Restriction map for pF1KB5565
##################################
# NO CUT ENZYMES (  114 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
AocI        Aor51HI     ApaI        AscI        Asp718I     
AspEI       AspI        AviII       AvrII       BanI        
BanII       BbeI        BbrPI       BfrI        BglI        
BglII       BlnI        BsaAI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BspEI       BspLU11I    BspMI       BsrBI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     Bsu36I      
Cfr10I      CpoI        DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     FauI        FseI        FspI        
HgaI        Hin1I       HindIII     HpaI        KasI        
KpnI        KspI        MluI        MroI        MvnI        
NaeI        NarI        NcoI        NgoMIV      NheI        
NotI        NruI        NspI        NspV        PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    StuI        SwaI        
Tth111I     XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   64 enzymes) #
###################################

AccI          859  GTCGAC               GT^[AC][GT]AC
Alw44I        469  GTGCAC               G^TGCAC
AlwNI          25  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         469  GTGCAC               G^TGCAC
AspHI         469  GTGCAC               G[AT]GC[AT]^C
AvaI           93  CTCGAG               C^[CT]CG[GA]G
BalI          433  TGGCCA               TGG^CCA
BamHI        1461  GGATCC               G^GATCC
BbsI         2047  GAAGAC               GAAGAC(2/6)
BbvCI          22  CCTCAGC              CCTCAGC(-5/-2)
BcgI          861  CGACTTGTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI         1878  CCGGG                CC^[GC]GG
BlpI         1048  GCTAAGC              GC^T[ACGT]AGC
BmyI          469  GTGCAC               G[AGT]GC[ACT]^C
Bpu1102I     1048  GCTAAGC              GC^T[ACGT]AGC
BpuAI        2047  GAAGAC               GAAGAC(2/6)
BseRI        1244  CTCCTC               GAGGAG(10/8)
BsgI          834  CTGCAC               GTGCAG(16/14)
BsiHKAI       469  GTGCAC               G[AT]GC[AT]^C
BsmAI        2136  GAGAC                GTCTC(1/5)
BsmI          334  GAATGC               GAATGC(1/-1)
BsoBI          93  CTCGAG               C^[CT]CG[GA]G
Bsp1286I      469  GTGCAC               G[AGT]GC[ACT]^C
Bsp1407I     1511  TGTACA               T^GTACA
BspDI         158  ATCGAT               AT^CGAT
BsrGI        1511  TGTACA               T^GTACA
BstXI        1112  CCAGAAACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I        1915  GCAGGC               GC[ACGT]^[ACGT]GC
CelII        1048  GCTAAGC              GC^T[ACGT]AGC
CfoI           40  GCGC                 GCG^C
ClaI          158  ATCGAT               AT^CGAT
DraII         200  AGGTCCT              [GA]G^G[ACGT]CC[CT]
DraIII        400  CACAAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          433  TGGCCA               [CT]^GGCC[GA]
EarI         1985  CTCTTC               CTCTTC(1/4)
EcoO109I      200  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRV        1828  GATATC               GAT^ATC
EcoT22I      2125  ATGCAT               ATGCA^T
HaeII          39  GGCGCT               [GA]GCGC^[CT]
HapII        1878  CCGG                 C^CGG
HhaI           40  GCGC                 GCG^C
HinP1I         40  GCGC                 G^CGC
HpaII        1878  CCGG                 C^CGG
Ksp632I      1985  CTCTTC               CTCTTC(1/4)
MfeI         2085  CAATTG               C^AATTG
MluNI         433  TGGCCA               TGG^CCA
MscI          433  TGGCCA               TGG^CCA
MspA1I         25  CAGCTG               C[AC]G^C[GT]G
MspI         1878  CCGG                 C^CGG
MunI         2085  CAATTG               C^AATTG
NciI         1878  CCGGG                CC^[GC]GG
NdeI          521  CATATG               CA^TATG
NsiI         2125  ATGCAT               ATGCA^T
PacI         1396  TTAATTAA             TTAAT^TAA
PaeR7I         93  CTCGAG               C^TCGAG
PpuMI         200  AGGTCCT              [GA]G^G[AT]CC[CT]
PstI          869  CTGCAG               CTGCA^G
PvuII          25  CAGCTG               CAG^CTG
SalI          859  GTCGAC               G^TCGAC
SmlI           93  CTCGAG               C^T[CT][GA]AG
SspBI        1511  TGTACA               T^GTACA
SspI          179  AATATT               AAT^ATT
Tsp45I       2100  GTCAC                ^GT[GC]AC
XhoI           93  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AciI           76  GCGG                 CCGC(-3/-1)
             1105  GCGG                
BciVI        1306  GGATAC               GTATCC(6/5)
              459  GTATCC              
BclI          303  TGATCA               T^GATCA
              570  TGATCA              
BfaI          448  CTAG                 C^TAG
             1490  CTAG                
BsaBI         565  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              877  GATGTCCATC          
BsmFI        1372  GTCCC                GGGAC(10/14)
             1816  GTCCC               
BstYI        1461  GGATCC               [GA]^GATC[CT]
             2205  AGATCC              
DraI          690  TTTAAA               TTT^AAA
             1206  TTTAAA              
EcoRI         492  GAATTC               G^AATTC
              847  GAATTC              
EcoT14I       204  CCTTGG               C^C[AT][AT]GG
             1198  CCAAGG              
FbaI          303  TGATCA               T^GATCA
              570  TGATCA              
HaeIII        259  GGCC                 GG^CC
              434  GGCC                
MaeI          448  CTAG                 C^TAG
             1490  CTAG                
MaeII         890  ACGT                 A^CGT
             2039  ACGT                
MamI          565  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              877  GATGTCCATC          
MflI         1461  GGATCC               [GA]^GATC[CT]
             2205  AGATCC              
MwoI           21  GCCTCAGCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               33  GCTCCTGGCGC         
StyI          204  CCTTGG               C^C[AT][AT]GG
             1198  CCAAGG              
XhoII        1461  GGATCC               [GA]^GATC[CT]
             2205  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
AseI        AsnI        Asp700I     AvaII       BbvI        
BpmI        BsaJI       BsiYI       BslI        BspHI       
BsrDI       BsrI        BssKI       BstF5I      BstNI       
Cfr13I      DdeI        DpnII       EcoRII      Fnu4HI      
FokI        HincII      HindII      HinfI       HphI        
ItaI        MaeIII      MboI        MboII       MnlI        
MseI        MslI        MvaI        NdeII       NlaIII      
NlaIV       PflMI       PleI        PshBI       RcaI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TaqI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XmnI        
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