Restriction map for pF1KB5657
 (Linear) (Six-base) MAPSORT of: KIBB5657.seq  Check: 8431  from: 1  to: 3432



KIBB5657.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

Acc65I G'GTAC_C

Cuts at:      0    1753    1943    2693    3432 
   Size:       1753     190     750     739

  Fragments arranged by size: 

                1753     750     739     190
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2018    2282    3432 
   Size:       2018     264    1150

  Fragments arranged by size: 

                2018    1150     264
 
AleI CACnn'nnGTG

Cuts at:      0    1076    3432 
   Size:       1076    2356
 
AlwNI CAG_nnn'CTG

Cuts at:      0     819    1723    3432 
   Size:        819     904    1709

  Fragments arranged by size: 

                1709     904     819
 
ApoI r'AATT_y

Cuts at:      0    1347    1586    1636    1683    1800    2178    2231    2642 
   Size:       1347     239      50      47     117     378      53     411

Cuts at:   2642    3100    3432 
   Size:        458     332

  Fragments arranged by size: 

                1347     458     411     378     332     239     117      53
                  50      47
 
BanI G'GyrC_C

Cuts at:      0     510    1753    1943    2416    2693    3432 
   Size:        510    1243     190     473     277     739

  Fragments arranged by size: 

                1243     739     510     473     277     190
 
BanII G_rGCy'C

Cuts at:      0     124    1200    3432 
   Size:        124    1076    2232

  Fragments arranged by size: 

                2232    1076     124
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     550     620    1114    2874    3432 
   Size:        550      70     494    1760     558

  Fragments arranged by size: 

                1760     558     550     494      70
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2067    2917    3432 
   Size:       2067     850     515

  Fragments arranged by size: 

                2067     850     515
 
BclI T'GATC_A

Cuts at:      0     792    1500    2307    3432 
   Size:        792     708     807    1125

  Fragments arranged by size: 

                1125     807     792     708
 
BfrBI ATG'CAT

Cuts at:      0    3351    3432 
   Size:       3351      81
 
BglI GCCn_nnn'nGGC

Cuts at:      0     119    3432 
   Size:        119    3313
 
BglII A'GATC_T

Cuts at:      0     644    2108    3432 
   Size:        644    1464    1324

  Fragments arranged by size: 

                1464    1324     644
 
Bme1580I G_kGCm'C

Cuts at:      0    3185    3432 
   Size:       3185     247
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1743    3432 
   Size:       1743    1689
 
BmtI G_CTAG'C

Cuts at:      0     187    3432 
   Size:        187    3245
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     222    1631    3432 
   Size:        222    1409    1801

  Fragments arranged by size: 

                1801    1409     222
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1148    1530    1888    3432 
   Size:       1148     382     358    1544

  Fragments arranged by size: 

                1544    1148     382     358
 
Bpu10I CC'TnA_GC

Cuts at:      0    3013    3432 
   Size:       3013     419
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2917    3106    3411    3432 
   Size:       2917     189     305      21

  Fragments arranged by size: 

                2917     305     189      21
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1995    3432 
   Size:       1995    1437
 
BsaBI GATnn'nnATC

Cuts at:      0    2885    3432 
   Size:       2885     547
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1927    1957    3432 
   Size:       1927      30    1475

  Fragments arranged by size: 

                1927    1475      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    3063    3432 
   Size:       3063     369
 
BseYI C'CCAG_C

Cuts at:      0     746     854    3432 
   Size:        746     108    2578

  Fragments arranged by size: 

                2578     746     108
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     406    3432 
   Size:        406    3026
 
BsiEI CG_ry'CG

Cuts at:      0    1494    3432 
   Size:       1494    1938
 
BsiHKAI G_wGCw'C

Cuts at:      0    2124    3432 
   Size:       2124    1308
 
BsmI GAATG_Cn'

Cuts at:      0    3351    3432 
   Size:       3351      81
 
Bsp1286I G_dGCh'C

Cuts at:      0     124    1200    2124    3185    3432 
   Size:        124    1076     924    1061     247

  Fragments arranged by size: 

                1076    1061     924     247     124
 
BspHI T'CATG_A

Cuts at:      0    1704    3035    3432 
   Size:       1704    1331     397

  Fragments arranged by size: 

                1704    1331     397
 
BsrDI GCAATG_nn'

Cuts at:      0    2642    3183    3432 
   Size:       2642     541     249

  Fragments arranged by size: 

                2642     541     249
 
BstBI TT'CG_AA

Cuts at:      0    2940    3432 
   Size:       2940     492
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     292    2601    3432 
   Size:        292    2309     831

  Fragments arranged by size: 

                2309     831     292
 
BstYI r'GATC_y

Cuts at:      0     644    1008    1015    2108    3432 
   Size:        644     364       7    1093    1324

  Fragments arranged by size: 

                1324    1093     644     364       7
 
Bsu36I CC'TnA_GG

Cuts at:      0     131    3432 
   Size:        131    3301
 
BtsI GCAGTG_nn'

Cuts at:      0      39     335     967    2797    3432 
   Size:         39     296     632    1830     635

  Fragments arranged by size: 

