Restriction map for pF1KB6014
 (Linear) (Six-base) MAPSORT of: KIBB6014.seq  Check: 8881  from: 1  to: 2094



KIBB6014.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     749    1069    1567    2094 
   Size:        749     320     498     527

  Fragments arranged by size: 

                 749     527     498     320
 
AflIII A'CryG_T

Cuts at:      0    1706    2094 
   Size:       1706     388
 
AhdI GACnn_n'nnGTC

Cuts at:      0     981    2094 
   Size:        981    1113
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1905    2094 
   Size:       1905     189
 
ApaI G_GGCC'C

Cuts at:      0     309    1849    1916    1923    2094 
   Size:        309    1540      67       7     171

  Fragments arranged by size: 

                1540     309     171      67       7
 
ApaLI G'TGCA_C

Cuts at:      0    1838    2094 
   Size:       1838     256
 
ApoI r'AATT_y

Cuts at:      0     468     921     946    2094 
   Size:        468     453      25    1148

  Fragments arranged by size: 

                1148     468     453      25
 
AvaI C'yCGr_G

Cuts at:      0      33     716    1915    1954    2094 
   Size:         33     683    1199      39     140

  Fragments arranged by size: 

                1199     683     140      39      33
 
BamHI G'GATC_C

Cuts at:      0    1163    1563    2094 
   Size:       1163     400     531

  Fragments arranged by size: 

                1163     531     400
 
BanII G_rGCy'C

Cuts at:      0      66     309    1849    1916    1923    2094 
   Size:         66     243    1540      67       7     171

  Fragments arranged by size: 

                1540     243     171      67      66       7
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1057    1373    2094 
   Size:       1057     316     721

  Fragments arranged by size: 

                1057     721     316
 
BglI GCCn_nnn'nGGC

Cuts at:      0      16     300    1547    2094 
   Size:         16     284    1247     547

  Fragments arranged by size: 

                1247     547     284      16
 
Bme1580I G_kGCm'C

Cuts at:      0     309    1842    1849    1916    1923    2094 
   Size:        309    1533       7      67       7     171

  Fragments arranged by size: 

                1533     309     171      67       7       7
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     339     581    1852    2094 
   Size:        339     242    1271     242

  Fragments arranged by size: 

                1271     339     242     242
 
BmtI G_CTAG'C

Cuts at:      0     121    2094 
   Size:        121    1973
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     482     766    1000    1882    1953    2094 
   Size:        482     284     234     882      71     141

  Fragments arranged by size: 

                 882     482     284     234     141      71
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1042    2094 
   Size:       1042    1052
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1799    2094 
   Size:       1799     295
 
BsaAI yAC'GTr

Cuts at:      0     630    1659    1709    2004    2094 
   Size:        630    1029      50     295      90

  Fragments arranged by size: 

                1029     630     295      90      50
 
BsaHI Gr'CG_yC

Cuts at:      0     182    2094 
   Size:        182    1912
 
BsaWI w'CCGG_w

Cuts at:      0     425    1596    2094 
   Size:        425    1171     498

  Fragments arranged by size: 

                1171     498     425
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     983    1013    2094 
   Size:        983      30    1081

  Fragments arranged by size: 

                1081     983      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1413    1783    2094 
   Size:       1413     370     311

  Fragments arranged by size: 

                1413     370     311
 
BseYI C'CCAG_C

Cuts at:      0     313    1608    1643    2094 
   Size:        313    1295      35     451

  Fragments arranged by size: 

                1295     451     313      35
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271    2094 
   Size:        271    1823
 
BsiEI CG_ry'CG

Cuts at:      0      11     239    1102    1539    2090    2094 
   Size:         11     228     863     437     551       4

  Fragments arranged by size: 

                 863     551     437     228      11       4
 
BsiHKAI G_wGCw'C

Cuts at:      0     723    1445    1664    1842    2094 
   Size:        723     722     219     178     252

  Fragments arranged by size: 

                 723     722     252     219     178
 
Bsp1286I G_dGCh'C

Cuts at:      0      66     309     723    1445    1664    1842    1849    1916 
   Size:         66     243     414     722     219     178       7      67

Cuts at:   1916    1923    2094 
   Size:          7     171

  Fragments arranged by size: 

                 722     414     243     219     178     171      67      66
                   7       7
 
