Restriction map for pF1KA1887
 (Linear) (Six-base) MAPSORT of: KIAA1887.seq  Check: 541  from: 1  to: 3009



KIAA1887.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     367     660     896    3009 
   Size:        367     293     236    2113

  Fragments arranged by size: 

                2113     367     293     236
 
AccI GT'mk_AC

Cuts at:      0    2436    3009 
   Size:       2436     573
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     749     857    1796    1826    2780    3009 
   Size:        749     108     939      30     954     229

  Fragments arranged by size: 

                 954     939     749     229     108      30
 
AfeI AGC'GCT

Cuts at:      0      79    3009 
   Size:         79    2930
 
AflII C'TTAA_G

Cuts at:      0    2815    3009 
   Size:       2815     194
 
AflIII A'CryG_T

Cuts at:      0    2683    3009 
   Size:       2683     326
 
AleI CACnn'nnGTG

Cuts at:      0     788    3009 
   Size:        788    2221
 
AlwNI CAG_nnn'CTG

Cuts at:      0     541    1578    2476    2536    3009 
   Size:        541    1037     898      60     473

  Fragments arranged by size: 

                1037     898     541     473      60
 
ApaI G_GGCC'C

Cuts at:      0      46     425     908     921    1173    1897    2801    2964 
   Size:         46     379     483      13     252     724     904     163

Cuts at:   2964    3009 
   Size:         45

  Fragments arranged by size: 

                 904     724     483     379     252     163      46      45
                  13
 
ApoI r'AATT_y

Cuts at:      0    2028    3009 
   Size:       2028     981
 
AseI AT'TA_AT

Cuts at:      0    2603    3009 
   Size:       2603     406
 
AvaI C'yCGr_G

Cuts at:      0     249     819    1166    2064    2148    2443    2624    2794 
   Size:        249     570     347     898      84     295     181     170

Cuts at:   2794    2826    2933    2957    3009 
   Size:         32     107      24      52

  Fragments arranged by size: 

                 898     570     347     295     249     181     170     107
                  84      52      32      24
 
BamHI G'GATC_C

Cuts at:      0    2055    2781    3009 
   Size:       2055     726     228

  Fragments arranged by size: 

                2055     726     228
 
BanI G'GyrC_C

Cuts at:      0     711     942    1047    1361    1420    2221    2820    3009 
   Size:        711     231     105     314      59     801     599     189

  Fragments arranged by size: 

                 801     711     599     314     231     189     105      59
            
 
BanII G_rGCy'C

Cuts at:      0      46     394     425     730     908     921    1173    1348 
   Size:         46     348      31     305     178      13     252     175

Cuts at:   1348    1724    1897    2271    2349    2452    2777    2801    2964 
   Size:        376     173     374      78     103     325      24     163

Cuts at:   2964    3009 
   Size:         45

  Fragments arranged by size: 

                 376     374     348     325     305     252     178     175
                 173     163     103      78      46      45      31      24
                  13
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     125     863    2237    3009 
   Size:        125     738    1374     772

  Fragments arranged by size: 

                1374     772     738     125
 
BbvCI CC'TCA_GC

Cuts at:      0     163    1053    1405    2457    3009 
   Size:        163     890     352    1052     552

  Fragments arranged by size: 

                1052     890     552     352     163
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2434    2468    3009 
   Size:       2434      34     541

  Fragments arranged by size: 

                2434     541      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1779    2481    3009 
   Size:       1779     702     528

  Fragments arranged by size: 

                1779     702     528
 
BlpI GC'TnA_GC

Cuts at:      0    2771    3009 
   Size:       2771     238
 
Bme1580I G_kGCm'C

Cuts at:      0      46     425     716     908     921     945    1044    1173 
   Size:         46     379     291     192      13      24      99     129

Cuts at:   1173    1425    1897    2100    2801    2825    2964    3009 
   Size:        252     472     203     701      24     139      45

  Fragments arranged by size: 

                 701     472     379     291     252     203     192     139
                 129      99      46      45      24      24      13
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     568     693    1447    1504    1703    2524    2558    3009 
   Size:        568     125     754      57     199     821      34     451

  Fragments arranged by size: 

