(Circular) (Six-base) MAPSORT of: pF1KA0806.seq Check: 485 from: 1 to: 6308 pF1KA0806 6308 bp With 182 enzymes: SgfI * April 24, 2009 13:00 .. AatII G_ACGT'C Cuts at: 2084 2084 Size: 6308 AccI GT'mk_AC Cuts at: 3346 3346 Size: 6308 Acc65I G'GTAC_C Cuts at: 2797 3324 2797 Size: 527 5781 AclI AA'CG_TT Cuts at: 6148 6148 Size: 6308 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1174 4133 4565 4918 5580 1174 Size: 2959 432 353 662 1902 Fragments arranged by size: 2959 1902 662 432 353 AflII C'TTAA_G Cuts at: 828 868 1203 828 Size: 40 335 5933 Fragments arranged by size: 5933 335 40 AflIII A'CryG_T Cuts at: 3660 5460 5741 3660 Size: 1800 281 4227 Fragments arranged by size: 4227 1800 281 AleI CACnn'nnGTG Cuts at: 1669 1669 Size: 6308 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 249 304 249 Size: 55 6253 AlwNI CAG_nnn'CTG Cuts at: 147 2907 3613 5051 147 Size: 2760 706 1438 1404 Fragments arranged by size: 2760 1438 1404 706 ApaLI G'TGCA_C Cuts at: 1520 1662 5146 1520 Size: 142 3484 2682 Fragments arranged by size: 3484 2682 142 ApoI r'AATT_y Cuts at: 624 1128 1217 1746 1818 3312 5661 5917 624 Size: 504 89 529 72 1494 2349 256 1015 Fragments arranged by size: 2349 1494 1015 529 504 256 89 72 AseI AT'TA_AT Cuts at: 20 20 Size: 6308 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6308 AvaI C'yCGr_G Cuts at: 2045 2071 2231 2801 2852 3328 2045 Size: 26 160 570 51 476 5025 Fragments arranged by size: 5025 570 476 160 51 26 AvrII C'CTAG_G Cuts at: 123 123 Size: 6308 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2607 2640 2789 2822 2607 Size: 33 149 33 6093 Fragments arranged by size: 6093 149 33 33 BamHI G'GATC_C Cuts at: 3333 3333 Size: 6308 BanI G'GyrC_C Cuts at: 111 117 1954 2797 3324 3738 3986 4021 111 Size: 6 1837 843 527 414 248 35 2398 Fragments arranged by size: 2398 1837 843 527 414 248 35 6 BanII G_rGCy'C Cuts at: 2645 3322 4352 2645 Size: 677 1030 4601 Fragments arranged by size: 4601 1030 677 BbeI G_GCGC'C Cuts at: 115 121 3742 3990 115 Size: 6 3621 248 2433 Fragments arranged by size: 3621 2433 248 6 BbsI GAAGACnn'nnnn_ Cuts at: 2076 2621 2669 2076 Size: 545 48 5715 Fragments arranged by size: 5715 545 48 BbvCI CC'TCA_GC Cuts at: 559 2882 559 Size: 2323 3985 BciVI GTATCCnnnnn_n' Cuts at: 1731 2958 4206 5262 6308 1731 Size: 1227 1248 1056 1046 1731 Fragments arranged by size: 1731 1248 1227 1056 1046 BclI T'GATC_A Cuts at: 2898 3828 2898 Size: 930 5378 BfrBI ATG'CAT Cuts at: 1024 1024 Size: 6308 BglII A'GATC_T Cuts at: 737 832 3823 5860 737 Size: 95 2991 2037 1185 Fragments arranged by size: 2991 2037 1185 95 BlpI GC'TnA_GC Cuts at: 3420 3420 Size: 6308 Bme1580I G_kGCm'C Cuts at: 1524 1666 3301 3933 4026 5150 1524 Size: 142 1635 632 93 1124 2682 Fragments arranged by size: 2682 1635 1124 632 142 93 BmgBI CAC'GTC Cuts at: 1661 1734 2064 1661 Size: 73 330 5905 Fragments arranged by size: 5905 330 73 BmrI ACTGGGnnnn_n' Cuts at: 3520 3601 3936 6080 3520 Size: 81 335 2144 3748 Fragments arranged by size: 3748 2144 335 81 BmtI G_CTAG'C Cuts at: 1540 1540 Size: 6308 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 247 2211 4675 247 Size: 1964 2464 1880 Fragments arranged by size: 2464 1964 1880 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 324 511 565 1793 2233 2285 2829 324 