Restriction map for pF1KB9259
 (Linear) (Six-base) MAPSORT of: KIBB9259.seq  Check: 3269  from: 1  to: 2937



KIBB9259.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1095    1902    1971    2937 
   Size:       1095     807      69     966

  Fragments arranged by size: 

                1095     966     807      69
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     197    1593    2937 
   Size:        197    1396    1344

  Fragments arranged by size: 

                1396    1344     197
 
AfeI AGC'GCT

Cuts at:      0    2017    2791    2937 
   Size:       2017     774     146

  Fragments arranged by size: 

                2017     774     146
 
AgeI A'CCGG_T

Cuts at:      0    1138    2937 
   Size:       1138    1799
 
AhdI GACnn_n'nnGTC

Cuts at:      0     976    2937 
   Size:        976    1961
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2385    2937 
   Size:       2385     552
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1410    2613    2937 
   Size:       1410    1203     324

  Fragments arranged by size: 

                1410    1203     324
 
ApaI G_GGCC'C

Cuts at:      0      79      93     185     292     449     593    1723    2159 
   Size:         79      14      92     107     157     144    1130     436

Cuts at:   2159    2166    2937 
   Size:          7     771

  Fragments arranged by size: 

                1130     771     436     157     144     107      92      79
                  14       7
 
AvaI C'yCGr_G

Cuts at:      0     147     448     639     912    1032    1805    2159    2476 
   Size:        147     301     191     273     120     773     354     317

Cuts at:   2476    2917    2937 
   Size:        441      20

  Fragments arranged by size: 

                 773     441     354     317     301     273     191     147
                 120      20
 
BamHI G'GATC_C

Cuts at:      0    1331    2577    2937 
   Size:       1331    1246     360

  Fragments arranged by size: 

                1331    1246     360
 
BanI G'GyrC_C

Cuts at:      0     337     512     564     755    1107    1791    1797    2435 
   Size:        337     175      52     191     352     684       6     638

Cuts at:   2435    2937 
   Size:        502

  Fragments arranged by size: 

                 684     638     502     352     337     191     175      52
                   6
 
BanII G_rGCy'C

Cuts at:      0      79      93     109     134     185     292     449     485 
   Size:         79      14      16      25      51     107     157      36

Cuts at:    485     499     593     646    1058    1348    1723    2147    2159 
   Size:         14      94      53     412     290     375     424      12

Cuts at:   2159    2166    2937 
   Size:          7     771

  Fragments arranged by size: 

                 771     424     412     375     290     157     107      94
                  79      53      51      36      25      16      14      14
                  12       7
 
BbeI G_GCGC'C

Cuts at:      0     568    1795    2937 
   Size:        568    1227    1142

  Fragments arranged by size: 

                1227    1142     568
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     314    2102    2172    2937 
   Size:        314    1788      70     765

  Fragments arranged by size: 

                1788     765     314      70
 
BbvCI CC'TCA_GC

Cuts at:      0     599    2937 
   Size:        599    2338
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1021    2937 
   Size:       1021    1916
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1522    2072    2086    2937 
   Size:       1522     550      14     851

  Fragments arranged by size: 

                1522     851     550      14
 
BglII A'GATC_T

Cuts at:      0    2906    2937 
   Size:       2906      31
 
BlpI GC'TnA_GC

Cuts at:      0    1387    2937 
   Size:       1387    1550
 
Bme1580I G_kGCm'C

Cuts at:      0      79      93     147     185     292     342     449     472 
   Size:         79      14      54      38     107      50     107      23

Cuts at:    472     593    1723    2159    2166    2600    2937 
   Size:        121    1130     436       7     434     337

  Fragments arranged by size: 

                1130     436     434     337     121     107     107      79
                  54      50      38      23      14       7
 
BmgBI CAC'GTC

Cuts at:      0      72    2937 
   Size:         72    2865
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1131    2937 
   Size:       1131    1806
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     138    1941    1983    2937 
   Size:        138    1803      42     954

  Fragments arranged by size: 

                1803     954     138      42
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      93     580     638     668     886    1272    1432    1465 
   Size:         93     487      58      30     218     386     160      33

Cuts at:   1465    1804    2074    2080    2446    2509    2692    2731    2937 
   Size:        339     270       6     366      63     183      39     206

  Fragments arranged by size: 

                 487     386     366     339     270     218     206     183
                 160      93      63      58      39      33      30       6
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     187     599    2937 
   Size:        187     412    2338

  Fragments arranged by size: 

                2338     412     187
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     915    1520    2647    2671    2937 
   Size:        915     605    1127      24     266

  Fragments arranged by size: 

                1127     915     605     266      24
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1063    2937 
   Size:       1063    1874
 