                 821     754     568     451     199     125      57      34
            
 
BmtI G_CTAG'C

Cuts at:      0     962    3009 
   Size:        962    2047
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     712    1157    1181    3009 
   Size:        712     445      24    1828

  Fragments arranged by size: 

                1828     712     445      24
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211     398     617    2106    2285    2386    2396    3009 
   Size:        211     187     219    1489     179     101      10     613

  Fragments arranged by size: 

                1489     613     219     211     187     179     101      10
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     163    1053    1405    1568    1718    2206    2457    2499 
   Size:        163     890     352     163     150     488     251      42

Cuts at:   2499    2537    3009 
   Size:         38     472

  Fragments arranged by size: 

                 890     488     472     352     251     163     163     150
                  42      38
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     346    1964    2184    3009 
   Size:        346    1618     220     825

  Fragments arranged by size: 

                1618     825     346     220
 
BsaAI yAC'GTr

Cuts at:      0    2686    3009 
   Size:       2686     323
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     792     822    1208    1238    2384    2414    3009 
   Size:        792      30     386      30    1146      30     595

  Fragments arranged by size: 

                1146     792     595     386      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     151     295     635     722     741     744     764    1056 
   Size:        151     144     340      87      19       3      20     292

Cuts at:   1056    1310    1326    1715    2148    2320    2339    2597    3006 
   Size:        254      16     389     433     172      19     258     409

Cuts at:   3006    3009 
   Size:          3

  Fragments arranged by size: 

                 433     409     389     340     292     258     254     172
                 151     144      87      20      19      19      16       3
                   3
 
BseYI C'CCAG_C

Cuts at:      0      37     259    1012    1245    1352    1462    1561    1852 
   Size:         37     222     753     233     107     110      99     291

Cuts at:   1852    1903    2078    2217    2232    2275    2920    3009 
   Size:         51     175     139      15      43     645      89

  Fragments arranged by size: 

                 753     645     291     233     222     175     139     110
                 107      99      89      51      43      37      15
 
BsiHKAI G_wGCw'C

Cuts at:      0     104    2349    3009 
   Size:        104    2245     660

  Fragments arranged by size: 

                2245     660     104
 
Bsp1286I G_dGCh'C

Cuts at:      0      46     104     394     425     716     730     908     921 
   Size:         46      58     290      31     291      14     178      13

Cuts at:    921     945    1044    1173    1348    1425    1724    1897    2100 
   Size:         24      99     129     175      77     299     173     203

Cuts at:   2100    2271    2349    2452    2777    2801    2825    2964    3009 
   Size:        171      78     103     325      24      24     139      45

  Fragments arranged by size: 

                 325     299     291     290     203     178     175     173
                 171     139     129     103      99      78      77      58
                  46      45      31      24      24      24      14      13
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     184     367     660     896    3009 
   Size:        184     183     293     236    2113

  Fragments arranged by size: 

                2113     293     236     184     183
 
BsrDI GCAATG_nn'

Cuts at:      0      18     627    1660    3009 
   Size:         18     609    1033    1349

  Fragments arranged by size: 

                1349    1033     609      18
 
BsrFI r'CCGG_y

Cuts at:      0     308    2645    3009 
   Size:        308    2337     364

  Fragments arranged by size: 

                2337     364     308
 
BstEII G'GTnAC_C

Cuts at:      0     243    2236    3009 
   Size:        243    1993     773

  Fragments arranged by size: 

                1993     773     243
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1279    2232    3009 
   Size:       1279     953     777

  Fragments arranged by size: 

                1279     953     777
 
BstYI r'GATC_y

Cuts at:      0     700    1918    2055    2405    2781    3009 
   Size:        700    1218     137     350     376     228

  Fragments arranged by size: 

                1218     700     376     350     228     137
 
Bsu36I CC'TnA_GG

Cuts at:      0    1338    3009 
   Size:       1338    1671
 
BtgI C'CryG_G

Cuts at:      0     923    2132    2511    3009 
   Size:        923    1209     379     498

  Fragments arranged by size: 

                1209     923     498     379
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     182    3009 
   Size:        182    2827
 
BtsI GCAGTG_nn'

Cuts at:      0      27    1416    3009 
   Size:         27    1389    1593

  Fragments arranged by size: 

