Restriction map for pF1KB5011
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AvrII       BamHI       BanI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglII       BlnI        BsaAI       BsaBI       
BsaHI       BsaI        BseRI       BsiEI       BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspHI       BspLU11I    BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstYI       BstZ17I     
Bsu36I      Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MflI        MluI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        SwaI        
Tth111I     XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AspHI         230  GAGCTC               G[AT]GC[AT]^C
AviII         194  TGCGCA               TGC^GCA
BanII         230  GAGCTC               G[GA]GC[CT]^C
BfaI         1067  CTAG                 C^TAG
BglI          892  GCCACTTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          230  GAGCTC               G[AGT]GC[ACT]^C
BsiHKAI       230  GAGCTC               G[AT]GC[AT]^C
BsmAI         834  GAGAC                GTCTC(1/5)
Bsp1286I      230  GAGCTC               G[AGT]GC[ACT]^C
BsrDI         888  CATTGC               GCAATG(2/0)
DraII         278  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoO109I      278  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoT14I        18  CCTTGG               C^C[AT][AT]GG
FauI          282  CCCGC                CCCGC(4/6)
FspI          194  TGCGCA               TGC^GCA
HaeII         177  GGCGCT               [GA]GCGC^[CT]
HgaI         1101  GACGC                GACGC(5/10)
MaeI         1067  CTAG                 C^TAG
MaeII         606  ACGT                 A^CGT
NlaIV         279  GGTCCC               GG[ACGT]^[ACGT]CC
NspI          480  GCATGT               [GA]CATG^[CT]
PpuMI         278  AGGTCCC              [GA]G^G[AT]CC[CT]
PstI          631  CTGCAG               CTGCA^G
SacI          230  GAGCTC               GAGCT^C
ScaI          509  AGTACT               AGT^ACT
SexAI          44  ACCTGGT              A^CC[AT]GGT
SfcI          631  CTGCAG               C^T[GA][CT]AG
SspI          751  AATATT               AAT^ATT
StyI           18  CCTTGG               C^C[AT][AT]GG
TaqI          832  TCGA                 T^CGA
Tsp509I       540  AATT                 ^AATT
TthHB8I       832  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccIII         56  TCCGGA               T^CCGGA
             1097  TCCGGA              
AfaI          510  GTAC                 GT^AC
             1197  GTAC                
AlwI           59  GGATC                GGATC(4/5)
              351  GGATC               
AvaII          93  GGACC                G^G[AT]CC
              279  GGTCC               
BalI           21  TGGCCA               TGG^CCA
              884  TGGCCA              
BlpI          105  GCTCAGC              GC^T[ACGT]AGC
              739  GCTAAGC             
BpmI          364  CTGGAG               CTGGAG(16/14)
             1241  CTGGAG              
Bpu1102I      105  GCTCAGC              GC^T[ACGT]AGC
              739  GCTAAGC             
BsaJI          18  CCTTGG               C^C[ACGT][ACGT]GG
             1214  CCCAGG              
BsaWI          56  TCCGGA               [AT]^CCGG[AT]
             1097  TCCGGA              
BseAI          56  TCCGGA               T^CCGGA
             1097  TCCGGA              
BsgI          799  CTGCAC               GTGCAG(16/14)
              766  GTGCAG              
BsmFI         503  GGGAC                GGGAC(10/14)
              280  GTCCC               
BspEI          56  TCCGGA               T^CCGGA
             1097  TCCGGA              
BspMI         710  GCAGGT               ACCTGC(4/8)
              921  GCAGGT              
BstXI         260  CCATCAGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              611  CCAACTCTATGG        
CelII         105  GCTCAGC              GC^T[ACGT]AGC
              739  GCTAAGC             
MluNI          21  TGGCCA               TGG^CCA
              884  TGGCCA              
MroI           56  TCCGGA               T^CCGGA
             1097  TCCGGA              
MscI           21  TGGCCA               TGG^CCA
              884  TGGCCA              
MseI          719  TTAA                 T^TAA
              857  TTAA                
MwoI          892  GCCACTTTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              997  GCTGTCAAAGC         
PflMI         174  CCAGGCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              357  CCAGGTGCTGG         
RsaI          510  GTAC                 GT^AC
             1197  GTAC                
SfaNI          81  GATGC                GCATC(5/9)
              979  GATGC               
SmlI          388  CTTGAG               C^T[CT][GA]AG
              695  CTCAAG              
Tru9I         719  TTAA                 T^TAA
              857  TTAA                
Van91I        174  CCAGGCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              357  CCAGGTGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AciI        AluI        AlwNI       BbsI        BbvI        
BpuAI       BsiYI       BslI        BsrI        BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       EaeI        EarI        EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeIII      MboI        MboII       MnlI        
MslI        MspA1I      MspI        MvaI        NdeII       
NlaIII      PleI        PvuII       Sau3AI      Sau96I      
ScrFI       TfiI        TseI        Tsp45I      TspRI       
XcmI        
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