Restriction map for pF1KE9543
 (Linear) (Six-base) MAPSORT of: KIEE9543.seq  Check: 6343  from: 1  to: 1488



KIEE9543.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:50  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     988    1029    1488 
   Size:        988      41     459

  Fragments arranged by size: 

                 988     459      41
 
AflIII A'CryG_T

Cuts at:      0     184    1488 
   Size:        184    1304
 
AleI CACnn'nnGTG

Cuts at:      0     704    1488 
   Size:        704     784
 
ApaLI G'TGCA_C

Cuts at:      0      13    1488 
   Size:         13    1475
 
BanI G'GyrC_C

Cuts at:      0    1411    1488 
   Size:       1411      77
 
BanII G_rGCy'C

Cuts at:      0     239    1086    1252    1488 
   Size:        239     847     166     236

  Fragments arranged by size: 

                 847     239     236     166
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     600    1488 
   Size:        600     888
 
BbvCI CC'TCA_GC

Cuts at:      0     592    1488 
   Size:        592     896
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     474     508    1488 
   Size:        474      34     980

  Fragments arranged by size: 

                 980     474      34
 
BclI T'GATC_A

Cuts at:      0     923    1488 
   Size:        923     565
 
BfrBI ATG'CAT

Cuts at:      0      63    1488 
   Size:         63    1425
 
BglII A'GATC_T

Cuts at:      0     318    1488 
   Size:        318    1170
 
BlpI GC'TnA_GC

Cuts at:      0    1085    1488 
   Size:       1085     403
 
Bme1580I G_kGCm'C

Cuts at:      0      17    1414    1488 
   Size:         17    1397      74

  Fragments arranged by size: 

                1397      74      17
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     437    1488 
   Size:        437    1051
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     137     995    1488 
   Size:        124      13     858     493

  Fragments arranged by size: 

                 858     493     124      13
 
Bpu10I CC'TnA_GC

Cuts at:      0     233     592    1429    1488 
   Size:        233     359     837      59

  Fragments arranged by size: 

                 837     359     233      59
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     265     763    1488 
   Size:        265     498     725

  Fragments arranged by size: 

                 725     498     265
 
BsaAI yAC'GTr

Cuts at:      0     185    1488 
   Size:        185    1303
 
BsaWI w'CCGG_w

Cuts at:      0     533    1488 
   Size:        533     955
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     295     325    1488 
   Size:        295      30    1163

  Fragments arranged by size: 

                1163     295      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      25    1488 
   Size:         25    1463
 
BseYI C'CCAG_C

Cuts at:      0     818     887    1239    1245    1415    1488 
   Size:        818      69     352       6     170      73

  Fragments arranged by size: 

                 818     352     170      73      69       6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     336     526     531    1015    1075    1488 
   Size:        336     190       5     484      60     413

  Fragments arranged by size: 

                 484     413     336     190      60       5
 
BsiHKAI G_wGCw'C

Cuts at:      0      17     239    1488 
   Size:         17     222    1249

  Fragments arranged by size: 

                1249     222      17
 
BsmI GAATG_Cn'

Cuts at:      0      63    1488 
   Size:         63    1425
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1388    1488 
   Size:       1388     100
 
Bsp1286I G_dGCh'C

Cuts at:      0      17     239    1086    1252    1414    1488 
   Size:         17     222     847     166     162      74

  Fragments arranged by size: 

                 847     222     166     162      74      17
 
BspEI T'CCGG_A

Cuts at:      0     533    1488 
   Size:        533     955
 
BsrDI GCAATG_nn'

Cuts at:      0    1032    1488 
   Size:       1032     456
 
BssSI C'ACGA_G

Cuts at:      0    1228    1488 
   Size:       1228     260
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     653    1488 
   Size:        653     835
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     102     513    1488 
   Size:        102     411     975

  Fragments arranged by size: 

                 975     411     102
 
BstYI r'GATC_y

Cuts at:      0     110     318    1488 
   Size:        110     208    1170

  Fragments arranged by size: 

                1170     208     110
 
BtgI C'CryG_G

Cuts at:      0     512    1488 
   Size:        512     976
 
BtsI GCAGTG_nn'

Cuts at:      0     506     543     651    1401    1488 
   Size:        506      37     108     750      87

  Fragments arranged by size: 