BspEI T'CCGG_A

Cuts at:      0    1596    2094 
   Size:       1596     498
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      60    2094 
   Size:         60    2034
 
BsrBI CCG'CTC

Cuts at:      0      46    2094 
   Size:         46    2048
 
BsrDI GCAATG_nn'

Cuts at:      0     862    2094 
   Size:        862    1232
 
BsrFI r'CCGG_y

Cuts at:      0     548    2094 
   Size:        548    1546
 
BsrGI T'GTAC_A

Cuts at:      0     965    2094 
   Size:        965    1129
 
BssHII G'CGCG_C

Cuts at:      0      25    2094 
   Size:         25    2069
 
BssSI C'ACGA_G

Cuts at:      0     247    2094 
   Size:        247    1847
 
BstEII G'GTnAC_C

Cuts at:      0     711    1279    2094 
   Size:        711     568     815

  Fragments arranged by size: 

                 815     711     568
 
BstYI r'GATC_y

Cuts at:      0    1163    1563    2094 
   Size:       1163     400     531

  Fragments arranged by size: 

                1163     531     400
 
BtgI C'CryG_G

Cuts at:      0      82     100     615    1469    1800    2094 
   Size:         82      18     515     854     331     294

  Fragments arranged by size: 

                 854     515     331     294      82      18
 
BtsI GCAGTG_nn'

Cuts at:      0     276    2094 
   Size:        276    1818
 
DraI TTT'AAA

Cuts at:      0     435     474    2094 
   Size:        435      39    1620

  Fragments arranged by size: 

                1620     435      39
 
DraIII CAC_nnn'GTG

Cuts at:      0    1837    2094 
   Size:       1837     257
 
EaeI y'GGCC_r

Cuts at:      0       8     236     273    1034    1079    1099    1142    1536 
   Size:          8     228      37     761      45      20      43     394

Cuts at:   1536    1637    1686    1751    2087    2094 
   Size:        101      49      65     336       7

  Fragments arranged by size: 

                 761     394     336     228     101      65      49      45
                  43      37      20       8       7
 
EagI C'GGCC_G

Cuts at:      0       8     236    1099    1536    2087    2094 
   Size:          8     228     863     437     551       7

  Fragments arranged by size: 

                 863     551     437     228       8       7
 
EarI CTCTTCn'nnn_

Cuts at:      0     365    1760    2094 
   Size:        365    1395     334

  Fragments arranged by size: 

                1395     365     334
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     482     749     766    1000    1069    1567    1882    1953 
   Size:        482     267      17     234      69     498     315      71

Cuts at:   1953    2094 
   Size:        141

  Fragments arranged by size: 

                 498     482     315     267     234     141      71      69
                  17
 
EcoO109I rG'GnC_Cy

Cuts at:      0      49     306     478    1632    1846    1919    2094 
   Size:         49     257     172    1154     214      73     175

  Fragments arranged by size: 

                1154     257     214     175     172      73      49
 
HaeII r_GCGC'y

Cuts at:      0      72    2094 
   Size:         72    2022
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     967     999    1548    1580    1812    1844    1860    1892 
   Size:        967      32     549      32     232      32      16      32

Cuts at:   1892    2094 
   Size:        202

  Fragments arranged by size: 

                 967     549     232     202      32      32      32      32
                  16
 
HincII GTy'rAC

Cuts at:      0    1194    1962    2094 
   Size:       1194     768     132

  Fragments arranged by size: 

                1194     768     132
 
HindIII A'AGCT_T

Cuts at:      0    1579    2094 
   Size:       1579     515
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1100    1227    2094 
   Size:       1100     127     867

  Fragments arranged by size: 

                1100     867     127
 
MscI TGG'CCA

Cuts at:      0     275    1081    1144    1639    1753    2094 
   Size:        275     806      63     495     114     341

  Fragments arranged by size: 

                 806     495     341     275     114      63
 
MslI CAynn'nnrTG

Cuts at:      0     709    1340    1556    2029    2094 
   Size:        709     631     216     473      65

  Fragments arranged by size: 

                 709     631     473     216      65
 
MspA1I CmG'CkG

Cuts at:      0      84     102     203    1643    2094 
   Size:         84      18     101    1440     451

  Fragments arranged by size: 

                1440     451     101      84      18
 
NaeI GCC'GGC

Cuts at:      0     550    2094 
   Size:        550    1544
 
NcoI C'CATG_G

Cuts at:      0    1469    1800    2094 
   Size:       1469     331     294

  Fragments arranged by size: 

