Restriction map for pF1KE4227
 (Linear) (Six-base) MAPSORT of: KIEE4227.seq  Check: 2450  from: 1  to: 2406



KIEE4227.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      24    1944    2406 
   Size:         24    1920     462

  Fragments arranged by size: 

                1920     462      24
 
AccI GT'mk_AC

Cuts at:      0    1394    1859    2406 
   Size:       1394     465     547

  Fragments arranged by size: 

                1394     547     465
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139    1329    1609    1631    1858    1993    2406 
   Size:        139    1190     280      22     227     135     413

  Fragments arranged by size: 

                1190     413     280     227     139     135      22
 
AfeI AGC'GCT

Cuts at:      0    1033    2406 
   Size:       1033    1373
 
AflIII A'CryG_T

Cuts at:      0    1997    2406 
   Size:       1997     409
 
AhdI GACnn_n'nnGTC

Cuts at:      0     744    2223    2406 
   Size:        744    1479     183

  Fragments arranged by size: 

                1479     744     183
 
AleI CACnn'nnGTG

Cuts at:      0     553    2406 
   Size:        553    1853
 
AlwNI CAG_nnn'CTG

Cuts at:      0     191     510     760    1257    1311    1323    1446    1515 
   Size:        191     319     250     497      54      12     123      69

Cuts at:   1515    2046    2406 
   Size:        531     360

  Fragments arranged by size: 

                 531     497     360     319     250     191     123      69
                  54      12
 
ApaI G_GGCC'C

Cuts at:      0     949    2058    2406 
   Size:        949    1109     348

  Fragments arranged by size: 

                1109     949     348
 
ApoI r'AATT_y

Cuts at:      0     639    2406 
   Size:        639    1767
 
AvaI C'yCGr_G

Cuts at:      0     126     457     551     650     686     948    1273    2406 
   Size:        126     331      94      99      36     262     325    1133

  Fragments arranged by size: 

                1133     331     325     262     126      99      94      36
            
 
BanI G'GyrC_C

Cuts at:      0     319     673     848    1806    2295    2406 
   Size:        319     354     175     958     489     111

  Fragments arranged by size: 

                 958     489     354     319     175     111
 
BanII G_rGCy'C

Cuts at:      0     133     180     527     794     949    1609    2058    2406 
   Size:        133      47     347     267     155     660     449     348

  Fragments arranged by size: 

                 660     449     348     347     267     155     133      47
            
 
BbeI G_GCGC'C

Cuts at:      0     852    2406 
   Size:        852    1554
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     465    1619    1625    2218    2406 
   Size:        465    1154       6     593     188

  Fragments arranged by size: 

                1154     593     465     188       6
 
BbvCI CC'TCA_GC

Cuts at:      0    2329    2406 
   Size:       2329      77
 
BclI T'GATC_A

Cuts at:      0    1151    2406 
   Size:       1151    1255
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1633    1942    2406 
   Size:       1633     309     464

  Fragments arranged by size: 

                1633     464     309
 
BlpI GC'TnA_GC

Cuts at:      0    1108    1222    1946    2406 
   Size:       1108     114     724     460

  Fragments arranged by size: 

                1108     724     460     114
 
Bme1580I G_kGCm'C

Cuts at:      0     324     657     949    2058    2101    2300    2406 
   Size:        324     333     292    1109      43     199     106

  Fragments arranged by size: 

                1109     333     324     292     199     106      43
 
BmgBI CAC'GTC

Cuts at:      0     900    1359    2406 
   Size:        900     459    1047

  Fragments arranged by size: 

                1047     900     459
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      50     127    2398    2406 
   Size:         50      77    2271       8

  Fragments arranged by size: 

                2271      77      50       8
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     577    2406 
   Size:        577    1829
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      10     358     369    1333    1345    1474    1510    1993 
   Size:         10     348      11     964      12     129      36     483