BsaAI yAC'GTr

Cuts at:      0    2338    2937 
   Size:       2338     599
 
BsaBI GATnn'nnATC

Cuts at:      0    2911    2937 
   Size:       2911      26
 
BsaHI Gr'CG_yC

Cuts at:      0     565    1463    1792    2937 
   Size:        565     898     329    1145

  Fragments arranged by size: 

                1145     898     565     329
 
BsaWI w'CCGG_w

Cuts at:      0    1065    1138    2937 
   Size:       1065      73    1799

  Fragments arranged by size: 

                1799    1065      73
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1405    1432    1435    1462    2327    2357    2419    2449 
   Size:       1405      27       3      27     865      30      62      30

Cuts at:   2449    2510    2540    2937 
   Size:         61      30     397

  Fragments arranged by size: 

                1405     865     397      62      61      30      30      30
                  27      27       3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     635     735    1225    1275    1549    1587    1873    2494 
   Size:        635     100     490      50     274      38     286     621

Cuts at:   2494    2737    2937 
   Size:        243     200

  Fragments arranged by size: 

                 635     621     490     286     274     243     200     100
                  50      38
 
BseYI C'CCAG_C

Cuts at:      0      39     102    2147    2937 
   Size:         39      63    2045     790

  Fragments arranged by size: 

                2045     790      63      39
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     550     927    1930    1999    2062    2937 
   Size:        550     377    1003      69      63     875

  Fragments arranged by size: 

                1003     875     550     377      69      63
 
BsiEI CG_ry'CG

Cuts at:      0      21    1715    1897    2307    2937 
   Size:         21    1694     182     410     630

  Fragments arranged by size: 

                1694     630     410     182      21
 
BsiHKAI G_wGCw'C

Cuts at:      0    1348    2432    2937 
   Size:       1348    1084     505

  Fragments arranged by size: 

                1348    1084     505
 
BsiWI C'GTAC_G

Cuts at:      0    2237    2937 
   Size:       2237     700
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1912    2937 
   Size:       1912    1025
 
Bsp1286I G_dGCh'C

Cuts at:      0      79      93     109     134     147     185     292     342 
   Size:         79      14      16      25      13      38     107      50

Cuts at:    342     449     472     485     499     593     646    1058    1348 
   Size:        107      23      13      14      94      53     412     290

Cuts at:   1348    1723    2147    2159    2166    2432    2600    2937 
   Size:        375     424      12       7     266     168     337

  Fragments arranged by size: 

                 424     412     375     337     290     266     168     107
                 107      94      79      53      50      38      25      23
                  16      14      14      13      13      12       7
 
BspEI T'CCGG_A

Cuts at:      0    1065    2937 
   Size:       1065    1872
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     107    1095    1481    1902    1971    2937 
   Size:        107     988     386     421      69     966

  Fragments arranged by size: 

                 988     966     421     386     107      69
 
BsrBI CCG'CTC

Cuts at:      0    1181    1217    2937 
   Size:       1181      36    1720

  Fragments arranged by size: 

                1720    1181      36
 
BsrFI r'CCGG_y

Cuts at:      0    1138    1525    1794    2247    2302    2778    2810    2937 
   Size:       1138     387     269     453      55     476      32     127

  Fragments arranged by size: 

                1138     476     453     387     269     127      55      32
            
 
BssHII G'CGCG_C

Cuts at:      0    1774    2827    2853    2937 
   Size:       1774    1053      26      84

  Fragments arranged by size: 

                1774    1053      84      26
 
BssSI C'ACGA_G

Cuts at:      0    1696    2937 
   Size:       1696    1241
 
BstEII G'GTnAC_C

Cuts at:      0       7    1129    2937 
   Size:          7    1122    1808

  Fragments arranged by size: 

                1808    1122       7
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     963    1972    2937 
   Size:        963    1009     965

  Fragments arranged by size: 

                1009     965     963
 
BstYI r'GATC_y

Cuts at:      0    1157    1331    2483    2495    2577    2906    2937 
   Size:       1157     174    1152      12      82     329      31

  Fragments arranged by size: 

                1157    1152     329     174      82      31      12
 
Bsu36I CC'TnA_GG

Cuts at:      0     218    2717    2937 
   Size:        218    2499     220

  Fragments arranged by size: 

                2499     220     218
 
BtgI C'CryG_G

Cuts at:      0      12    1199    1457    1766    2081    2804    2937 
   Size:         12    1187     258     309     315     723     133

  Fragments arranged by size: 

                1187     723     315     309     258     133      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1406    2937 
   Size:       1406    1531
 
BtsI GCAGTG_nn'

Cuts at:      0     470     495    2230    2508    2582    2937 
   Size:        470      25    1735     278      74     355

  Fragments arranged by size: 

                1735     470     355     278      74      25
 
EaeI y'GGCC_r

Cuts at:      0     282    1712    1894    2078    2087    2249    2304    2846 
   Size:        282    1430     182     184       9     162      55     542