                1469     331     294
 
NgoMIV G'CCGG_C

Cuts at:      0     548    2094 
   Size:        548    1546
 
NheI G'CTAG_C

Cuts at:      0     117    2094 
   Size:        117    1977
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     630    1505    1905    2063    2094 
   Size:        630     875     400     158      31

  Fragments arranged by size: 

                 875     630     400     158      31
 
PmlI CAC'GTG

Cuts at:      0    1659    2094 
   Size:       1659     435
 
PpuMI rG'GwC_Cy

Cuts at:      0      49     478    2094 
   Size:         49     429    1616

  Fragments arranged by size: 

                1616     429      49
 
PshAI GACnn'nnGTC

Cuts at:      0    1982    2094 
   Size:       1982     112
 
PspOMI G'GGCC_C

Cuts at:      0     305    1845    1912    1919    2094 
   Size:        305    1540      67       7     175

  Fragments arranged by size: 

                1540     305     175      67       7
 
PstI C_TGCA'G

Cuts at:      0     530    1133    1391    2094 
   Size:        530     603     258     703

  Fragments arranged by size: 

                 703     603     530     258
 
PvuII CAG'CTG

Cuts at:      0    1643    2094 
   Size:       1643     451
 
SacII CC_GC'GG

Cuts at:      0      85     103    2094 
   Size:         85      18    1991

  Fragments arranged by size: 

                1991      85      18
 
SexAI A'CCwGG_T

Cuts at:      0    1187    1833    2094 
   Size:       1187     646     261

  Fragments arranged by size: 

                1187     646     261
 
SfcI C'TryA_G

Cuts at:      0     526    1129    1387    2094 
   Size:        526     603     258     707

  Fragments arranged by size: 

                 707     603     526     258
 
SmaI CCC'GGG

Cuts at:      0      35    1917    2094 
   Size:         35    1882     177

  Fragments arranged by size: 

                1882     177      35
 
SmlI C'TyrA_G

Cuts at:      0    1057    2094 
   Size:       1057    1037
 
SspI AAT'ATT

Cuts at:      0    1220    2094 
   Size:       1220     874
 
StuI AGG'CCT

Cuts at:      0    1854    2094 
   Size:       1854     240
 
StyI C'CwwG_G

Cuts at:      0     663    1469    1800    2094 
   Size:        663     806     331     294

  Fragments arranged by size: 

                 806     663     331     294
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     180     500    1352    1412    1823    1831    2094 
   Size:        180     320     852      60     411       8     263

  Fragments arranged by size: 

                 852     411     320     263     180      60       8
 
TatI w'GTAC_w

Cuts at:      0     965    2033    2094 
   Size:        965    1068      61

  Fragments arranged by size: 

                1068     965      61
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     172     204     659     691    2094 
   Size:        172      32     455      32    1403

  Fragments arranged by size: 

                1403     455     172      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1189    2094 
   Size:       1189     905
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1152    1583    1906    2094 
   Size:       1152     431     323     188

  Fragments arranged by size: 

                1152     431     323     188
 
XmaI C'CCGG_G

Cuts at:      0      33    1915    2094 
   Size:         33    1882     179

  Fragments arranged by size: 

                1882     179      33
 
XmnI GAAnn'nnTTC

Cuts at:      0    1303    2094 
   Size:       1303     791
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI    AlwNI     ApaI    ApaLI     ApoI     AvaI
    BamHI    BanII     BbsI     BglI Bme1580I     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstEII    BstYI     BtgI     BtsI
     DraI   DraIII     EaeI     EagI     EarI  Eco57MI EcoO109I    HaeII
    Hin4I   HincII  HindIII     MmeI     MscI     MslI   MspA1I     NaeI
     NcoI   NgoMIV     NheI    PflMI     PmlI    PpuMI    PshAI   PspOMI
     PstI    PvuII    SacII    SexAI     SfcI     SmaI     SmlI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AleI     AlfI     AloI     AscI     AseI    AsiSI    AvrII     BaeI
     BanI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI    BmgBI     BplI   Bpu10I    BsaBI    BsiWI     BsmI    BsmBI
    BspHI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DrdI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI     HpaI     KasI     KpnI     MfeI     MluI
     NarI     NdeI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PpiI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SwaI
     XbaI     XhoI     ZraI
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