                1593    1389      27
 
DraI TTT'AAA

Cuts at:      0    2027    3009 
   Size:       2027     982
 
DraIII CAC_nnn'GTG

Cuts at:      0     411    1588    2689    3009 
   Size:        411    1177    1101     320

  Fragments arranged by size: 

                1177    1101     411     320
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1160    2530    3009 
   Size:       1160    1370     479

  Fragments arranged by size: 

                1370    1160     479
 
EaeI y'GGCC_r

Cuts at:      0      50     980    2310    2658    2853    2970    3009 
   Size:         50     930    1330     348     195     117      39

  Fragments arranged by size: 

                1330     930     348     195     117      50      39
 
EarI CTCTTCn'nnn_

Cuts at:      0     375     784    2306    3009 
   Size:        375     409    1522     703

  Fragments arranged by size: 

                1522     703     409     375
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     327    3009 
   Size:        327    2682
 
EcoICRI GAG'CTC

Cuts at:      0    2347    3009 
   Size:       2347     662
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211     398     617     749     857    1796    1826    2106 
   Size:        211     187     219     132     108     939      30     280

Cuts at:   2106    2285    2386    2396    2780    3009 
   Size:        179     101      10     384     229

  Fragments arranged by size: 

                 939     384     280     229     219     211     187     179
                 132     108     101      30      10
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2362    3009 
   Size:       2362     647
 
EcoO109I rG'GnC_Cy

Cuts at:      0      42      43     173     421     422     478     538     904 
   Size:         42       1     130     248       1      56      60     366

Cuts at:    904     905     917     918     934    1034    1138    1170    1575 
   Size:          1      12       1      16     100     104      32     405

Cuts at:   1575    1616    1744    1780    1894    2153    2172    2714    2798 
   Size:         41     128      36     114     259      19     542      84

Cuts at:   2798    2811    3009 
   Size:         13     198

  Fragments arranged by size: 

                 542     405     366     259     248     198     130     128
                 114     104     100      84      60      56      42      41
                  36      32      19      16      13      12       1       1
                   1       1
 
HaeII r_GCGC'y

Cuts at:      0      81    3009 
   Size:         81    2928
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     712     744     813     845    2047    2079    2654    2686 
   Size:        712      32      69      32    1202      32     575      32

Cuts at:   2686    2927    2959    3009 
   Size:        241      32      50

  Fragments arranged by size: 

                1202     712     575     241      69      50      32      32
                  32      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1284    3009 
   Size:       1284    1725
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2204    3009 
   Size:       2204     805
 
MscI TGG'CCA

Cuts at:      0      52    2312    3009 
   Size:         52    2260     697

  Fragments arranged by size: 

                2260     697      52
 
MslI CAynn'nnrTG

Cuts at:      0     788    1277    3009 
   Size:        788     489    1732

  Fragments arranged by size: 

                1732     788     489
 
MspA1I CmG'CkG

Cuts at:      0     979    1438    1903    2941    3009 
   Size:        979     459     465    1038      68

  Fragments arranged by size: 

                1038     979     465     459      68
 
NaeI GCC'GGC

Cuts at:      0    2647    3009 
   Size:       2647     362
 
NcoI C'CATG_G

Cuts at:      0    2511    3009 
   Size:       2511     498
 
NgoMIV G'CCGG_C

Cuts at:      0    2645    3009 
   Size:       2645     364
 
NheI G'CTAG_C

Cuts at:      0     958    3009 
   Size:        958    2051
 
PasI CC'CwG_GG

Cuts at:      0     418     541     909    1603    2335    3009 
   Size:        418     123     368     694     732     674

  Fragments arranged by size: 

                 732     694     674     418     368     123
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      71     472    2512    3009 
   Size:         71     401    2040     497

  Fragments arranged by size: 

                2040     497     401      71
 
PfoI T'CCnGG_A

Cuts at:      0     375    1202    3009 
   Size:        375     827    1807

  Fragments arranged by size: 

                1807     827     375
 
PmlI CAC'GTG

Cuts at:      0    2686    3009 
   Size:       2686     323
 
PpuMI rG'GwC_Cy

Cuts at:      0     173     478    1744    2714    2811    3009 
   Size:        173     305    1266     970      97     198

