Restriction map for pF1KB4146
 (Linear) (Six-base) MAPSORT of: KIBB4146.seq  Check: 5012  from: 1  to: 1857



KIBB4146.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

Acc65I G'GTAC_C

Cuts at:      0     604    1857 
   Size:        604    1253
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     409     962    1857 
   Size:        409     553     895

  Fragments arranged by size: 

                 895     553     409
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1094    1126    1857 
   Size:       1094      32     731

  Fragments arranged by size: 

                1094     731      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1237    1857 
   Size:       1237     620
 
ApaLI G'TGCA_C

Cuts at:      0     347     363    1857 
   Size:        347      16    1494

  Fragments arranged by size: 

                1494     347      16
 
ApoI r'AATT_y

Cuts at:      0     286    1728    1857 
   Size:        286    1442     129

  Fragments arranged by size: 

                1442     286     129
 
AvaI C'yCGr_G

Cuts at:      0      55    1857 
   Size:         55    1802
 
BamHI G'GATC_C

Cuts at:      0     800    1857 
   Size:        800    1057
 
BanI G'GyrC_C

Cuts at:      0     604    1857 
   Size:        604    1253
 
BanII G_rGCy'C

Cuts at:      0     274    1146    1857 
   Size:        274     872     711

  Fragments arranged by size: 

                 872     711     274
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      74     750    1857 
   Size:         74     676    1107

  Fragments arranged by size: 

                1107     676      74
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     304     338    1469    1503    1857 
   Size:        304      34    1131      34     354

  Fragments arranged by size: 

                1131     354     304      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1559    1857 
   Size:       1559     298
 
BclI T'GATC_A

Cuts at:      0     522    1857 
   Size:        522    1335
 
BfrBI ATG'CAT

Cuts at:      0    1312    1466    1857 
   Size:       1312     154     391

  Fragments arranged by size: 

                1312     391     154
 
BglII A'GATC_T

Cuts at:      0     579    1857 
   Size:        579    1278
 
Bme1580I G_kGCm'C

Cuts at:      0     351     367    1857 
   Size:        351      16    1490

  Fragments arranged by size: 

                1490     351      16
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     899    1759    1857 
   Size:        899     860      98

  Fragments arranged by size: 

                 899     860      98
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     214    1857 
   Size:        214    1643
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     209     712    1337    1595    1706    1857 
   Size:         46     163     503     625     258     111     151

  Fragments arranged by size: 

                 625     503     258     163     151     111      46
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     901    1857 
   Size:        901     956
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1568    1598    1857 
   Size:       1568      30     259

  Fragments arranged by size: 

                1568     259      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1336    1857 
   Size:       1336     521
 
BseYI C'CCAG_C

Cuts at:      0     722    1857 
   Size:        722    1135
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295    1857 
   Size:        295    1562
 
BsiHKAI G_wGCw'C

Cuts at:      0     351     367    1757    1857 
   Size:        351      16    1390     100

  Fragments arranged by size: 

                1390     351     100      16
 
BsmI GAATG_Cn'

Cuts at:      0     347    1466    1857 
   Size:        347    1119     391

  Fragments arranged by size: 

                1119     391     347
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     919    1857 
   Size:        919     938
 
Bsp1286I G_dGCh'C

Cuts at:      0     274     351     367    1146    1757    1857 
   Size:        274      77      16     779     611     100

  Fragments arranged by size: 

                 779     611     274     100      77      16
 
BspHI T'CATG_A

Cuts at:      0     980    1857 
   Size:        980     877
 
BsrDI GCAATG_nn'

Cuts at:      0     705    1174    1857 
   Size:        705     469     683

  Fragments arranged by size: 

                 705     683     469
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     208     241    1857 
   Size:        208      33    1616

  Fragments arranged by size: 

                1616     208      33
 
BstYI r'GATC_y

Cuts at:      0     579     800    1734    1857 
   Size:        579     221     934     123

  Fragments arranged by size: 

                 934     579     221     123
 
BtgI C'CryG_G

Cuts at:      0     471     795    1857 
   Size:        471     324    1062

  Fragments arranged by size: 

                1062     471     324
 
DraIII CAC_nnn'GTG

Cuts at:      0     692     976    1857 
   Size:        692     284     881

  Fragments arranged by size: 

                 881     692     284
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     682    1857 
   Size:        682    1175
 
