     390
 
MscI TGG'CCA

Cuts at:      0    1966    2190 
   Size:       1966     224
 
MslI CAynn'nnrTG

Cuts at:      0    1363    2190 
   Size:       1363     827
 
MspA1I CmG'CkG

Cuts at:      0     297    1030    1082    1484    1618    1851    2048    2190 
   Size:        297     733      52     402     134     233     197     142

  Fragments arranged by size: 

                 733     402     297     233     197     142     134      52
            
 
NaeI GCC'GGC

Cuts at:      0    1809    1867    2190 
   Size:       1809      58     323

  Fragments arranged by size: 

                1809     323      58
 
NcoI C'CATG_G

Cuts at:      0     440    1550    2190 
   Size:        440    1110     640

  Fragments arranged by size: 

                1110     640     440
 
NgoMIV G'CCGG_C

Cuts at:      0    1807    1865    2190 
   Size:       1807      58     325

  Fragments arranged by size: 

                1807     325      58
 
NspI r_CATG'y

Cuts at:      0     887    2149    2190 
   Size:        887    1262      41

  Fragments arranged by size: 

                1262     887      41
 
PasI CC'CwG_GG

Cuts at:      0    1956    2190 
   Size:       1956     234
 
PciI A'CATG_T

Cuts at:      0     883    2190 
   Size:        883    1307
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2168    2190 
   Size:       2168      22
 
PfoI T'CCnGG_A

Cuts at:      0      41    1829    2190 
   Size:         41    1788     361

  Fragments arranged by size: 

                1788     361      41
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      11      43     575     607    1054    1086    2190 
   Size:         11      32     532      32     447      32    1104

  Fragments arranged by size: 

                1104     532     447      32      32      32      11
 
PshAI GACnn'nnGTC

Cuts at:      0    1912    2190 
   Size:       1912     278
 
PspOMI G'GGCC_C

Cuts at:      0    2070    2190 
   Size:       2070     120
 
PvuII CAG'CTG

Cuts at:      0    1030    1851    2048    2190 
   Size:       1030     821     197     142

  Fragments arranged by size: 

                1030     821     197     142
 
SacII CC_GC'GG

Cuts at:      0    1619    2190 
   Size:       1619     571
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2101    2190 
   Size:       2101      89
 
SexAI A'CCwGG_T

Cuts at:      0     446    2190 
   Size:        446    1744
 
SfcI C'TryA_G

Cuts at:      0     741    1699    2190 
   Size:        741     958     491

  Fragments arranged by size: 

                 958     741     491
 
SmaI CCC'GGG

Cuts at:      0    1196    2123    2190 
   Size:       1196     927      67

  Fragments arranged by size: 

                1196     927      67
 
SmlI C'TyrA_G

Cuts at:      0    1531    2190 
   Size:       1531     659
 
SnaBI TAC'GTA

Cuts at:      0     611    2190 
   Size:        611    1579
 
StuI AGG'CCT

Cuts at:      0     436    2190 
   Size:        436    1754
 
StyI C'CwwG_G

Cuts at:      0     440     560     594     935    1550    2162    2190 
   Size:        440     120      34     341     615     612      28

  Fragments arranged by size: 

                 615     612     440     341     120      34      28
 
TatI w'GTAC_w

Cuts at:      0    1655    2190 
   Size:       1655     535
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     757     789    1846    1878    2190 
   Size:        757      32    1057      32     312

  Fragments arranged by size: 

                1057     757     312      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     448    1507    2190 
   Size:        448    1059     683

  Fragments arranged by size: 

                1059     683     448
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1660    2190 
   Size:       1660     530
 
XmaI C'CCGG_G

Cuts at:      0    1194    2121    2190 
   Size:       1194     927      69

  Fragments arranged by size: 

                1194     927      69
 
XmnI GAAnn'nnTTC

Cuts at:      0     493    1040    2190 
   Size:        493     547    1150

  Fragments arranged by size: 

                1150     547     493
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AhdI     AloI    AlwNI     ApaI
    ApaLI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BclI
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI     BtgI
    BtgZI     BtsI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoNI EcoO109I     FspI    HaeII    Hin4I     MmeI     MscI     MslI
   MspA1I     NaeI     NcoI   NgoMIV     NspI     PasI     PciI    PflMI
     PfoI     PpiI    PshAI   PspOMI    PvuII    SacII     SapI    SexAI
     SfcI     SmaI     SmlI    SnaBI     StuI     StyI     TatI     TstI
  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AleI     AlfI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI     BcgI
    BciVI    BfrBI     BglI    BglII    BmgBI     BmtI    BsaBI    BsiWI
    BsrGI   BssHII   BstEII  BstZ17I   Bsu36I     ClaI    CspCI     DraI
   DraIII  EcoICRI    EcoRI    EcoRV     FalI     FseI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NarI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PmeI     PmlI    PpuMI
     PsiI    PspXI     PsrI     PstI     PvuI    RsrII     SacI     SalI
    SanDI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     XbaI     XhoI     ZraI
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