Restriction map for pF1KB5565
 (Linear) (Six-base) MAPSORT of: KIBB5565.seq  Check: 2799  from: 1  to: 2211



KIBB5565.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AccI GT'mk_AC

Cuts at:      0     860    2211 
   Size:        860    1351
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1343    1924    2211 
   Size:       1343     581     287

  Fragments arranged by size: 

                1343     581     287
 
AlwNI CAG_nnn'CTG

Cuts at:      0      30    2211 
   Size:         30    2181
 
ApaLI G'TGCA_C

Cuts at:      0     469    2211 
   Size:        469    1742
 
ApoI r'AATT_y

Cuts at:      0     382     425     492     650     694     847    1211    1531 
   Size:        382      43      67     158      44     153     364     320

Cuts at:   1531    1947    2071    2211 
   Size:        416     124     140

  Fragments arranged by size: 

                 416     382     364     320     158     153     140     124
                  67      44      43
 
AseI AT'TA_AT

Cuts at:      0     242     274     755    2211 
   Size:        242      32     481    1456

  Fragments arranged by size: 

                1456     481     242      32
 
AvaI C'yCGr_G

Cuts at:      0      93    2211 
   Size:         93    2118
 
BamHI G'GATC_C

Cuts at:      0    1461    2211 
   Size:       1461     750
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2054    2211 
   Size:       2054     157
 
BbvCI CC'TCA_GC

Cuts at:      0      23    2211 
   Size:         23    2188
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     850     884    2211 
   Size:        850      34    1327

  Fragments arranged by size: 

                1327     850      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     470    1300    2211 
   Size:        470     830     911

  Fragments arranged by size: 

                 911     830     470
 
BclI T'GATC_A

Cuts at:      0     303     570    2211 
   Size:        303     267    1641

  Fragments arranged by size: 

                1641     303     267
 
BfrBI ATG'CAT

Cuts at:      0    2127    2211 
   Size:       2127      84
 
BlpI GC'TnA_GC

Cuts at:      0    1049    2211 
   Size:       1049    1162
 
Bme1580I G_kGCm'C

Cuts at:      0     473    2211 
   Size:        473    1738
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     108    2211 
   Size:        108    2103
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     538    1873    2136    2211 
   Size:        538    1335     263      75

  Fragments arranged by size: 

                1335     538     263      75
 
Bpu10I CC'TnA_GC

Cuts at:      0      23    2211 
   Size:         23    2188
 
BsaBI GATnn'nnATC

Cuts at:      0     569     881    2211 
   Size:        569     312    1330

  Fragments arranged by size: 

                1330     569     312
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     216     246    2211 
   Size:        216      30    1965

  Fragments arranged by size: 

                1965     216      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1235    2211 
   Size:       1235     976
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     819    2211 
   Size:        819    1392
 
BsiHKAI G_wGCw'C

Cuts at:      0     473    2211 
   Size:        473    1738
 
BsmI GAATG_Cn'

Cuts at:      0     340    2211 
   Size:        340    1871
 
Bsp1286I G_dGCh'C

Cuts at:      0     473    2211 
   Size:        473    1738
 
BspHI T'CATG_A

Cuts at:      0    1014    1782    1998    2211 
   Size:       1014     768     216     213

  Fragments arranged by size: 

                1014     768     216     213
 
BsrDI GCAATG_nn'

Cuts at:      0    1238    1886    2024    2186    2211 
   Size:       1238     648     138     162      25

  Fragments arranged by size: 

                1238     648     162     138      25
 
BsrGI T'GTAC_A

Cuts at:      0    1511    2211 
   Size:       1511     700
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1119    2211 
   Size:       1119    1092
 
BstYI r'GATC_y

Cuts at:      0    1461    2205    2211 
   Size:       1461     744       6

  Fragments arranged by size: 

                1461     744       6
 
BtsI GCAGTG_nn'

Cuts at:      0    1550    2198    2211 
   Size:       1550     648      13

  Fragments arranged by size: 

                1550     648      13
 
ClaI AT'CG_AT

Cuts at:      0     159    2211 
   Size:        159    2052
 
DraI TTT'AAA

Cuts at:      0     692    1208    2211 
   Size:        692     516    1003

  Fragments arranged by size: 

                1003     692     516
 
DraIII CAC_nnn'GTG

Cuts at:      0     405    2211 
   Size:        405    1806
 
EaeI y'GGCC_r

Cuts at:      0     433    2211 
   Size:        433    1778
 
EarI CTCTTCn'nnn_

Cuts at:      0    1991    2211 
   Size:       1991     220
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     538    1343    1873    1924    2136    2211 
   Size:        538     805     530      51     212      75

  Fragments arranged by size: 

                 805     538     530     212      75      51
 
EcoO109I rG'GnC_Cy

Cuts at:      0     201    2211 
   Size:        201    2010
 
EcoRI G'AATT_C

Cuts at:      0     492     847    2211 
   Size:        492     355    1364

  Fragments arranged by size: 

