Restriction map for pF1KB6014
##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AgeI        AocI        Aor51HI     AscI        AseI        
AsnI        Asp718I     AviII       AvrII       BanI        
BbeI        BbvCI       BcgI        BciVI       BclI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaBI       BsiWI       BsmBI       BsmI        BspDI       
BspHI       BspLU11I    Bst1107I    BstAPI      BstBI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DrdI        Eco47III    Eco81I      EcoNI       
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HpaI        KasI        KpnI        MamI        
MfeI        MluI        MunI        NarI        NdeI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PinAI       PmeI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SalI        SapI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccIII       1596  TCCGGA               T^CCGGA
AcyI          181  GACGCC               G[GA]^CG[CT]C
AflIII       1706  ACACGT               A^C[GA][CT]GT
AhdI          976  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1838  GTGCAC               G^TGCAC
AlwNI        1900  CAGAGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        1838  GTGCAC               G^TGCAC
Asp700I      1299  GAAACTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         976  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1186  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI        1657  CACGTG               CAC^GTG
BsaHI         181  GACGCC               G[GA]^CG[CT]C
BsaI         1805  GAGACC               GGTCTC(1/5)
BseAI        1596  TCCGGA               T^CCGGA
BsgI          250  GTGCAG               GTGCAG(16/14)
Bsp1407I      965  TGTACA               T^GTACA
BspEI        1596  TCCGGA               T^CCGGA
BspMI          51  ACCTGC               ACCTGC(4/8)
BsrBI          44  GAGCGG               CCGCTC(-3/-3)
BsrDI         863  CATTGC               GCAATG(2/0)
BsrFI         548  GCCGGC               [GA]^CCGG[CT]
BsrGI         965  TGTACA               T^GTACA
BssHII         25  GCGCGC               G^CGCGC
BssSI         247  CTCGTG               CACGAG(-5/-1)
Cfr10I        548  GCCGGC               [GA]^CCGG[CT]
DraIII       1832  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      976  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII          68  GGCGCT               [GA]GCGC^[CT]
Hin1I         181  GACGCC               G[GA]^CG[CT]C
HindIII      1579  AAGCTT               A^AGCTT
MroI         1596  TCCGGA               T^CCGGA
NaeI          548  GCCGGC               GCC^GGC
NgoMIV        548  GCCGGC               G^CCGGC
NheI          117  GCTAGC               G^CTAGC
PflFI        1186  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1657  CACGTG               CAC^GTG
PmlI         1657  CACGTG               CAC^GTG
PshAI        1978  GACATGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII        1641  CAGCTG               CAG^CTG
SmlI         1057  CTCAAG               C^T[CT][GA]AG
SspBI         965  TGTACA               T^GTACA
SspI         1218  AATATT               AAT^ATT
StuI         1852  AGGCCT               AGG^CCT
Tth111I      1186  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI         1299  GAAACTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

BamHI        1163  GGATCC               G^GATCC
             1563  GGATCC              
BbsI         1050  GAAGAC               GAAGAC(2/6)
             1366  GAAGAC              
BpuAI        1050  GAAGAC               GAAGAC(2/6)
             1366  GAAGAC              
BsaWI         425  TCCGGT               [AT]^CCGG[AT]
             1596  TCCGGA              
BseRI        1398  GAGGAG               GAGGAG(10/8)
             1768  GAGGAG              
BstEII        711  GGTGACC              G^GT[ACGT]ACC
             1279  GGTTACC             
BstPI         711  GGTGACC              G^GT[ACGT]ACC
             1279  GGTTACC             
BstYI        1163  GGATCC               [GA]^GATC[CT]
             1563  GGATCC              
DraI          433  TTTAAA               TTT^AAA
              472  TTTAAA              
EarI          370  GAAGAG               CTCTTC(1/4)
             1765  GAAGAG              
EcoO65I       711  GGTGACC              G^GT[ACGT]ACC
             1279  GGTTACC             
HincII       1192  GTCAAC               GT[CT]^[GA]AC
             1960  GTTGAC              
HindII       1192  GTCAAC               GT[CT]^[GA]AC
             1960  GTTGAC              
Ksp632I       370  GAAGAG               CTCTTC(1/4)
             1765  GAAGAG              
KspI           82  CCGCGG               CCGC^GG
              100  CCGCGG              
MflI         1163  GGATCC               [GA]^GATC[CT]
             1563  GGATCC              
NcoI         1469  CCATGG               C^CATGG
             1800  CCATGG              
PpuMI          48  GGGACCT              [GA]G^G[AT]CC[CT]
              477  AGGACCT             
SacII          82  CCGCGG               CCGC^GG
              100  CCGCGG              
SexAI        1187  ACCTGGT              A^CC[AT]GGT
             1833  ACCTGGT             
SmaI           33  CCCGGG               CCC^GGG
             1915  CCCGGG              
TaqI          958  TCGA                 T^CGA
             1330  TCGA                
Tsp45I        712  GTGAC                ^GT[GC]AC
             1069  GTGAC               
TthHB8I       958  TCGA                 T^CGA
             1330  TCGA                
XhoII        1163  GGATCC               [GA]^GATC[CT]
             1563  GGATCC              
XmaCI          33  CCCGGG               C^CCGGG
             1915  CCCGGG              
XmaI           33  CCCGGG               C^CCGGG
             1915  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   96 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApaI        ApoI        AspHI       AvaI        
AvaII       BalI        BanII       BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BsaAI       
BsaJI       BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EagI        EclXI       Eco52I      
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PflMI       
PleI        PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TfiI        
Tru9I       TseI        Tsp509I     TspRI       Van91I      
XcmI        
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