Restriction map for pF1KE9543
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AcyI        AflII       AgeI        AhdI        
AlwNI       AocI        Aor51HI     ApaI        ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AvaI        AviII       AvrII       BamHI       
BbeI        BciVI       BfaI        BfrI        BglI        
BlnI        BsaBI       BsaHI       BsiEI       BsiWI       
BsoBI       Bsp1407I    BspDI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      CfoI        Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     KasI        KpnI        KspI        
MaeI        MamI        MfeI        MluI        MunI        
MvnI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflMI       PinAI       PmeI        PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tsp509I     
Van91I      XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccIII        533  TCCGGA               T^CCGGA
AflIII        184  ACGTGT               A^C[GA][CT]GT
Alw44I         13  GTGCAC               G^TGCAC
AlwI          111  GATCC                GGATC(4/5)
ApaLI          13  GTGCAC               G^TGCAC
AspI          259  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI         1411  GGCACC               G^G[CT][GA]CC
BbrPI         183  CACGTG               CAC^GTG
BbsI          607  GTCTTC               GAAGAC(2/6)
BbvCI         591  GCTGAGG              CCTCAGC(-5/-2)
BcgI          485  GCACATCCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          923  TGATCA               T^GATCA
BcnI         1139  CCCGG                CC^[GC]GG
BglII         318  AGATCT               A^GATCT
BlpI         1084  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1084  GCTCAGC              GC^T[ACGT]AGC
BpuAI         607  GTCTTC               GAAGAC(2/6)
BsaAI         183  CACGTG               [CT]AC^GT[GA]
BsaWI         533  TCCGGA               [AT]^CCGG[AT]
BseAI         533  TCCGGA               T^CCGGA
BseRI          34  CTCCTC               GAGGAG(10/8)
BsmBI        1382  CGTCTC               CGTCTC(1/5)
BsmI           63  GCATTC               GAATGC(1/-1)
BspEI         533  TCCGGA               T^CCGGA
BsrDI        1025  GCAATG               GCAATG(2/0)
BssSI        1228  CTCGTG               CACGAG(-5/-1)
BstAPI        647  GCAACCACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        1084  GCTCAGC              GC^T[ACGT]AGC
DsaI          512  CCATGG               C^C[GA][CT]GG
EcoT14I       512  CCATGG               C^C[AT][AT]GG
EcoT22I        61  ATGCAT               ATGCA^T
FbaI          923  TGATCA               T^GATCA
HincII        955  GTTAAC               GT[CT]^[GA]AC
HindII        955  GTTAAC               GT[CT]^[GA]AC
HpaI          955  GTTAAC               GTT^AAC
MroI          533  TCCGGA               T^CCGGA
MseI          956  TTAA                 T^TAA
NciI         1139  CCCGG                CC^[GC]GG
NcoI          512  CCATGG               C^CATGG
NsiI           61  ATGCAT               ATGCA^T
NspI         1454  GCATGT               [GA]CATG^[CT]
PflFI         259  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         183  CACGTG               CAC^GTG
PmlI          183  CACGTG               CAC^GTG
PpuMI         823  GGGACCC              [GA]G^G[AT]CC[CT]
PshAI        1376  GACACCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI          235  GAGCTC               GAGCT^C
SapI          237  GCTCTTC              GCTCTTC(1/4)
SfcI          480  CTACAG               C^T[GA][CT]AG
StyI          512  CCATGG               C^C[AT][AT]GG
TaqI          737  TCGA                 T^CGA
Tru9I         956  TTAA                 T^TAA
Tth111I       259  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       737  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AspHI          13  GTGCAC               G[AT]GC[AT]^C
              235  GAGCTC              
BalI         1388  TGGCCA               TGG^CCA
             1481  TGGCCA              
BsaI          271  GAGACC               GGTCTC(1/5)
              769  GAGACC              
BsiHKAI        13  GTGCAC               G[AT]GC[AT]^C
              235  GAGCTC              
BstXI          95  CCATGTGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              506  CCACTGCCATGG        
BstYI         110  AGATCC               [GA]^GATC[CT]
              318  AGATCT              
EaeI         1388  TGGCCA               [CT]^GGCC[GA]
             1481  TGGCCA              
EarI          238  CTCTTC               CTCTTC(1/4)
              751  CTCTTC              
FauI          196  CCCGC                CCCGC(4/6)
             1080  GCGGG               
Ksp632I       238  CTCTTC               CTCTTC(1/4)
              751  CTCTTC              
MaeII          17  ACGT                 A^CGT
              184  ACGT                
MflI          110  AGATCC               [GA]^GATC[CT]
              318  AGATCT              
MluNI        1388  TGGCCA               TGG^CCA
             1481  TGGCCA              
MscI         1388  TGGCCA               TGG^CCA
             1481  TGGCCA              
PvuII         225  CAGCTG               CAG^CTG
             1047  CAGCTG              
XcmI          122  CCAATGAGCTGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              256  CCAGACCAAGTCTGG     
XhoII         110  AGATCC               [GA]^GATC[CT]
              318  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BanII       
BbvI        BmyI        BpmI        BsaJI       BsgI        
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EcoO109I    
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MnlI        MslI        MspA1I      
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       TfiI        TseI        Tsp45I      
TspRI       
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