                1364     492     355
 
EcoRV GAT'ATC

Cuts at:      0    1830    2211 
   Size:       1830     381
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     997    2211 
   Size:        997    1214
 
HaeII r_GCGC'y

Cuts at:      0      43    2211 
   Size:         43    2168
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1227    1259    2211 
   Size:       1227      32     952

  Fragments arranged by size: 

                1227     952      32
 
HincII GTy'rAC

Cuts at:      0     528     861     956    2211 
   Size:        528     333      95    1255

  Fragments arranged by size: 

                1255     528     333      95
 
MfeI C'AATT_G

Cuts at:      0    2085    2211 
   Size:       2085     126
 
MscI TGG'CCA

Cuts at:      0     435    2211 
   Size:        435    1776
 
MslI CAynn'nnrTG

Cuts at:      0    1123    2000    2150    2191    2211 
   Size:       1123     877     150      41      20

  Fragments arranged by size: 

                1123     877     150      41      20
 
MspA1I CmG'CkG

Cuts at:      0      27    2211 
   Size:         27    2184
 
NdeI CA'TA_TG

Cuts at:      0     522    2211 
   Size:        522    1689
 
NsiI A_TGCA'T

Cuts at:      0    2129    2211 
   Size:       2129      82
 
PacI TTA_AT'TAA

Cuts at:      0    1400    2211 
   Size:       1400     811
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     405     815    1283    2211 
   Size:        405     410     468     928

  Fragments arranged by size: 

                 928     468     410     405
 
PfoI T'CCnGG_A

Cuts at:      0     554    1877    2211 
   Size:        554    1323     334

  Fragments arranged by size: 

                1323     554     334
 
PpuMI rG'GwC_Cy

Cuts at:      0     201    2211 
   Size:        201    2010
 
PsiI TTA'TAA

Cuts at:      0    1577    2211 
   Size:       1577     634
 
PspXI vC'TCGA_Gb

Cuts at:      0      93    2211 
   Size:         93    2118
 
PstI C_TGCA'G

Cuts at:      0     873    2211 
   Size:        873    1338
 
PvuII CAG'CTG

Cuts at:      0      27    2211 
   Size:         27    2184
 
SalI G'TCGA_C

Cuts at:      0     859    2211 
   Size:        859    1352
 
SfcI C'TryA_G

Cuts at:      0     128     674     869    2211 
   Size:        128     546     195    1342

  Fragments arranged by size: 

                1342     546     195     128
 
SmlI C'TyrA_G

Cuts at:      0      93    2211 
   Size:         93    2118
 
SspI AAT'ATT

Cuts at:      0     181    2211 
   Size:        181    2030
 
StyI C'CwwG_G

Cuts at:      0     204    1198    2211 
   Size:        204     994    1013

  Fragments arranged by size: 

                1013     994     204
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      61    1489    2211 
   Size:         61    1428     722

  Fragments arranged by size: 

                1428     722      61
 
TatI w'GTAC_w

Cuts at:      0    1391    1511    2211 
   Size:       1391     120     700

  Fragments arranged by size: 

                1391     700     120
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      31     216     248    1135    1167    2211 
   Size:         31     185      32     887      32    1044

  Fragments arranged by size: 

                1044     887     185      32      32      31
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     664    1694    2111    2211 
   Size:        664    1030     417     100

  Fragments arranged by size: 

                1030     664     417     100
 
XhoI C'TCGA_G

Cuts at:      0      93    2211 
   Size:         93    2118
 
XmnI GAAnn'nnTTC

Cuts at:      0     371    2051    2159    2211 
   Size:        371    1680     108      52

  Fragments arranged by size: 

                1680     371     108      52
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI    ApaLI     ApoI     AseI     AvaI    BamHI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BlpI Bme1580I
     BplI     BpmI   Bpu10I    BsaBI    BsaXI    BseRI     BsgI  BsiHKAI
     BsmI Bsp1286I    BspHI    BsrDI    BsrGI    BstXI    BstYI     BtsI
     ClaI     DraI   DraIII     EaeI     EarI  Eco57MI EcoO109I    EcoRI
    EcoRV     FalI    HaeII    Hin4I   HincII     MfeI     MscI     MslI
   MspA1I     NdeI     NsiI     PacI    PflMI     PfoI    PpuMI     PsiI
    PspXI     PstI    PvuII     SalI     SfcI     SmlI     SspI     StyI
    TaqII     TatI     TstI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI     AscI    AsiSI    AvrII
     BaeI     BanI    BanII     BbeI     BglI    BglII    BmgBI     BmrI
     BmtI    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BseYI    BsiEI
    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI    CspCI
     DrdI     EagI     EciI  EcoICRI    EcoNI     FseI     FspI    FspAI
  HindIII     HpaI     KasI     KpnI     MluI     MmeI     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     NspI     PasI     PciI
     PmeI     PmlI     PpiI    PshAI   PspOMI     PsrI     PvuI    RsrII
     SacI    SacII    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI  Tth111I     XbaI     XmaI     ZraI
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