Cuts at:   2846    2879    2937 
   Size:         33      58

  Fragments arranged by size: 

                1430     542     282     184     182     162      58      55
                  33       9
 
EagI C'GGCC_G

Cuts at:      0    1712    1894    2304    2937 
   Size:       1712     182     410     633

  Fragments arranged by size: 

                1712     633     410     182
 
EarI CTCTTCn'nnn_

Cuts at:      0     600    1982    2937 
   Size:        600    1382     955

  Fragments arranged by size: 

                1382     955     600
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1731    2473    2729    2857    2937 
   Size:       1731     742     256     128      80

  Fragments arranged by size: 

                1731     742     256     128      80
 
EcoICRI GAG'CTC

Cuts at:      0    1346    2937 
   Size:       1346    1591
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      93     197     580     638     668     886    1272    1432 
   Size:         93     104     383      58      30     218     386     160

Cuts at:   1432    1465    1593    1804    2074    2080    2446    2509    2692 
   Size:         33     128     211     270       6     366      63     183

Cuts at:   2692    2731    2937 
   Size:         39     206

  Fragments arranged by size: 

                 386     383     366     270     218     211     206     183
                 160     128     104      93      63      58      39      33
                  30       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     196     221     267    1167    2127    2937 
   Size:        196      25      46     900     960     810

  Fragments arranged by size: 

                 960     900     810     196      46      25
 
EcoO109I rG'GnC_Cy

Cuts at:      0      76      89      90     181     182     214     288     289 
   Size:         76      13       1      91       1      32      74       1

Cuts at:    289     445     652     833     881    1617    1647    1719    1720 
   Size:        156     207     181      48     736      30      72       1

Cuts at:   1720    2064    2103    2156    2163    2610    2628    2733    2937 
   Size:        344      39      53       7     447      18     105     204

  Fragments arranged by size: 

                 736     447     344     207     204     181     156     105
                  91      76      74      72      53      48      39      32
                  30      18      13       7       1       1       1       1
            
 
FseI GG_CCGG'CC

Cuts at:      0    2306    2937 
   Size:       2306     631
 
HaeII r_GCGC'y

Cuts at:      0     460     568     912    1734    1795    2019    2793    2894 
   Size:        460     108     344     822      61     224     774     101

Cuts at:   2894    2937 
   Size:         43

  Fragments arranged by size: 

                 822     774     460     344     224     108     101      61
                  43
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     190     222     958     962     990     994    1432    1464 
   Size:        190      32     736       4      28       4     438      32

Cuts at:   1464    2937 
   Size:       1473

  Fragments arranged by size: 

                1473     736     438     190      32      32      28       4
                   4
 
KasI G'GCGC_C

Cuts at:      0     564    1791    2937 
   Size:        564    1227    1146

  Fragments arranged by size: 

                1227    1146     564
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     902    2937 
   Size:        902    2035
 
MscI TGG'CCA

Cuts at:      0     284    2080    2089    2937 
   Size:        284    1796       9     848

  Fragments arranged by size: 

                1796     848     284       9
 
MslI CAynn'nnrTG

Cuts at:      0     949     955    1906    2398    2937 
   Size:        949       6     951     492     539

  Fragments arranged by size: 

                 951     949     539     492       6
 
MspA1I CmG'CkG

Cuts at:      0     232     932    1410    1576    1884    2699    2817    2937 
   Size:        232     700     478     166     308     815     118     120

  Fragments arranged by size: 

                 815     700     478     308     232     166     120     118
            
 
NaeI GCC'GGC

Cuts at:      0    1527    2249    2304    2937 
   Size:       1527     722      55     633

  Fragments arranged by size: 

                1527     722     633      55
 
NarI GG'CG_CC

Cuts at:      0     565    1792    2937 
   Size:        565    1227    1145

  Fragments arranged by size: 

                1227    1145     565
 
NcoI C'CATG_G

Cuts at:      0      12    1199    1766    2937 
   Size:         12    1187     567    1171

  Fragments arranged by size: 

                1187    1171     567      12
 
NdeI CA'TA_TG

Cuts at:      0      65    2937 
   Size:         65    2872
 
NgoMIV G'CCGG_C

Cuts at:      0    1525    2247    2302    2937 
   Size:       1525     722      55     635

  Fragments arranged by size: 

                1525     722     635      55
 
NspI r_CATG'y

Cuts at:      0     954    1494    2937 
   Size:        954     540    1443

  Fragments arranged by size: 

                1443     954     540
 
PasI CC'CwG_GG

Cuts at:      0     838    2296    2937 
   Size:        838    1458     641

  Fragments arranged by size: 

                1458     838     641
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     558     776    1410    1458    2445    2613    2699    2937 
   Size:        558     218     634      48     987     168      86     238