  Fragments arranged by size: 

                1266     970     305     198     173      97
 
PspOMI G'GGCC_C

Cuts at:      0      42     421     904     917    1169    1893    2797    2960 
   Size:         42     379     483      13     252     724     904     163

Cuts at:   2960    3009 
   Size:         49

  Fragments arranged by size: 

                 904     724     483     379     252     163      49      42
                  13
 
PstI C_TGCA'G

Cuts at:      0     978    1926    2327    3009 
   Size:        978     948     401     682

  Fragments arranged by size: 

                 978     948     682     401
 
PvuII CAG'CTG

Cuts at:      0    1438    1903    2941    3009 
   Size:       1438     465    1038      68

  Fragments arranged by size: 

                1438    1038     465      68
 
SacI G_AGCT'C

Cuts at:      0    2349    3009 
   Size:       2349     660
 
SexAI A'CCwGG_T

Cuts at:      0    1746    3009 
   Size:       1746    1263
 
SfcI C'TryA_G

Cuts at:      0     786     974    1922    2323    2392    2927    3009 
   Size:        786     188     948     401      69     535      82

  Fragments arranged by size: 

                 948     786     535     401     188      82      69
 
SgrAI Cr'CCGG_yG

Cuts at:      0     308    3009 
   Size:        308    2701
 
SmaI CCC'GGG

Cuts at:      0     251    2150    2626    2796    2828    2959    3009 
   Size:        251    1899     476     170      32     131      50

  Fragments arranged by size: 

                1899     476     251     170     131      50      32
 
SmlI C'TyrA_G

Cuts at:      0    2815    3009 
   Size:       2815     194
 
StuI AGG'CCT

Cuts at:      0     520    2484    3009 
   Size:        520    1964     525

  Fragments arranged by size: 

                1964     525     520
 
StyI C'CwwG_G

Cuts at:      0     137     212     559     613     983    2511    2777    2912 
   Size:        137      75     347      54     370    1528     266     135

Cuts at:   2912    3009 
   Size:         97

  Fragments arranged by size: 

                1528     370     347     266     137     135      97      75
                  54
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2209    2224    3009 
   Size:       2209      15     785

  Fragments arranged by size: 

                2209     785      15
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     191     223     397     429     935     967    1380    1412 
   Size:        191      32     174      32     506      32     413      32

Cuts at:   1412    2040    2072    2673    2705    3009 
   Size:        628      32     601      32     304

  Fragments arranged by size: 

                 628     601     506     413     304     191     174      32
                  32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     574    1748    3009 
   Size:        574    1174    1261

  Fragments arranged by size: 

                1261    1174     574
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1678    3009 
   Size:       1678    1331
 
XmaI C'CCGG_G

Cuts at:      0     249    2148    2624    2794    2826    2957    3009 
   Size:        249    1899     476     170      32     131      52

  Fragments arranged by size: 

                1899     476     249     170     131      52      32
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI    AflII   AflIII     AleI    AlwNI
     ApaI     ApoI     AseI     AvaI    BamHI     BanI    BanII     BbsI
    BbvCI     BcgI     BglI     BlpI Bme1580I     BmrI     BmtI     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BsaXI    BseRI    BseYI  BsiHKAI
 Bsp1286I    BspMI    BsrDI    BsrFI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I  HindIII
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NheI
     PasI    PflMI     PfoI     PmlI    PpuMI   PspOMI     PstI    PvuII
     SacI    SexAI     SfcI    SgrAI     SmaI     SmlI     StuI     StyI
    TaqII     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AgeI     AhdI     AlfI     AloI    ApaLI
     AscI    AsiSI    AvrII     BaeI     BbeI    BciVI     BclI    BfrBI
    BglII    BmgBI    BpuEI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrGI   BssHII
    BssSI   BstAPI    BstBI  BstZ17I     ClaI    CspCI     EagI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII     HpaI     KasI
     KpnI     MfeI     MluI     NarI     NdeI     NotI     NruI     NsiI
     NspI     PacI     PciI     PmeI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI     SfiI     SfoI     SgfI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TatI     XbaI     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com