Restriction map for pF1KB5238
 (Linear) (Six-base) MAPSORT of: KIBB5238.seq  Check: 8294  from: 1  to: 621



KIBB5238.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AatII G_ACGT'C

Cuts at:      0     539     621 
   Size:        539      82
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     216     217     415     621 
   Size:        216       1     198     206

  Fragments arranged by size: 

                 216     206     198       1
 
ApaLI G'TGCA_C

Cuts at:      0     243     621 
   Size:        243     378
 
BanII G_rGCy'C

Cuts at:      0     100     240     621 
   Size:        100     140     381

  Fragments arranged by size: 

                 381     140     100
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     519     553     621 
   Size:        519      34      68

  Fragments arranged by size: 

                 519      68      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     317     621 
   Size:        317     304
 
Bme1580I G_kGCm'C

Cuts at:      0     247     621 
   Size:        247     374
 
BmgBI CAC'GTC

Cuts at:      0     153     519     621 
   Size:        153     366     102

  Fragments arranged by size: 

                 366     153     102
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     450     621 
   Size:        450     171
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211     621 
   Size:        211     410
 
BsaAI yAC'GTr

Cuts at:      0     402     621 
   Size:        402     219
 
BsaBI GATnn'nnATC

Cuts at:      0     595     621 
   Size:        595      26
 
BsaHI Gr'CG_yC

Cuts at:      0     158     536     621 
   Size:        158     378      85

  Fragments arranged by size: 

                 378     158      85
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     259     289     621 
   Size:        259      30     332

  Fragments arranged by size: 

                 332     259      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     111     114     621 
   Size:        111       3     507

  Fragments arranged by size: 

                 507     111       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     453     621 
   Size:        453     168
 
BsiEI CG_ry'CG

Cuts at:      0     180     621 
   Size:        180     441
 
BsiHKAI G_wGCw'C

Cuts at:      0     247     621 
   Size:        247     374
 
Bsp1286I G_dGCh'C

Cuts at:      0     100     240     247     621 
   Size:        100     140       7     374

  Fragments arranged by size: 

                 374     140     100       7
 
BsrBI CCG'CTC

Cuts at:      0     138     174     621 
   Size:        138      36     447

  Fragments arranged by size: 

                 447     138      36
 
BssSI C'ACGA_G

Cuts at:      0     139     621 
   Size:        139     482
 
BtgI C'CryG_G

Cuts at:      0      74     422     621 
   Size:         74     348     199

  Fragments arranged by size: 

                 348     199      74
 
DraIII CAC_nnn'GTG

Cuts at:      0     291     621 
   Size:        291     330
 
EaeI y'GGCC_r

Cuts at:      0     177     609     621 
   Size:        177     432      12

  Fragments arranged by size: 

                 432     177      12
 
EagI C'GGCC_G

Cuts at:      0     177     621 
   Size:        177     444
 
EarI CTCTTCn'nnn_

Cuts at:      0     120     621 
   Size:        120     501
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211     216     217     415     621 
   Size:        211       5       1     198     206

  Fragments arranged by size: 

                 211     206     198       5       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     285     621 
   Size:        285     336
 
HaeII r_GCGC'y

Cuts at:      0      10     621 
   Size:         10     611
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      97     129     621 
   Size:         97      32     492

  Fragments arranged by size: 

                 492      97      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     470     621 
   Size:        470     151
 
MscI TGG'CCA

Cuts at:      0     611     621 
   Size:        611      10
 
MspA1I CmG'CkG

Cuts at:      0      26      63      76     223     621 
   Size:         26      37      13     147     398

  Fragments arranged by size: 

                 398     147      37      26      13
 
NcoI C'CATG_G

Cuts at:      0     422     621 
   Size:        422     199
 
NspI r_CATG'y

Cuts at:      0     505     621 
   Size:        505     116
 
PfoI T'CCnGG_A

Cuts at:      0     127     621 
   Size:        127     494
 
PmlI CAC'GTG

Cuts at:      0     402     621 
   Size:        402     219
 
PstI C_TGCA'G

Cuts at:      0      91     621 
   Size:         91     530
 
PvuII CAG'CTG

Cuts at:      0     223     621 
   Size:        223     398
 
SacII CC_GC'GG

Cuts at:      0      77     621 
   Size:         77     544
 
SfcI C'TryA_G

Cuts at:      0      87     621 
   Size:         87     534
 
StyI C'CwwG_G

Cuts at:      0     422     621 
   Size:        422     199
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     502     621 
   Size:        502     119
 
XmnI GAAnn'nnTTC

Cuts at:      0     259     621 
   Size:        259     362
 
ZraI GAC'GTC

Cuts at:      0     537     621 
   Size:        537      84
 

 Enzymes that do cut:

    AatII     AcuI    ApaLI    BanII     BcgI     BglI Bme1580I    BmgBI
     BmrI     BpmI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI     BsgI
    BsiEI  BsiHKAI Bsp1286I    BsrBI    BssSI     BtgI   DraIII     EaeI
     EagI     EarI  Eco57MI    EcoNI    HaeII    Hin4I     MmeI     MscI
   MspA1I     NcoI     NspI     PfoI     PmlI     PstI    PvuII    SacII
     SfcI     StyI    TaqII     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI    BciVI     BclI    BfrBI    BglII     BlpI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaWI    BseYI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EciI  EcoICRI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MslI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
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