Restriction map for pF1KB3314
 (Linear) (Six-base) MAPSORT of: KIBB3314.seq  Check: 9366  from: 1  to: 2127



KIBB3314.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AatII G_ACGT'C

Cuts at:      0     518    1705    2127 
   Size:        518    1187     422

  Fragments arranged by size: 

                1187     518     422
 
Acc65I G'GTAC_C

Cuts at:      0    1572    2127 
   Size:       1572     555
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     152    1179    1688    2006    2127 
   Size:        152    1027     509     318     121

  Fragments arranged by size: 

                1027     509     318     152     121
 
AflII C'TTAA_G

Cuts at:      0     181    2127 
   Size:        181    1946
 
AflIII A'CryG_T

Cuts at:      0     580    1939    2127 
   Size:        580    1359     188

  Fragments arranged by size: 

                1359     580     188
 
AhdI GACnn_n'nnGTC

Cuts at:      0     279     504    1254    2127 
   Size:        279     225     750     873

  Fragments arranged by size: 

                 873     750     279     225
 
AlwNI CAG_nnn'CTG

Cuts at:      0     289    2127 
   Size:        289    1838
 
ApaLI G'TGCA_C

Cuts at:      0    1540    2127 
   Size:       1540     587
 
ApoI r'AATT_y

Cuts at:      0      28     189     556    1770    2127 
   Size:         28     161     367    1214     357

  Fragments arranged by size: 

                1214     367     357     161      28
 
AseI AT'TA_AT

Cuts at:      0    1357    2127 
   Size:       1357     770
 
BanI G'GyrC_C

Cuts at:      0    1572    2127 
   Size:       1572     555
 
BanII G_rGCy'C

Cuts at:      0     269    2127 
   Size:        269    1858
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1063    2127 
   Size:       1063    1064
 
BbvCI CC'TCA_GC

Cuts at:      0     100    2127 
   Size:        100    2027
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1531    1565    2127 
   Size:       1531      34     562

  Fragments arranged by size: 

                1531     562      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     246     649    2127 
   Size:        246     403    1478

  Fragments arranged by size: 

                1478     403     246
 
BclI T'GATC_A

Cuts at:      0     593    2127 
   Size:        593    1534
 
BglII A'GATC_T

Cuts at:      0     177     770     880    2127 
   Size:        177     593     110    1247

  Fragments arranged by size: 

                1247     593     177     110
 
Bme1580I G_kGCm'C

Cuts at:      0    1476    1544    2127 
   Size:       1476      68     583

  Fragments arranged by size: 

                1476     583      68
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      57      59    2127 
   Size:         57       2    2068

  Fragments arranged by size: 

                2068      57       2
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     787     910     996    1204    1727    2127 
   Size:        787     123      86     208     523     400

  Fragments arranged by size: 

                 787     523     400     208     123      86
 
Bpu10I CC'TnA_GC

Cuts at:      0     100     352    2127 
   Size:        100     252    1775

  Fragments arranged by size: 

                1775     252     100
 
BsaAI yAC'GTr

Cuts at:      0    1940    2127 
   Size:       1940     187
 
BsaBI GATnn'nnATC

Cuts at:      0    2045    2127 
   Size:       2045      82
 
BsaHI Gr'CG_yC

Cuts at:      0     515    1702    2127 
   Size:        515    1187     425

  Fragments arranged by size: 

                1187     515     425
 
BsaWI w'CCGG_w

Cuts at:      0      60     817    2127 
   Size:         60     757    1310

  Fragments arranged by size: 

                1310     757      60
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     412     442    2127 
   Size:        412      30    1685

  Fragments arranged by size: 

                1685     412      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     372     718    1201    1229    1597    1645    2127 
   Size:        372     346     483      28     368      48     482

  Fragments arranged by size: 

                 483     482     372     368     346      48      28
 
BseYI C'CCAG_C

Cuts at:      0     406    1221    2127 
   Size:        406     815     906

  Fragments arranged by size: 

                 906     815     406
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     421     605     929    2127 
   Size:        421     184     324    1198

  Fragments arranged by size: 

                1198     421     324     184
 
BsiHKAI G_wGCw'C

Cuts at:      0     269    1544    2127 
   Size:        269    1275     583

  Fragments arranged by size: 

                1275     583     269
 
Bsp1286I G_dGCh'C

Cuts at:      0     269    1476    1544    2127 
   Size:        269    1207      68     583

  Fragments arranged by size: 

                1207     583     269      68
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      62    2127 
   Size:         62    2065
 
BsrDI GCAATG_nn'

Cuts at:      0    1058    2127 
   Size:       1058    1069
 
BssSI C'ACGA_G

Cuts at:      0    1760    2127 
   Size:       1760     367
 
BstBI TT'CG_AA

Cuts at:      0     987    2127 
   Size:        987    1140
 
BstYI r'GATC_y

Cuts at:      0     177     608     770     880    2127 
   Size:        177     431     162     110    1247

  Fragments arranged by size: 

                1247     431     177     162     110
 
BtgI C'CryG_G

Cuts at:      0      93    2127 
   Size:         93    2034
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1147    2127 
   Size:       1147     980
 
BtsI GCAGTG_nn'

Cuts at:      0     404    2127 
   Size:        404    1723
 
ClaI AT'CG_AT

Cuts at:      0    1361    2127 
   Size:       1361     766
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     195     230    1248    1283    1743    1778    2127 
   Size:        195      35    1018      35     460      35     349

  Fragments arranged by size: 