  Fragments arranged by size: 

                 987     634     558     238     218     168      86      48
            
 
PfoI T'CCnGG_A

Cuts at:      0     362    2317    2486    2606    2669    2708    2937 
   Size:        362    1955     169     120      63      39     229

  Fragments arranged by size: 

                1955     362     229     169     120      63      39
 
PpuMI rG'GwC_Cy

Cuts at:      0     881    2610    2628    2733    2937 
   Size:        881    1729      18     105     204

  Fragments arranged by size: 

                1729     881     204     105      18
 
PspOMI G'GGCC_C

Cuts at:      0      75      89     181     288     445     589    1719    2155 
   Size:         75      14      92     107     157     144    1130     436

Cuts at:   2155    2162    2937 
   Size:          7     775

  Fragments arranged by size: 

                1130     775     436     157     144     107      92      75
                  14       7
 
PstI C_TGCA'G

Cuts at:      0    1106    1738    2111    2937 
   Size:       1106     632     373     826

  Fragments arranged by size: 

                1106     826     632     373
 
PvuII CAG'CTG

Cuts at:      0    1410    2699    2937 
   Size:       1410    1289     238

  Fragments arranged by size: 

                1410    1289     238
 
SacI G_AGCT'C

Cuts at:      0    1348    2937 
   Size:       1348    1589
 
SexAI A'CCwGG_T

Cuts at:      0     515    2937 
   Size:        515    2422
 
SfcI C'TryA_G

Cuts at:      0    1102    1359    1734    2107    2635    2677    2937 
   Size:       1102     257     375     373     528      42     260

  Fragments arranged by size: 

                1102     528     375     373     260     257      42
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1522    2072    2086    2937 
   Size:       1522     550      14     851

  Fragments arranged by size: 

                1522     851     550      14
 
SfoI GGC'GCC

Cuts at:      0     566    1793    2937 
   Size:        566    1227    1144

  Fragments arranged by size: 

                1227    1144     566
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1138    1794    2937 
   Size:       1138     656    1143

  Fragments arranged by size: 

                1143    1138     656
 
SmaI CCC'GGG

Cuts at:      0     450     641    1034    1807    2161    2937 
   Size:        450     191     393     773     354     776

  Fragments arranged by size: 

                 776     773     450     393     354     191
 
SmlI C'TyrA_G

Cuts at:      0     894    1535    2662    2686    2937 
   Size:        894     641    1127      24     251

  Fragments arranged by size: 

                1127     894     641     251      24
 
SphI G_CATG'C

Cuts at:      0    1494    2937 
   Size:       1494    1443
 
SrfI GCCC'GGGC

Cuts at:      0     450     641    2937 
   Size:        450     191    2296

  Fragments arranged by size: 

                2296     450     191
 
StuI AGG'CCT

Cuts at:      0     118    2075    2937 
   Size:        118    1957     862

  Fragments arranged by size: 

                1957     862     118
 
StyI C'CwwG_G

Cuts at:      0      12     593    1073    1199    1766    2937 
   Size:         12     581     480     126     567    1171

  Fragments arranged by size: 

                1171     581     567     480     126      12
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      35     338     966    1759    2937 
   Size:         35     303     628     793    1178

  Fragments arranged by size: 

                1178     793     628     303      35
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     688     720    1316    1348    1405    1437    2327    2359 
   Size:        688      32     596      32      57      32     890      32

Cuts at:   2359    2419    2451    2510    2542    2937 
   Size:         60      32      59      32     395

  Fragments arranged by size: 

                 890     688     596     395      60      59      57      32
                  32      32      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1021    2937 
   Size:       1021    1916
 
XmaI C'CCGG_G

Cuts at:      0     448     639    1032    1805    2159    2937 
   Size:        448     191     393     773     354     778

  Fragments arranged by size: 

                 778     773     448     393     354     191
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AgeI     AhdI     AlfI    AlwNI     ApaI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I
    BspEI    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstEII    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FseI    HaeII    Hin4I
     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NspI     PasI    PflMI     PfoI    PpuMI   PspOMI
     PstI    PvuII     SacI    SexAI     SfcI     SfiI     SfoI    SgrAI
     SmaI     SmlI     SphI     SrfI     StuI     StyI    TaqII     TstI
     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AleI     AloI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BcgI
     BclI    BfrBI     BmtI     BsmI    BspHI    BsrDI    BsrGI   BstAPI
    BstBI  BstZ17I     ClaI    CspCI     DraI   DraIII     DrdI    EcoRI
    EcoRV     FalI     FspI    FspAI   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     NheI     NotI     NruI     NsiI     PacI     PciI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI     SgfI
    SnaBI     SpeI     SspI     SwaI     TatI  Tth111I     XbaI     XhoI
     XmnI     ZraI
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