Size: 187 54 1228 440 52 544 3803 Fragments arranged by size: 3803 1228 544 440 187 54 52 Bpu10I CC'TnA_GC Cuts at: 559 2882 559 Size: 2323 3985 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1363 1579 3484 4363 5077 5375 1363 Size: 216 1905 879 714 298 2296 Fragments arranged by size: 2296 1905 879 714 298 216 BsaI GGTCTCn'nnnn_ Cuts at: 235 2161 6012 235 Size: 1926 3851 531 Fragments arranged by size: 3851 1926 531 BsaAI yAC'GTr Cuts at: 3661 3661 Size: 6308 BsaBI GATnn'nnATC Cuts at: 6 3822 5859 6 Size: 3816 2037 455 Fragments arranged by size: 3816 2037 455 BsaHI Gr'CG_yC Cuts at: 112 118 128 2081 3739 3987 4689 6185 112 Size: 6 10 1953 1658 248 702 1496 235 Fragments arranged by size: 1953 1658 1496 702 248 235 10 6 BsaWI w'CCGG_w Cuts at: 3497 4018 4696 5107 5254 5528 3497 Size: 521 678 411 147 274 4277 Fragments arranged by size: 4277 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 127 157 509 539 2827 2857 127 Size: 30 352 30 2288 30 3578 Fragments arranged by size: 3578 2288 352 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 151 193 2653 2899 151 Size: 42 2460 246 3560 Fragments arranged by size: 3560 2460 246 42 BseYI C'CCAG_C Cuts at: 147 2389 2665 3143 3733 4491 5156 5564 147 Size: 2242 276 478 590 758 665 408 891 Fragments arranged by size: 2242 891 758 665 590 478 408 276 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1979 1979 Size: 6308 BsiEI CG_ry'CG Cuts at: 104 3850 3896 5126 104 Size: 3746 46 1230 1286 Fragments arranged by size: 3746 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 1524 1666 2645 3322 4100 4290 5150 1524 Size: 142 979 677 778 190 860 2682 Fragments arranged by size: 2682 979 860 778 677 190 142 BsmI GAATG_Cn' Cuts at: 257 1002 1321 257 Size: 745 319 5244 Fragments arranged by size: 5244 745 319 BsmBI CGTCTCn'nnnn_ Cuts at: 2290 2290 Size: 6308 Bsp1286I G_dGCh'C Cuts at: 1524 1666 2645 3301 3322 3933 4026 4100 4290 Size: 142 979 656 21 611 93 74 190 Cuts at: 4290 4352 5150 1524 Size: 62 798 2682 Fragments arranged by size: 2682 979 798 656 611 190 142 93 74 62 21 BspHI T'CATG_A Cuts at: 4740 4740 Size: 6308 BspMI ACCTGCnnnn'nnnn_ Cuts at: 217 2410 3358 3572 3874 4255 217 Size: 2193 948 214 302 381 2270 Fragments arranged by size: 2270 2193 948 381 302 214 BsrBI CCG'CTC Cuts at: 50 199 3074 4600 4654 6138 6303 50 Size: 149 2875 1526 54 1484 165 55 Fragments arranged by size: 2875 1526 1484 165 149 55 54 BsrDI GCAATG_nn' Cuts at: 185 3082 4220 185 Size: 2897 1138 2273 Fragments arranged by size: 2897 2273 1138 BsrFI r'CCGG_y Cuts at: 114 4306 4487 114 Size: 4192 181 1935 Fragments arranged by size: 4192 1935 181 BssSI C'ACGA_G Cuts at: 935 4579 5287 935 Size: 3644 708 1956 Fragments arranged by size: 3644 1956 708 BstAPI GCAn_nnn'nTGC Cuts at: 2067 2748 3685 2067 Size: 681 937 4690 Fragments arranged by size: 4690 937 681 BstBI TT'CG_AA Cuts at: 4669 4669 Size: 6308 BstXI CCAn_nnnn'nTGG Cuts at: 2919 2919 Size: 6308 BstYI r'GATC_y Cuts at: 1 737 832 1563 1935 3333 3800 3823 4158 Size: 736 95 731 372 1398 467 23 335 Cuts at: 4158 4404 4710 4722 4808 5860 1 Size: 246 306 12 86 1052 449 Fragments arranged by size: 1398 1052 736 731 467 449 372 335 306 246 95 86 23 12 BtgI C'CryG_G Cuts at: 107 1958 107 Size: 1851 4457 