Restriction map for pF1KB5657
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# NO CUT ENZYMES (  120 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        AspEI       AvaI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbvCI       
BcgI        BfrI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaHI       BsaWI       BseAI       BsiWI       
BsmBI       BsoBI       Bsp1407I    BspEI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstEII      BstPI       
BstZ17I     CelII       Cfr10I      DraII       DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO109I    EcoO65I     
EcoRV       FseI        FspI        HaeII       HgaI        
Hin1I       HpaI        KasI        KspI        MfeI        
MluI        MluNI       MroI        MscI        MspA1I      
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NotI        NruI        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshBI       Psp1406I    PvuII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
SwaI        XbaI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   38 enzymes) #
###################################

AocI          130  CCTCAGG              CC^T[ACGT]AGG
AspHI        2120  GAGCAC               G[AT]GC[AT]^C
AspI          220  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BglI          113  GCCGCGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI        2881  GATGATAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1989  GGTCTC               GGTCTC(1/5)
BseRI        3072  CTCCTC               GAGGAG(10/8)
BsgI          385  GTGCAG               GTGCAG(16/14)
BsiEI        1491  CGATCG               CG[GA][CT]^CG
BsiHKAI      2120  GAGCAC               G[AT]GC[AT]^C
BsmI         3351  GCATTC               GAATGC(1/-1)
BspDI        1449  ATCGAT               AT^CGAT
BstBI        2939  TTCGAA               TT^CGAA
Bsu36I        130  CCTCAGG              CC^T[ACGT]AGG
ClaI         1449  ATCGAT               AT^CGAT
CpoI          847  CGGTCCG              CG^G[AT]CCG
DraIII        359  CACAAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I        130  CCTCAGG              CC^T[ACGT]AGG
EcoT22I      3349  ATGCAT               ATGCA^T
FauI          108  CCCGC                CCCGC(4/6)
HincII        331  GTTGAC               GT[CT]^[GA]AC
HindII        331  GTTGAC               GT[CT]^[GA]AC
HindIII      1234  AAGCTT               A^AGCTT
MaeII        1119  ACGT                 A^CGT
MamI         2881  GATGATAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI          183  GCTAGC               G^CTAGC
NsiI         3349  ATGCAT               ATGCA^T
NspI          923  ACATGC               [GA]CATG^[CT]
NspV         2939  TTCGAA               TT^CGAA
PflFI         220  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         427  CCAGATGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI          79  GACCGGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          338  CTGCAG               CTGCA^G
PvuI         1491  CGATCG               CGAT^CG
RsrII         847  CGGTCCG              CG^G[AT]CCG
SfuI         2939  TTCGAA               TT^CGAA
Tth111I       220  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        427  CCAGATGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccII         110  CGCG                 CG^CG
              115  CGCG                
AlwNI         814  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1718  CAGGAACTG           
Asp700I       490  GAACCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              543  GAAGACTTTC          
BanII         120  GGGCTC               G[GA]GC[CT]^C
             1196  GGGCTC              
BciVI        2073  GGATAC               GTATCC(6/5)
             2906  GTATCC              
BcnI           81  CCGGG                CC^[GC]GG
             3361  CCCGG               
BglII         644  AGATCT               A^GATCT
             2108  AGATCT              
BspHI        1704  TCATGA               T^CATGA
             3035  TCATGA              
BsrDI        2635  GCAATG               GCAATG(2/0)
             3176  GCAATG              
BstUI         110  CGCG                 CG^CG
              115  CGCG                
BstXI         285  CCAAGGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2594  CCAATATTGTGG        
EarI         2425  CTCTTC               CTCTTC(1/4)
             2732  CTCTTC              
EcoRI        1347  GAATTC               G^AATTC
             3100  GAATTC              
EcoT14I       285  CCAAGG               C^C[AT][AT]GG
             1939  CCAAGG              
Ksp632I      2425  CTCTTC               CTCTTC(1/4)
             2732  CTCTTC              
MvnI          110  CGCG                 CG^CG
              115  CGCG                
MwoI          113  GCCGCGAGGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              275  GCAATTTCAGC         
NciI           81  CCGGG                CC^[GC]GG
             3361  CCCGG               
RcaI         1704  TCATGA               T^CATGA
             3035  TCATGA              
SfcI          338  CTGCAG               C^T[GA][CT]AG
             1053  CTACAG              
SspI          211  AATATT               AAT^ATT
             2596  AATATT              
StyI          285  CCAAGG               C^C[AT][AT]GG
             1939  CCAAGG              
XmnI          490  GAACCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              543  GAAGACTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

Acc65I      AciI        AcsI        AfaI        AluI        
AlwI        ApoI        Asp718I     AvaII       BanI        
BbsI        BbvI        BclI        BfaI        BmyI        
BpmI        BpuAI       BsaJI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        EcoRII      
FbaI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        KpnI        MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspI        MvaI        NdeII       NlaIII      NlaIV       
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SmlI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        XhoII       
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