Restriction map for pF1KB5161
##################################
# NO CUT ENZYMES (  162 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaI        
ApaLI       AscI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfrI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspMI       BsrBI       BsrDI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HgaI        Hin1I       HpaI        KasI        
KpnI        Ksp632I     KspI        MaeII       MamI        
MflI        MluI        MroI        MvnI        NaeI        
NarI        NciI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        SwaI        Tth111I     
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AcsI         1372  AAATTT               [GA]^AATT[CT]
AflIII       1359  ACATGT               A^C[GA][CT]GT
AlwNI          88  CAGGTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI         1372  AAATTT               [GA]^AATT[CT]
AseI          543  ATTAAT               AT^TAAT
AsnI          543  ATTAAT               AT^TAAT
BglI           70  GCCCAGCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI         350  GAGGAG               GAGGAG(10/8)
BsiYI          41  CCACCCTTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           41  CCACCCTTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspLU11I     1359  ACATGT               A^CATGT
BsrFI          56  ACCGGC               [GA]^CCGG[CT]
BssSI        1232  CACGAG               CACGAG(-5/-1)
BstAPI        446  GCAGAATATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI         1146  GCGC                 GCG^C
Cfr10I         56  ACCGGC               [GA]^CCGG[CT]
DraII         534  AGGTCCT              [GA]G^G[ACGT]CC[CT]
DraIII        557  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I      534  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       120  CCATGG               C^C[AT][AT]GG
FauI           75  GCGGG                CCCGC(4/6)
HapII          57  CCGG                 C^CGG
HhaI         1146  GCGC                 GCG^C
HinP1I       1146  GCGC                 G^CGC
HpaII          57  CCGG                 C^CGG
HphI          235  TCACC                GGTGA(8/7)
MfeI          254  CAATTG               C^AATTG
MspA1I         73  CAGCGG               C[AC]G^C[GT]G
MspI           57  CCGG                 C^CGG
MunI          254  CAATTG               C^AATTG
NcoI          120  CCATGG               C^CATGG
NspI         1359  ACATGT               [GA]CATG^[CT]
PpuMI         534  AGGTCCT              [GA]G^G[AT]CC[CT]
PshBI         543  ATTAAT               AT^TAAT
SspI          691  AATATT               AAT^ATT
StyI          120  CCATGG               C^C[AT][AT]GG
XcmI          215  CCAAAGGATATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AvaII         535  GGTCC                G^G[AT]CC
              881  GGACC               
BalI           38  TGGCCA               TGG^CCA
              986  TGGCCA              
BsmAI         592  GAGAC                GTCTC(1/5)
              205  GTCTC               
BsmFI         899  GGGAC                GGGAC(10/14)
             1018  GGGAC               
BsrI          878  ACTGG                ACTGG(1/-1)
             1110  ACTGG               
BssKI         488  CCAGG                ^CC[ACGT]GG
              885  CCAGG               
BstNI         488  CCAGG                CC^[AT]GG
              885  CCAGG               
DpnII         822  GATC                 ^GATC
              868  GATC                
DsaI           35  CCGTGG               C^C[GA][CT]GG
              120  CCATGG              
EcoRII        488  CCAGG                ^CC[AT]GG
              885  CCAGG               
HincII        568  GTCAAC               GT[CT]^[GA]AC
             1123  GTCAAC              
HindII        568  GTCAAC               GT[CT]^[GA]AC
             1123  GTCAAC              
MboI          822  GATC                 ^GATC
              868  GATC                
MluNI          38  TGGCCA               TGG^CCA
              986  TGGCCA              
MscI           38  TGGCCA               TGG^CCA
              986  TGGCCA              
MvaI          488  CCAGG                CC^[AT]GG
              885  CCAGG               
NdeII         822  GATC                 ^GATC
              868  GATC                
NlaIV          50  GGAACC               GG[ACGT]^[ACGT]CC
              881  GGACCC              
Sau3AI        822  GATC                 ^GATC
              868  GATC                
ScrFI         488  CCAGG                CC^[ACGT]GG
              885  CCAGG               
SfcI          871  CTACAG               C^T[GA][CT]AG
             1055  CTACAG              
TaqI          941  TCGA                 T^CGA
             1238  TCGA                
Tsp45I        234  GTCAC                ^GT[GC]AC
             1339  GTGAC               
TspRI        1253  CACTG                CA[GC]TG[ACGT][ACGT]^
             1266  CAGTG               
TthHB8I       941  TCGA                 T^CGA
             1238  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AciI        AfaI        AluI        BbvI        BfaI        
BpmI        BsaJI       BstF5I      Cac8I       Cfr13I      
DdeI        EaeI        Fnu4HI      FokI        HaeIII      
HindIII     HinfI       ItaI        MaeI        MaeIII      
MboII       MnlI        MseI        MslI        MwoI        
NlaIII      PleI        RsaI        Sau96I      SfaNI       
TfiI        Tru9I       TseI        Tsp509I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com