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 4258 4439 4602 4258 Size: 181 163 5964 Fragments arranged by size: 5964 181 163 BtsI GCAGTG_nn' Cuts at: 1664 1676 1686 3551 1664 Size: 12 10 1865 4421 Fragments arranged by size: 4421 1865 12 10 DraI TTT'AAA Cuts at: 1412 3307 1412 Size: 1895 4413 DraIII CAC_nnn'GTG Cuts at: 1703 1703 Size: 6308 DrdI GACnn_nn'nnGTC Cuts at: 1144 1265 4014 5358 1144 Size: 121 2749 1344 2094 Fragments arranged by size: 2749 2094 1344 121 EaeI y'GGCC_r Cuts at: 1961 2436 3893 4067 4458 4485 5830 1961 Size: 475 1457 174 391 27 1345 2439 Fragments arranged by size: 2439 1457 1345 475 391 174 27 EagI C'GGCC_G Cuts at: 3893 3893 Size: 6308 EarI CTCTTCn'nnn_ Cuts at: 187 2484 4331 4541 187 Size: 2297 1847 210 1954 Fragments arranged by size: 2297 1954 1847 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 4516 5258 5404 6118 4516 Size: 742 146 714 4706 Fragments arranged by size: 4706 742 714 146 EcoICRI GAG'CTC Cuts at: 2643 3320 2643 Size: 677 5631 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 324 511 565 1174 1793 2233 2285 2829 4133 Size: 187 54 609 619 440 52 544 1304 Cuts at: 4133 4565 4918 5580 324 Size: 432 353 662 1052 Fragments arranged by size: 1304 1052 662 619 609 544 440 432 353 187 54 52 EcoNI CCTnn'n_nnAGG Cuts at: 3234 3234 Size: 6308 EcoO109I rG'GnC_Cy Cuts at: 2849 3186 3447 2849 Size: 337 261 5710 Fragments arranged by size: 5710 337 261 EcoRI G'AATT_C Cuts at: 3312 3312 Size: 6308 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4327 4327 Size: 6308 FspI TGC'GCA Cuts at: 4089 4089 Size: 6308 HaeII r_GCGC'y Cuts at: 115 121 313 3742 3990 5220 5991 115 Size: 6 192 3429 248 1230 771 432 Fragments arranged by size: 3429 1230 771 432 248 192 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 547 579 661 693 1897 1929 4150 4180 4182 Size: 32 82 32 1204 32 2221 30 2 Cuts at: 4182 4212 547 Size: 30 2643 Fragments arranged by size: 2643 2221 1204 82 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 3347 3347 Size: 6308 KasI G'GCGC_C Cuts at: 111 117 3738 3986 111 Size: 6 3621 248 2433 Fragments arranged by size: 3621 2433 248 6 KpnI G_GTAC'C Cuts at: 2801 3328 2801 Size: 527 5781 MfeI C'AATT_G Cuts at: 2469 2469 Size: 6308 MluI A'CGCG_T Cuts at: 5741 5741 Size: 6308 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 5067 5251 5067 Size: 184 6124 MscI TGG'CCA Cuts at: 1963 2438 4069 1963 Size: 475 1631 4202 Fragments arranged by size: 4202 1631 475 MslI CAynn'nnrTG Cuts at: 1648 1669 2504 2542 3067 3179 4297 4424 1648 Size: 21 835 38 525 112 1118 127 3532 Fragments arranged by size: 3532 1118 835 525 127 112 38 21 MspA1I CmG'CkG Cuts at: 849 1074 3090 3419 3733 4093 4877 5122 849 Size: 225 2016 329 314 360 784 245 2035 Fragments arranged by size: 2035 2016 784 360 329 314 245 225 NaeI GCC'GGC Cuts at: 116 4489 116 Size: 4373 1935 NarI GG'CG_CC Cuts at: 112 118 3739 3987 112 Size: 6 3621 248 2433 Fragments arranged by size: 3621 2433 248 6 NcoI C'CATG_G Cuts at: 107 1958 107 Size: 1851 4457 NgoMIV G'CCGG_C Cuts at: 114 4487 114 Size: 4373 1935 NheI G'CTAG_C Cuts at: 1536 1536 Size: 6308 NsiI A_TGCA'T Cuts at: 1026 1026 Size: 6308 NspI r_CATG'y Cuts at: 609 2061 3361 3861 5464 609 Size: 1452 1300 500 1603 1453 Fragments arranged by size: 1603 1453 1452 1300 500 PciI