Restriction map for pF1KB4989
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       Acc65I      AccIII      AciI        AclI        
AcyI        AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BbvCI       BcgI        BcnI        BglI        BlnI        
BlpI        Bpu1102I    BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
BstAPI      BstBI       BstXI       Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoRV       
EcoT14I     EcoT22I     FauI        FseI        FspI        
HaeII       HapII       Hin1I       HincII      HindII      
HindIII     HpaI        HpaII       KasI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MslI        MspI        MunI        MwoI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
StyI        SwaI        Tsp45I      Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   64 enzymes) #
###################################

AccI          435  GTATAC               GT^[AC][GT]AC
AccII         137  CGCG                 CG^CG
AfaI          741  GTAC                 GT^AC
AflII         367  CTTAAG               C^TTAAG
AseI          682  ATTAAT               AT^TAAT
AsnI          682  ATTAAT               AT^TAAT
Asp700I       564  GAAAGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         124  GAGCTC               G[AT]GC[AT]^C
AvaII         384  GGACC                G^G[AT]CC
BalI          500  TGGCCA               TGG^CCA
BanII         124  GAGCTC               G[GA]GC[CT]^C
BbsI          312  GAAGAC               GAAGAC(2/6)
BciVI         610  GGATAC               GTATCC(6/5)
BclI          594  TGATCA               T^GATCA
BfaI          391  CTAG                 C^TAG
BfrI          367  CTTAAG               C^TTAAG
BglII          53  AGATCT               A^GATCT
BmyI          124  GAGCTC               G[AGT]GC[ACT]^C
BpmI          127  CTCCAG               CTGGAG(16/14)
BpuAI         312  GAAGAC               GAAGAC(2/6)
BsaAI         238  CACGTA               [CT]AC^GT[GA]
BsaJI         543  CCAGGG               C^C[ACGT][ACGT]GG
BseRI         361  GAGGAG               GAGGAG(10/8)
BsgI          517  GTGCAG               GTGCAG(16/14)
BsiHKAI       124  GAGCTC               G[AT]GC[AT]^C
Bsp1286I      124  GAGCTC               G[AGT]GC[ACT]^C
BspHI         308  TCATGA               T^CATGA
BsrDI         605  GCAATG               GCAATG(2/0)
Bst1107I      435  GTATAC               GTA^TAC
BstEII         85  GGTTACC              G^GT[ACGT]ACC
BstPI          85  GGTTACC              G^GT[ACGT]ACC
BstUI         137  CGCG                 CG^CG
BstZ17I       435  GTATAC               GTA^TAC
CfoI          136  GCGC                 GCG^C
Cfr13I        384  GGACC                G^G[ACGT]CC
DraII         383  AGGACCT              [GA]G^G[ACGT]CC[CT]
EaeI          500  TGGCCA               [CT]^GGCC[GA]
EcoO109I      383  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO65I        85  GGTTACC              G^GT[ACGT]ACC
EcoRI         304  GAATTC               G^AATTC
FbaI          594  TGATCA               T^GATCA
HaeIII        501  GGCC                 GG^CC
HgaI           70  GACGC                GACGC(5/10)
HhaI          136  GCGC                 GCG^C
HinP1I        136  GCGC                 G^CGC
HphI          163  TCACC                GGTGA(8/7)
MaeI          391  CTAG                 C^TAG
MaeII         239  ACGT                 A^CGT
MluNI         500  TGGCCA               TGG^CCA
MscI          500  TGGCCA               TGG^CCA
MspA1I        286  CAGCTG               C[AC]G^C[GT]G
MvnI          137  CGCG                 CG^CG
NlaIV          59  GGCTCC               GG[ACGT]^[ACGT]CC
PpuMI         383  AGGACCT              [GA]G^G[AT]CC[CT]
PshBI         682  ATTAAT               AT^TAAT
PvuII         286  CAGCTG               CAG^CTG
RcaI          308  TCATGA               T^CATGA
RsaI          741  GTAC                 GT^AC
SacI          124  GAGCTC               GAGCT^C
SapI          270  GCTCTTC              GCTCTTC(1/4)
Sau96I        384  GGACC                G^G[ACGT]CC
SmlI          367  CTTAAG               C^T[CT][GA]AG
SspI          688  AATATT               AAT^ATT
XmnI          564  GAAAGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AlwNI         283  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              670  CAGGATCTG           
BspMI          14  ACCTGC               ACCTGC(4/8)
              386  ACCTGC              
BsrI          630  ACTGG                ACTGG(1/-1)
              514  CCAGT               
Cac8I         132  GCAGGC               GC[ACGT]^[ACGT]GC
              281  GCCAGC              
DdeI          359  CTGAG                C^T[ACGT]AG
              494  CTGAG               
EarI          271  CTCTTC               CTCTTC(1/4)
              253  GAAGAG              
Ksp632I       271  CTCTTC               CTCTTC(1/4)
              253  GAAGAG              
MaeIII         86  GTTAC                ^GT[ACGT]AC
              507  GTTAC               
NlaIII        309  CATG                 CATG^
              317  CATG                
PstI           16  CTGCAG               CTGCA^G
              106  CTGCAG              
SfaNI         278  GATGC                GCATC(5/9)
              634  GATGC               
SfcI           16  CTGCAG               C^T[GA][CT]AG
              106  CTGCAG              
TaqI           50  TCGA                 T^CGA
               68  TCGA                
TspRI         342  CAGTG                CA[GC]TG[ACGT][ACGT]^
              515  CAGTG               
TthHB8I        50  TCGA                 T^CGA
               68  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AcsI        AluI        AlwI        ApoI        BbvI        
BssKI       BstF5I      BstNI       BstYI       DpnII       
EcoRII      Fnu4HI      FokI        HinfI       ItaI        
MboI        MboII       MflI        MnlI        MseI        
MvaI        NdeII       Sau3AI      ScrFI       TfiI        
Tru9I       TseI        Tsp509I     XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com