                1018     460     349     195      35      35      35
 
DraI TTT'AAA

Cuts at:      0    1518    1986    2127 
   Size:       1518     468     141

  Fragments arranged by size: 

                1518     468     141
 
DraIII CAC_nnn'GTG

Cuts at:      0     399    2127 
   Size:        399    1728
 
EaeI y'GGCC_r

Cuts at:      0    1013    1622    2127 
   Size:       1013     609     505

  Fragments arranged by size: 

                1013     609     505
 
EcoICRI GAG'CTC

Cuts at:      0     267    2127 
   Size:        267    1860
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     152     787     910     996    1179    1204    1688    1727 
   Size:        152     635     123      86     183      25     484      39

Cuts at:   1727    2006    2127 
   Size:        279     121

  Fragments arranged by size: 

                 635     484     279     183     152     123     121      86
                  39      25
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     529     964    2127 
   Size:        529     435    1163

  Fragments arranged by size: 

                1163     529     435
 
EcoO109I rG'GnC_Cy

Cuts at:      0     642    1152    2127 
   Size:        642     510     975

  Fragments arranged by size: 

                 975     642     510
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     525     929    2127 
   Size:        525     404    1198

  Fragments arranged by size: 

                1198     525     404
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     265     297     490     522    1240    1272    1335    1354 
   Size:        265      32     193      32     718      32      63      19

Cuts at:   1354    1367    1386    1430    1462    1774    1806    2055    2087 
   Size:         13      19      44      32     312      32     249      32

Cuts at:   2087    2127 
   Size:         40

  Fragments arranged by size: 

                 718     312     265     249     193      63      44      40
                  32      32      32      32      32      32      19      19
                  13
 
HincII GTy'rAC

Cuts at:      0    1503    2127 
   Size:       1503     624
 
HindIII A'AGCT_T

Cuts at:      0     164    2127 
   Size:        164    1963
 
KpnI G_GTAC'C

Cuts at:      0    1576    2127 
   Size:       1576     551
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     432    1062    2127 
   Size:        432     630    1065

  Fragments arranged by size: 

                1065     630     432
 
MscI TGG'CCA

Cuts at:      0    1015    2127 
   Size:       1015    1112
 
MslI CAynn'nnrTG

Cuts at:      0     905    1021    2127 
   Size:        905     116    1106

  Fragments arranged by size: 

                1106     905     116
 
MspA1I CmG'CkG

Cuts at:      0      95     472     793    2127 
   Size:         95     377     321    1334

  Fragments arranged by size: 

                1334     377     321      95
 
PfoI T'CCnGG_A

Cuts at:      0     248    1181    2127 
   Size:        248     933     946

  Fragments arranged by size: 

                 946     933     248
 
PmlI CAC'GTG

Cuts at:      0    1940    2127 
   Size:       1940     187
 
PpuMI rG'GwC_Cy

Cuts at:      0    1152    2127 
   Size:       1152     975
 
PstI C_TGCA'G

Cuts at:      0    1070    2127 
   Size:       1070    1057
 
PvuII CAG'CTG

Cuts at:      0     472     793    2127 
   Size:        472     321    1334

  Fragments arranged by size: 

                1334     472     321
 
SacI G_AGCT'C

Cuts at:      0     269    2127 
   Size:        269    1858
 
SacII CC_GC'GG

Cuts at:      0      96    2127 
   Size:         96    2031
 
SfcI C'TryA_G

Cuts at:      0    1066    2127 
   Size:       1066    1061
 
SmlI C'TyrA_G

Cuts at:      0     181    2127 
   Size:        181    1946
 
SspI AAT'ATT

Cuts at:      0     948    2127 
   Size:        948    1179
 
StyI C'CwwG_G

Cuts at:      0      14     992    1568    2127 
   Size:         14     978     576     559

  Fragments arranged by size: 

                 978     576     559      14
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     839    1265    1351    2127 
   Size:        839     426      86     776

  Fragments arranged by size: 

                 839     776     426      86
 
TatI w'GTAC_w

Cuts at:      0     885    2127 
   Size:        885    1242
 
Tth111I GACn'n_nGTC

Cuts at:      0     849    1173    2127 
   Size:        849     324     954

  Fragments arranged by size: 

                 954     849     324
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1145    2127 
   Size:       1145     982
 
XmnI GAAnn'nnTTC

Cuts at:      0    1872    2127 
   Size:       1872     255
 
ZraI GAC'GTC

Cuts at:      0     516    1703    2127 
   Size:        516    1187     424

  Fragments arranged by size: 

                1187     516     424
 

 Enzymes that do cut:

    AatII   Acc65I     AcuI    AflII   AflIII     AhdI    AlwNI    ApaLI
     ApoI     AseI     BanI    BanII     BbsI    BbvCI     BcgI    BciVI
     BclI    BglII Bme1580I     BplI     BpmI   Bpu10I    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspMI    BsrDI    BssSI    BstBI    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     EaeI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI    Hin4I   HincII  HindIII     KpnI     MmeI     MscI
     MslI   MspA1I     PfoI     PmlI    PpuMI     PstI    PvuII     SacI
    SacII     SfcI     SmlI     SspI     StyI    TaqII     TatI  Tth111I
     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI     AclI     AfeI     AgeI     AleI     AlfI     AloI
     ApaI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI    BpuEI     BsaI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI
    BsrGI   BssHII   BstAPI   BstEII    BstXI  BstZ17I   Bsu36I     DrdI
     EagI     EarI     EciI    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII     HpaI     KasI     MfeI     MluI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI     TstI     XbaI     XhoI
     XmaI
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