Restriction map for pF1KB4146
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        AocI        
Aor51HI     ApaI        AscI        AseI        AsnI        
AspEI       AspI        AviII       AvrII       BbeI        
BbrPI       BbvCI       BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
Bsp1407I    BspDI       BspEI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       FauI        FseI        FspI        
HaeII       Hin1I       HpaI        KasI        KspI        
MamI        MfeI        MluI        MroI        MunI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       StuI        SwaI        Tth111I     
XbaI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   45 enzymes) #
###################################

Acc65I        604  GGTACC               G^GTACC
AlwNI        1232  CAGTGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp718I       604  GGTACC               G^GTACC
AvaI           55  CCCGAG               C^[CT]CG[GA]G
AvaII         675  GGACC                G^G[AT]CC
BalI         1521  TGGCCA               TGG^CCA
BamHI         800  GGATCC               G^GATCC
BanI          604  GGTACC               G^G[CT][GA]CC
BciVI        1565  GGATAC               GTATCC(6/5)
BclI          522  TGATCA               T^GATCA
BfaI          679  CTAG                 C^TAG
BglII         579  AGATCT               A^GATCT
BseRI        1345  CTCCTC               GAGGAG(10/8)
BsgI          310  CTGCAC               GTGCAG(16/14)
BsmBI         913  CGTCTC               CGTCTC(1/5)
BsoBI          55  CCCGAG               C^[CT]CG[GA]G
BspHI         980  TCATGA               T^CATGA
CfoI         1047  GCGC                 GCG^C
DraII         674  GGGACCT              [GA]G^G[ACGT]CC[CT]
DrdI          676  GACCTAGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         1521  TGGCCA               [CT]^GGCC[GA]
EarI          562  GAAGAG               CTCTTC(1/4)
EcoO109I      674  GGGACCT              [GA]G^G[ACGT]CC[CT]
FbaI          522  TGATCA               T^GATCA
HgaI         1227  GCGTC                GACGC(5/10)
HhaI         1047  GCGC                 GCG^C
HinP1I       1047  GCGC                 G^CGC
HindIII       644  AAGCTT               A^AGCTT
KpnI          604  GGTACC               GGTAC^C
Ksp632I       562  GAAGAG               CTCTTC(1/4)
MaeI          679  CTAG                 C^TAG
MluNI        1521  TGGCCA               TGG^CCA
MscI         1521  TGGCCA               TGG^CCA
MslI         1703  CATACAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI         1308  GCATGC               [GA]CATG^[CT]
PflMI        1098  CCAAGAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         674  GGGACCT              [GA]G^G[AT]CC[CT]
PvuII         903  CAGCTG               CAG^CTG
RcaI          980  TCATGA               T^CATGA
SmlI          916  CTCAAG               C^T[CT][GA]AG
SphI         1308  GCATGC               GCATG^C
SspI          663  AATATT               AAT^ATT
TaqI         1614  TCGA                 T^CGA
TthHB8I      1614  TCGA                 T^CGA
Van91I       1098  CCAAGAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AccII        1046  CGCG                 CG^CG
             1048  CGCG                
AcsI          286  GAATTT               [GA]^AATT[CT]
             1728  AAATTC              
AfaI            3  GTAC                 GT^AC
              605  GTAC                
Alw44I        347  GTGCAC               G^TGCAC
              363  GTGCAC              
ApaLI         347  GTGCAC               G^TGCAC
              363  GTGCAC              
ApoI          286  GAATTT               [GA]^AATT[CT]
             1728  AAATTC              
Asp700I       418  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1543  GAATCTGTTC          
BanII         270  GGGCTC               G[GA]GC[CT]^C
             1142  GAGCCC              
BbsI           67  GAAGAC               GAAGAC(2/6)
              757  GTCTTC              
BcgI          315  CGACCACCTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1480  CGATCCATCTGC        
BcnI          672  CCGGG                CC^[GC]GG
             1388  CCCGG               
BpuAI          67  GAAGAC               GAAGAC(2/6)
              757  GTCTTC              
BsmFI         674  GGGAC                GGGAC(10/14)
              892  GGGAC               
BsmI          341  GAATGC               GAATGC(1/-1)
             1466  GCATTC              
BsrDI        1175  CATTGC               GCAATG(2/0)
              698  GCAATG              
BstUI        1046  CGCG                 CG^CG
             1048  CGCG                
BstXI         201  CCAAATGTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              234  CCAAGAATCTGG        
DraIII        687  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
              971  CACCAAGTG           
DsaI          471  CCATGG               C^C[GA][CT]GG
              795  CCATGG              
EcoT22I      1310  ATGCAT               ATGCA^T
             1464  ATGCAT              
HapII         672  CCGG                 C^CGG
             1389  CCGG                
HincII       1339  GTCAAC               GT[CT]^[GA]AC
             1639  GTCAAC              
HindII       1339  GTCAAC               GT[CT]^[GA]AC
             1639  GTCAAC              
HpaII         672  CCGG                 C^CGG
             1389  CCGG                
MaeII         912  ACGT                 A^CGT
             1135  ACGT                
MseI          538  TTAA                 T^TAA
             1138  TTAA                
MspA1I        903  CAGCTG               C[AC]G^C[GT]G
             1603  CAGCGG              
MspI          672  CCGG                 C^CGG
             1389  CCGG                
MvnI         1046  CGCG                 CG^CG
             1048  CGCG                
NciI          672  CCGGG                CC^[GC]GG
             1388  CCCGG               
NcoI          471  CCATGG               C^CATGG
              795  CCATGG              
NsiI         1310  ATGCAT               ATGCA^T
             1464  ATGCAT              
RsaI            3  GTAC                 GT^AC
              605  GTAC                
Tru9I         538  TTAA                 T^TAA
             1138  TTAA                
XmnI          418  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1543  GAATCTGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AciI        AluI        AlwI        AspHI       BbvI        
BmyI        BpmI        BsaJI       BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
MaeIII      MboI        MboII       MflI        MnlI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PleI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TfiI        TseI        Tsp45I      
Tsp509I     TspRI       XcmI        XhoII       
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