                1830     635     632     296      39
 
ClaI AT'CG_AT

Cuts at:      0    1450    3432 
   Size:       1450    1982
 
DraI TTT'AAA

Cuts at:      0    1116    1365    3302    3432 
   Size:       1116     249    1937     130

  Fragments arranged by size: 

                1937    1116     249     130
 
DraIII CAC_nnn'GTG

Cuts at:      0     364    3432 
   Size:        364    3068
 
EarI CTCTTCn'nnn_

Cuts at:      0    2431    2738    3432 
   Size:       2431     307     694

  Fragments arranged by size: 

                2431     694     307
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1148    1530    1888    2018    2282    3432 
   Size:       1148     382     358     130     264    1150

  Fragments arranged by size: 

                1150    1148     382     358     264     130
 
EcoRI G'AATT_C

Cuts at:      0    1347    3100    3432 
   Size:       1347    1753     332

  Fragments arranged by size: 

                1753    1347     332
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     209     241    1000    1032    1834    1866    1966    1998 
   Size:        209      32     759      32     802      32     100      32

Cuts at:   1998    3432 
   Size:       1434

  Fragments arranged by size: 

                1434     802     759     209     100      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     333    3432 
   Size:        333    3099
 
HindIII A'AGCT_T

Cuts at:      0    1234    3432 
   Size:       1234    2198
 
KpnI G_GTAC'C

Cuts at:      0    1757    1947    2697    3432 
   Size:       1757     190     750     735

  Fragments arranged by size: 

                1757     750     735     190
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     374    2571    3432 
   Size:        374    2197     861

  Fragments arranged by size: 

                2197     861     374
 
MslI CAynn'nnrTG

Cuts at:      0    1076    1706    3055    3188    3432 
   Size:       1076     630    1349     133     244

  Fragments arranged by size: 

                1349    1076     630     244     133
 
NheI G'CTAG_C

Cuts at:      0     183    3432 
   Size:        183    3249
 
NsiI A_TGCA'T

Cuts at:      0    3353    3432 
   Size:       3353      79
 
NspI r_CATG'y

Cuts at:      0     927    3432 
   Size:        927    2505
 
PasI CC'CwG_GG

Cuts at:      0    1148    3432 
   Size:       1148    2284
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     433    3432 
   Size:        433    2999
 
PfoI T'CCnGG_A

Cuts at:      0    1125    1176    1865    2774    2909    3432 
   Size:       1125      51     689     909     135     523

  Fragments arranged by size: 

                1125     909     689     523     135      51
 
PshAI GACnn'nnGTC

Cuts at:      0      83    3432 
   Size:         83    3349
 
PstI C_TGCA'G

Cuts at:      0     342    3432 
   Size:        342    3090
 
PvuI CG_AT'CG

Cuts at:      0    1494    3432 
   Size:       1494    1938
 
RsrII CG'GwC_CG

Cuts at:      0     848    3432 
   Size:        848    2584
 
SfcI C'TryA_G

Cuts at:      0     338    1053    3432 
   Size:        338     715    2379

  Fragments arranged by size: 

                2379     715     338
 
SmlI C'TyrA_G

Cuts at:      0    2896    3085    3390    3432 
   Size:       2896     189     305      42

  Fragments arranged by size: 

                2896     305     189      42
 
SspI AAT'ATT

Cuts at:      0     213    2598    3432 
   Size:        213    2385     834

  Fragments arranged by size: 

                2385     834     213
 
StyI C'CwwG_G

Cuts at:      0     285    1939    3432 
   Size:        285    1654    1493

  Fragments arranged by size: 

                1654    1493     285
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     836    1725    1991    2982    3432 
   Size:        836     889     266     991     450

  Fragments arranged by size: 

                 991     889     836     450     266
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2030    2062    2132    2164    2567    2599    3432 
   Size:       2030      32      70      32     403      32     833

  Fragments arranged by size: 

                2030     833     403      70      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     223    3432 
   Size:        223    3209
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1254    1683    2760    3432 
   Size:       1254     429    1077     672

  Fragments arranged by size: 

                1254    1077     672     429
 
XmnI GAAnn'nnTTC

Cuts at:      0     494     547    3432 
   Size:        494      53    2885

  Fragments arranged by size: 

                2885     494      53
 

 Enzymes that do cut:

   Acc65I     AcuI     AleI    AlwNI     ApoI     BanI    BanII     BbsI
    BciVI     BclI    BfrBI     BglI    BglII Bme1580I     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrDI
    BstBI    BstXI    BstYI   Bsu36I     BtsI     ClaI     DraI   DraIII
     EarI  Eco57MI    EcoRI    Hin4I   HincII  HindIII     KpnI     MmeI
     MslI     NheI     NsiI     NspI     PasI    PflMI     PfoI    PshAI
     PstI     PvuI    RsrII     SfcI     SmlI     SspI     StyI    TaqII
     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI     BlpI
    BmgBI    BsaAI    BsaHI    BsaWI    BsiWI    BsmBI    BspEI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII  BstZ17I
     BtgI    BtgZI    CspCI     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII
     HpaI     KasI     MfeI     MluI     MscI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NotI     NruI     PacI     PciI     PmeI
     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI    PvuII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI     TatI     XbaI     XhoI     XmaI     ZraI
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