Cuts at:   1993    2088    2406 
   Size:         95     318

  Fragments arranged by size: 

                 964     483     348     318     129      95      36      12
                  11      10
 
Bpu10I CC'TnA_GC

Cuts at:      0     511     572    1717    1966    2329    2406 
   Size:        511      61    1145     249     363      77

  Fragments arranged by size: 

                1145     511     363     249      77      61
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1549    1633    2406 
   Size:       1549      84     773

  Fragments arranged by size: 

                1549     773      84
 
BsaAI yAC'GTr

Cuts at:      0    1998    2406 
   Size:       1998     408
 
BsaHI Gr'CG_yC

Cuts at:      0     849    1094    2406 
   Size:        849     245    1312

  Fragments arranged by size: 

                1312     849     245
 
BsaWI w'CCGG_w

Cuts at:      0     676    2406 
   Size:        676    1730
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      79     109     388     418    1126    1156    1781    1811 
   Size:         79      30     279      30     708      30     625      30

Cuts at:   1811    2406 
   Size:        595

  Fragments arranged by size: 

                 708     625     595     279      79      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     181     535     952     955     988    1507    1996    2005 
   Size:        181     354     417       3      33     519     489       9

Cuts at:   2005    2406 
   Size:        401

  Fragments arranged by size: 

                 519     489     417     401     354     181      33       9
                   3
 
BseYI C'CCAG_C

Cuts at:      0     286     323     330    1009    2406 
   Size:        286      37       7     679    1397

  Fragments arranged by size: 

                1397     679     286      37       7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     598    1681    2170    2406 
   Size:        598    1083     489     236

  Fragments arranged by size: 

                1083     598     489     236
 
BsiHKAI G_wGCw'C

Cuts at:      0     517     838    1288    2406 
   Size:        517     321     450    1118

  Fragments arranged by size: 

                1118     517     450     321
 
BsiWI C'GTAC_G

Cuts at:      0    1080    2406 
   Size:       1080    1326
 
Bsp1286I G_dGCh'C

Cuts at:      0     133     180     324     517     527     657     794     838 
   Size:        133      47     144     193      10     130     137      44

Cuts at:    838     949    1288    1609    2058    2101    2300    2406 
   Size:        111     339     321     449      43     199     106

  Fragments arranged by size: 

                 449     339     321     199     193     144     137     133
                 130     111     106      47      44      43      10
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      24    1406    1944    2406 
   Size:         24    1382     538     462

  Fragments arranged by size: 

                1382     538     462      24
 
BsrBI CCG'CTC

Cuts at:      0     576     734     846    2406 
   Size:        576     158     112    1560

  Fragments arranged by size: 

                1560     576     158     112
 
BsrDI GCAATG_nn'

Cuts at:      0    1677    2406 
   Size:       1677     729
 
BsrGI T'GTAC_A

Cuts at:      0    1680    2406 
   Size:       1680     726
 
BssSI C'ACGA_G

Cuts at:      0     770    2406 
   Size:        770    1636
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1828    2406 
   Size:       1828     578
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      40    2388    2406 
   Size:         40    2348      18

  Fragments arranged by size: 

                2348      40      18
 
BstYI r'GATC_y

Cuts at:      0    1727    2084    2406 
   Size:       1727     357     322

  Fragments arranged by size: 

                1727     357     322
 
BtgI C'CryG_G

Cuts at:      0     387     991    2019    2406 
   Size:        387     604    1028     387

  Fragments arranged by size: 

                1028     604     387     387
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2094    2406 
   Size:       2094     312
 
BtsI GCAGTG_nn'

Cuts at:      0      53      80    1258    1371    1810    2182    2406 
   Size:         53      27    1178     113     439     372     224

  Fragments arranged by size: 

                1178     439     372     224     113      53      27
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     350    2125    2406 
   Size:        350    1775     281

  Fragments arranged by size: 

