Restriction map for pF1KE4227
##################################
# NO CUT ENZYMES (  100 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        Alw44I      AocI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspI        
AviII       AvrII       BamHI       BcgI        BciVI       
BfrI        BglII       BlnI        BsaBI       BsaI        
BseAI       BsiEI       BsmBI       BsmI        BspDI       
BspEI       BspHI       BspLU11I    BsrFI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      Cfr10I      ClaI        CpoI        DraI        
DraIII      EagI        EclXI       Eco52I      Eco81I      
EcoO65I     EcoT22I     FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        MamI        
MfeI        MluI        MroI        MunI        NaeI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SalI        ScaI        SexAI       SfuI        
SgrAI       SnaBI       SpeI        SphI        SspI        
SwaI        Tth111I     XbaI        XhoI        XmnI        


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# ONE CUT ENZYMES (   40 enzymes) #
###################################

AcsI          639  GAATTC               [GA]^AATT[CT]
AflIII       1997  ACGTGT               A^C[GA][CT]GT
Aor51HI      1031  AGCGCT               AGC^GCT
ApoI          639  GAATTC               [GA]^AATT[CT]
BbeI          848  GGCGCC               GGCGC^C
BbrPI        1996  CACGTG               CAC^GTG
BbvCI        2328  CCTCAGC              CCTCAGC(-5/-2)
BclI         1151  TGATCA               T^GATCA
BsaAI        1996  CACGTG               [CT]AC^GT[GA]
BsaWI         676  ACCGGA               [AT]^CCGG[AT]
BsiWI        1080  CGTACG               C^GTACG
Bsp1407I     1680  TGTACA               T^GTACA
BsrDI        1670  GCAATG               GCAATG(2/0)
BsrGI        1680  TGTACA               T^GTACA
BssSI         770  CACGAG               CACGAG(-5/-1)
BstAPI       1822  GCACCCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eco47III     1031  AGCGCT               AGC^GCT
EcoRI         639  GAATTC               G^AATTC
EcoRV        1069  GATATC               GAT^ATC
FbaI         1151  TGATCA               T^GATCA
KasI          848  GGCGCC               G^GCGCC
KspI          991  CCGCGG               CCGC^GG
MseI          869  TTAA                 T^TAA
NarI          848  GGCGCC               GG^CGCC
PflMI         754  CCAGCCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1996  CACGTG               CAC^GTG
PmlI         1996  CACGTG               CAC^GTG
PvuII         300  CAGCTG               CAG^CTG
SacII         991  CCGCGG               CCGC^GG
SapI          811  GAAGAGC              GCTCTTC(1/4)
SbfI         1302  CCTGCAGG             CCTGCA^GG
SfiI         1626  GGCCACCAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          848  GGCGCC               GGC^GCC
Sse8387I     1302  CCTGCAGG             CCTGCA^GG
SspBI        1680  TGTACA               T^GTACA
TfiI          860  GATTC                G^A[AT]TC
Tru9I         869  TTAA                 T^TAA
Tsp509I       640  AATT                 ^AATT
Van91I        754  CCAGCCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         2378  CCACCAGCAAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AccI         1393  GTCTAC               GT^[AC][GT]AC
             1858  GTCTAC              
AcyI          848  GGCGCC               G[GA]^CG[CT]C
             1093  GGCGTC              
AhdI          739  GACCGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2218  GACATCCTGTC         
ApaI          945  GGGCCC               GGGCC^C
             2054  GGGCCC              
AspEI         739  GACCGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2218  GACATCCTGTC         
BglI         1627  GCCACCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1936  GCCAAGATGGC         
BsaHI         848  GGCGCC               G[GA]^CG[CT]C
             1093  GGCGTC              
BsmAI         855  GTCTC                GTCTC(1/5)
             1497  GTCTC               
BstXI          33  CCACCTGACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2381  CCAGCAAACTGG        
BstYI        1727  AGATCC               [GA]^GATC[CT]
             2084  GGATCT              
DrdI          344  GACCCTGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2119  GACAAGGAGGTC        
Eam1105I      739  GACCGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2218  GACATCCTGTC         
HgaI         1094  GCGTC                GACGC(5/10)
             1512  GCGTC               
Hin1I         848  GGCGCC               G[GA]^CG[CT]C
             1093  GGCGTC              
MflI         1727  AGATCC               [GA]^GATC[CT]
             2084  GGATCT              
MslI          549  CACCCGAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2188  CACCCAGATG          
SmlI         1564  CTCAAG               C^T[CT][GA]AG
             1648  CTCAAG              
XhoII        1727  AGATCC               [GA]^GATC[CT]
             2084  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvI        BcnI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MluNI       
MnlI        MscI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        PpuMI       PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        StuI        StyI        TaqI        TseI        
Tsp45I      TspRI       TthHB8I     XmaCI       XmaI        
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