A'CATG_T Cuts at: 5460 5460 Size: 6308 PflMI CCAn_nnn'nTGG Cuts at: 765 1703 765 Size: 938 5370 PfoI T'CCnGG_A Cuts at: 131 266 344 2845 131 Size: 135 78 2501 3594 Fragments arranged by size: 3594 2501 135 78 PmeI GTTT'AAAC Cuts at: 3307 3307 Size: 6308 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4754 4786 4754 Size: 32 6276 PpuMI rG'GwC_Cy Cuts at: 2849 2849 Size: 6308 PshAI GACnn'nnGTC Cuts at: 275 275 Size: 6308 PstI C_TGCA'G Cuts at: 277 1671 1983 2346 2658 3355 4040 277 Size: 1394 312 363 312 697 685 2545 Fragments arranged by size: 2545 1394 697 685 363 312 312 PvuI CG_AT'CG Cuts at: 104 104 Size: 6308 PvuII CAG'CTG Cuts at: 1074 3733 4093 1074 Size: 2659 360 3289 Fragments arranged by size: 3289 2659 360 RsrII CG'GwC_CG Cuts at: 4503 4503 Size: 6308 SacI G_AGCT'C Cuts at: 2645 3322 2645 Size: 677 5631 SalI G'TCGA_C Cuts at: 3345 3345 Size: 6308 SapI GCTCTTCn'nnn_ Cuts at: 4331 4541 4331 Size: 210 6098 SbfI CC_TGCA'GG Cuts at: 3355 3355 Size: 6308 ScaI AGT'ACT Cuts at: 5825 5825 Size: 6308 SfcI C'TryA_G Cuts at: 33 273 1667 1979 2124 2342 2619 2654 2712 Size: 240 1394 312 145 218 277 35 58 Cuts at: 2712 3351 4036 5004 5195 33 Size: 639 685 968 191 1146 Fragments arranged by size: 1394 1146 968 685 639 312 277 240 218 191 145 58 35 SfoI GGC'GCC Cuts at: 113 119 3740 3988 113 Size: 6 3621 248 2433 Fragments arranged by size: 3621 2433 248 6 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6308 SgrAI Cr'CCGG_yG Cuts at: 114 114 Size: 6308 SmaI CCC'GGG Cuts at: 2803 3330 2803 Size: 527 5781 SmlI C'TyrA_G Cuts at: 828 868 1203 1342 1594 2045 3463 4378 5092 Size: 40 335 139 252 451 1418 915 714 Cuts at: 5092 5354 828 Size: 262 1782 Fragments arranged by size: 1782 1418 915 714 451 335 262 252 139 40 SpeI A'CTAG_T Cuts at: 67 1792 67 Size: 1725 4583 SphI G_CATG'C Cuts at: 609 2061 3361 3861 609 Size: 1452 1300 500 3056 Fragments arranged by size: 3056 1452 1300 500 SspI AAT'ATT Cuts at: 471 471 Size: 6308 StuI AGG'CCT Cuts at: 3122 3122 Size: 6308 StyI C'CwwG_G Cuts at: 107 123 650 813 1958 2421 2682 3442 3796 Size: 16 527 163 1145 463 261 760 354 Cuts at: 3796 107 Size: 2619 Fragments arranged by size: 2619 1145 760 527 463 354 261 163 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1704 3891 4024 4483 4692 1704 Size: 2187 133 459 209 3320 Fragments arranged by size: 3320 2187 459 209 133 TatI w'GTAC_w Cuts at: 2949 5823 2949 Size: 2874 3434 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 509 541 2418 2450 5825 5857 509 Size: 32 1877 32 3375 32 960 Fragments arranged by size: 3375 1877 960 32 32 32 Tth111I GACn'n_nGTC Cuts at: 2907 4105 2907 Size: 1198 5110 XbaI T'CTAG_A Cuts at: 160 1350 3339 160 Size: 1190 1989 3129 Fragments arranged by size: 3129 1989 1190 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2491 3803 2491 Size: 1312 4996 XhoI C'TCGA_G Cuts at: 2045 2045 Size: 6308 XmaI C'CCGG_G Cuts at: 2801 3328 2801 Size: 527 5781 XmnI GAAnn'nnTTC Cuts at: 1321 1421 1321 Size: 100 6208 ZraI GAC'GTC Cuts at: 2082 2082 Size: 6308 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AgeI AhdI AloI ApaI AscI BcgI BglI BsiWI BspEI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI NdeI NotI NruI PacI PasI PmlI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SnaBI SrfI SwaI