                 750     506     108      87      37
 
EaeI y'GGCC_r

Cuts at:      0    1388    1481    1488 
   Size:       1388      93       7

  Fragments arranged by size: 

                1388      93       7
 
EarI CTCTTCn'nnn_

Cuts at:      0     244     757    1488 
   Size:        244     513     731

  Fragments arranged by size: 

                 731     513     244
 
EcoICRI GAG'CTC

Cuts at:      0     237    1488 
   Size:        237    1251
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     137     988     995    1029    1488 
   Size:        124      13     851       7      34     459

  Fragments arranged by size: 

                 851     459     124      34      13       7
 
EcoO109I rG'GnC_Cy

Cuts at:      0     462     741     824     931    1488 
   Size:        462     279      83     107     557

  Fragments arranged by size: 

                 557     462     279     107      83
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     295     327     352     384    1488 
   Size:        295      32      25      32    1104

  Fragments arranged by size: 

                1104     295      32      32      25
 
HincII GTy'rAC

Cuts at:      0     957    1488 
   Size:        957     531
 
HpaI GTT'AAC

Cuts at:      0     957    1488 
   Size:        957     531
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     350     740    1488 
   Size:        169     181     390     748

  Fragments arranged by size: 

                 748     390     181     169
 
MscI TGG'CCA

Cuts at:      0    1390    1483    1488 
   Size:       1390      93       5

  Fragments arranged by size: 

                1390      93       5
 
MslI CAynn'nnrTG

Cuts at:      0     206     334     511     565     704     991    1488 
   Size:        206     128     177      54     139     287     497

  Fragments arranged by size: 

                 497     287     206     177     139     128      54
 
MspA1I CmG'CkG

Cuts at:      0     227    1049    1080    1488 
   Size:        227     822      31     408

  Fragments arranged by size: 

                 822     408     227      31
 
NcoI C'CATG_G

Cuts at:      0     512    1488 
   Size:        512     976
 
NsiI A_TGCA'T

Cuts at:      0      65    1488 
   Size:         65    1423
 
NspI r_CATG'y

Cuts at:      0    1458    1488 
   Size:       1458      30
 
PmlI CAC'GTG

Cuts at:      0     185    1488 
   Size:        185    1303
 
PpuMI rG'GwC_Cy

Cuts at:      0     824    1488 
   Size:        824     664
 
PshAI GACnn'nnGTC

Cuts at:      0    1380    1488 
   Size:       1380     108
 
PvuII CAG'CTG

Cuts at:      0     227    1049    1488 
   Size:        227     822     439

  Fragments arranged by size: 

                 822     439     227
 
SacI G_AGCT'C

Cuts at:      0     239    1488 
   Size:        239    1249
 
SanDI GG'GwC_CC

Cuts at:      0     824    1488 
   Size:        824     664
 
SapI GCTCTTCn'nnn_

Cuts at:      0     244    1488 
   Size:        244    1244
 
SfcI C'TryA_G

Cuts at:      0     480    1488 
   Size:        480    1008
 
StyI C'CwwG_G

Cuts at:      0     512    1488 
   Size:        512     976
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     196     289     909    1429    1488 
   Size:        196      93     620     520      59

  Fragments arranged by size: 

                 620     520     196      93      59
 
Tth111I GACn'n_nGTC

Cuts at:      0     262    1488 
   Size:        262    1226
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     129     263    1488 
   Size:        129     134    1225

  Fragments arranged by size: 

                1225     134     129
 

 Enzymes that do cut:

     AcuI   AflIII     AleI    ApaLI     BanI    BanII     BbsI    BbvCI
     BcgI     BclI    BfrBI    BglII     BlpI Bme1580I     BmrI     BpmI
   Bpu10I     BsaI    BsaAI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BsrDI    BssSI   BstAPI
    BstXI    BstYI     BtgI     BtsI     EaeI     EarI  EcoICRI  Eco57MI
 EcoO109I    Hin4I   HincII     HpaI     MmeI     MscI     MslI   MspA1I
     NcoI     NsiI     NspI     PmlI    PpuMI    PshAI    PvuII     SacI
    SanDI     SapI     SfcI     StyI    TaqII  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BciVI     BglI
    BmgBI     BmtI     BplI    BpuEI    BsaBI    BsaHI    BsiEI    BsiWI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII  HindIII     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII    SacII     SalI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI
     XbaI     XhoI     XmaI     XmnI     ZraI
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