                1775     350     281
 
EaeI y'GGCC_r

Cuts at:      0     232     304     486     751     902    1100    1625    1992 
   Size:        232      72     182     265     151     198     525     367

Cuts at:   1992    2406 
   Size:        414

  Fragments arranged by size: 

                 525     414     367     265     232     198     182     151
                  72
 
EarI CTCTTCn'nnn_

Cuts at:      0     434     624     806    1687    1876    2309    2406 
   Size:        434     190     182     881     189     433      97

  Fragments arranged by size: 

                 881     434     433     190     189     182      97
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     651    1914    2257    2406 
   Size:        651    1263     343     149

  Fragments arranged by size: 

                1263     651     343     149
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      10     139     358     369    1329    1333    1345    1474 
   Size:         10     129     219      11     960       4      12     129

Cuts at:   1474    1510    1609    1631    1858    1993    2088    2406 
   Size:         36      99      22     227     135      95     318

  Fragments arranged by size: 

                 960     318     227     219     135     129     129      99
                  95      36      22      12      11      10       4
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     218     883    1548    2406 
   Size:        218     665     665     858

  Fragments arranged by size: 

                 858     665     665     218
 
EcoO109I rG'GnC_Cy

Cuts at:      0     203     343     444     507     568     945    1308    1962 
   Size:        203     140     101      63      61     377     363     654

Cuts at:   1962    2234    2406 
   Size:        272     172

  Fragments arranged by size: 

                 654     377     363     272     203     172     140     101
                  63      61
 
EcoRI G'AATT_C

Cuts at:      0     639    2406 
   Size:        639    1767
 
EcoRV GAT'ATC

Cuts at:      0    1071    2406 
   Size:       1071    1335
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1749    2406 
   Size:       1749     657
 
HaeII r_GCGC'y

Cuts at:      0     378     585     852     911    1035    1446    2336    2406 
   Size:        378     207     267      59     124     411     890      70

  Fragments arranged by size: 

                 890     411     378     267     207     124      70      59
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      79     111     388     420     730     762    1215    1247 
   Size:         79      32     277      32     310      32     453      32

Cuts at:   1247    2209    2241    2406 
   Size:        962      32     165

  Fragments arranged by size: 

                 962     453     310     277     165      79      32      32
                  32      32      32
 
KasI G'GCGC_C

Cuts at:      0     848    2406 
   Size:        848    1558
 
MscI TGG'CCA

Cuts at:      0     234     306     488     904    1102    1627    2406 
   Size:        234      72     182     416     198     525     779

  Fragments arranged by size: 

                 779     525     416     234     198     182      72
 
MslI CAynn'nnrTG

Cuts at:      0     553    2192    2406 
   Size:        553    1639     214

  Fragments arranged by size: 

                1639     553     214
 
MspA1I CmG'CkG

Cuts at:      0     191     272     302     993    1091    1320    1755    1772 
   Size:        191      81      30     691      98     229     435      17

Cuts at:   1772    2406 
   Size:        634

  Fragments arranged by size: 

                 691     634     435     229     191      98      81      30
                  17
 
NarI GG'CG_CC

Cuts at:      0     849    2406 
   Size:        849    1557
 
PasI CC'CwG_GG

Cuts at:      0     198     527    2406 
   Size:        198     329    1879

  Fragments arranged by size: 

                1879     329     198
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     760    2406 
   Size:        760    1646
 
PfoI T'CCnGG_A

Cuts at:      0     829    1451    2351    2406 
   Size:        829     622     900      55

  Fragments arranged by size: 

                 900     829     622      55
 
PmlI CAC'GTG

Cuts at:      0    1998    2406 
   Size:       1998     408
 
PpuMI rG'GwC_Cy

Cuts at:      0     203     343     444     568    1308    1962    2234    2406 
   Size:        203     140     101     124     740     654     272     172

  Fragments arranged by size: 