EaeI y'GGCC_r

Cuts at:      0    1521    1857 
   Size:       1521     336
 
EarI CTCTTCn'nnn_

Cuts at:      0     557    1857 
   Size:        557    1300
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     209     409     712     962    1337    1595    1706 
   Size:         46     163     200     303     250     375     258     111

Cuts at:   1706    1857 
   Size:        151

  Fragments arranged by size: 

                 375     303     258     250     200     163     151     111
                  46
 
EcoO109I rG'GnC_Cy

Cuts at:      0     675    1857 
   Size:        675    1182
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     792     824    1528    1560    1857 
   Size:        792      32     704      32     297

  Fragments arranged by size: 

                 792     704     297      32      32
 
HincII GTy'rAC

Cuts at:      0    1341    1641    1857 
   Size:       1341     300     216

  Fragments arranged by size: 

                1341     300     216
 
HindIII A'AGCT_T

Cuts at:      0     644    1857 
   Size:        644    1213
 
KpnI G_GTAC'C

Cuts at:      0     608    1857 
   Size:        608    1249
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     833    1857 
   Size:        833    1024
 
MscI TGG'CCA

Cuts at:      0    1523    1857 
   Size:       1523     334
 
MslI CAynn'nnrTG

Cuts at:      0    1707    1857 
   Size:       1707     150
 
MspA1I CmG'CkG

Cuts at:      0     905    1605    1857 
   Size:        905     700     252

  Fragments arranged by size: 

                 905     700     252
 
NcoI C'CATG_G

Cuts at:      0     471     795    1857 
   Size:        471     324    1062

  Fragments arranged by size: 

                1062     471     324
 
NsiI A_TGCA'T

Cuts at:      0    1314    1468    1857 
   Size:       1314     154     389

  Fragments arranged by size: 

                1314     389     154
 
NspI r_CATG'y

Cuts at:      0    1312    1857 
   Size:       1312     545
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1104    1857 
   Size:       1104     753
 
PfoI T'CCnGG_A

Cuts at:      0    1387    1857 
   Size:       1387     470
 
PpuMI rG'GwC_Cy

Cuts at:      0     675    1857 
   Size:        675    1182
 
PsiI TTA'TAA

Cuts at:      0    1409    1857 
   Size:       1409     448
 
PvuII CAG'CTG

Cuts at:      0     905    1857 
   Size:        905     952
 
SfcI C'TryA_G

Cuts at:      0     292    1126    1299    1857 
   Size:        292     834     173     558

  Fragments arranged by size: 

                 834     558     292     173
 
SmlI C'TyrA_G

Cuts at:      0     916    1857 
   Size:        916     941
 
SphI G_CATG'C

Cuts at:      0    1312    1857 
   Size:       1312     545
 
SspI AAT'ATT

Cuts at:      0     665    1857 
   Size:        665    1192
 
StyI C'CwwG_G

Cuts at:      0     471     795    1499    1857 
   Size:        471     324     704     358

  Fragments arranged by size: 

                 704     471     358     324
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1111    1857 
   Size:       1111     746
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     452     484    1857 
   Size:        452      32    1373

  Fragments arranged by size: 

                1373     452      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     730    1491    1799    1857 
   Size:        730     761     308      58

  Fragments arranged by size: 

                 761     730     308      58
 
XmnI GAAnn'nnTTC

Cuts at:      0     422    1547    1857 
   Size:        422    1125     310

  Fragments arranged by size: 

                1125     422     310
 

 Enzymes that do cut:

   Acc65I     AcuI     AloI    AlwNI    ApaLI     ApoI     AvaI    BamHI
     BanI    BanII     BbsI     BcgI    BciVI     BclI    BfrBI    BglII
 Bme1580I     BmrI     BplI     BpmI    BpuEI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BsrDI    BstXI
    BstYI     BtgI   DraIII     DrdI     EaeI     EarI  Eco57MI EcoO109I
    Hin4I   HincII  HindIII     KpnI     MmeI     MscI     MslI   MspA1I
     NcoI     NsiI     NspI    PflMI     PfoI    PpuMI     PsiI    PvuII
     SfcI     SmlI     SphI     SspI     StyI    TaqII     TstI     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     ApaI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI    BbvCI     BglI     BlpI    BmgBI     BmtI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI    BspEI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII     HpaI     KasI     MfeI     MluI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     StuI     SwaI     TatI  Tth111I     XbaI
     XhoI     XmaI     ZraI
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