                 740     654     272     203     172     140     124     101
            
 
PspOMI G'GGCC_C

Cuts at:      0     945    2054    2406 
   Size:        945    1109     352

  Fragments arranged by size: 

                1109     945     352
 
PstI C_TGCA'G

Cuts at:      0    1133    1307    1821    1955    1982    2406 
   Size:       1133     174     514     134      27     424

  Fragments arranged by size: 

                1133     514     424     174     134      27
 
PvuII CAG'CTG

Cuts at:      0     302    2406 
   Size:        302    2104
 
SacII CC_GC'GG

Cuts at:      0     994    2406 
   Size:        994    1412
 
SanDI GG'GwC_CC

Cuts at:      0     203     343    2406 
   Size:        203     140    2063

  Fragments arranged by size: 

                2063     203     140
 
SapI GCTCTTCn'nnn_

Cuts at:      0     806    2406 
   Size:        806    1600
 
SbfI CC_TGCA'GG

Cuts at:      0    1307    2406 
   Size:       1307    1099
 
SfcI C'TryA_G

Cuts at:      0      68    1129    1303    1817    1951    1978    2406 
   Size:         68    1061     174     514     134      27     428

  Fragments arranged by size: 

                1061     514     428     174     134      68      27
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1633    2406 
   Size:       1633     773
 
SfoI GGC'GCC

Cuts at:      0     850    2406 
   Size:        850    1556
 
SmaI CCC'GGG

Cuts at:      0     652     688     950    2406 
   Size:        652      36     262    1456

  Fragments arranged by size: 

                1456     652     262      36
 
SmlI C'TyrA_G

Cuts at:      0    1564    1648    2406 
   Size:       1564      84     758

  Fragments arranged by size: 

                1564     758      84
 
SrfI GCCC'GGGC

Cuts at:      0     652    2406 
   Size:        652    1754
 
StuI AGG'CCT

Cuts at:      0    1165    1420    1636    2305    2406 
   Size:       1165     255     216     669     101

  Fragments arranged by size: 

                1165     669     255     216     101
 
StyI C'CwwG_G

Cuts at:      0     219    1631    2204    2406 
   Size:        219    1412     573     202

  Fragments arranged by size: 

                1412     573     219     202
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2204    2283    2406 
   Size:       2204      79     123

  Fragments arranged by size: 

                2204     123      79
 
TatI w'GTAC_w

Cuts at:      0    1680    2406 
   Size:       1680     726
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1237    1269    2052    2084    2406 
   Size:       1237      32     783      32     322

  Fragments arranged by size: 

                1237     783     322      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2385    2406 
   Size:       2385      21
 
XmaI C'CCGG_G

Cuts at:      0     650     686     948    2406 
   Size:        650      36     262    1458

  Fragments arranged by size: 

                1458     650     262      36
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AhdI     AleI    AlwNI
     ApaI     ApoI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI
     BclI     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI    BsiWI Bsp1286I    BspMI    BsrBI    BsrDI    BsrGI
    BssSI   BstAPI    BstXI    BstYI     BtgI    BtgZI     BtsI     DrdI
     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI    HaeII    Hin4I     KasI     MscI     MslI   MspA1I     NarI
     PasI    PflMI     PfoI     PmlI    PpuMI   PspOMI     PstI    PvuII
    SacII    SanDI     SapI     SbfI     SfcI     SfiI     SfoI     SmaI
     SmlI     SrfI     StuI     StyI    TaqII     TatI     TstI     XcmI
     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AlfI     AloI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BcgI    BciVI
    BfrBI    BglII     BmtI     BsaI    BsaBI    BsiEI     BsmI    BsmBI
    BspEI    BspHI    BsrFI   BssHII    BstBI   BstEII  BstZ17I   Bsu36I
     ClaI    CspCI     DraI   DraIII     EagI  EcoICRI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI     PmeI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI     SalI     ScaI    SexAI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SspI     SwaI  Tth111I     XbaI
     XhoI     XmnI     ZraI
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