Restriction map for pF1KB4957
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflIII      AgeI        AhdI        
Alw44I      AlwNI       Aor51HI     ApaI        ApaLI       
AscI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BcgI        BcnI        BglI        BlnI        BlpI        
BmyI        Bpu1102I    BsaAI       BsaHI       BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstEII      BstPI       BstUI       BstZ17I     CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO109I    
EcoO65I     EcoRV       FauI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HpaI        HpaII       
KasI        KpnI        KspI        MluI        MluNI       
MroI        MscI        MspA1I      MspI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        PacI        
PaeR7I      PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SnaBI       
Sse8387I    SspBI       StuI        SwaI        Tth111I     
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AflII         307  CTTAAG               C^TTAAG
AocI          570  CCTAAGG              CC^T[ACGT]AGG
AvaII         303  GGTCC                G^G[AT]CC
BbsI         1574  GAAGAC               GAAGAC(2/6)
BclI          801  TGATCA               T^GATCA
BfrI          307  CTTAAG               C^TTAAG
BpuAI        1574  GAAGAC               GAAGAC(2/6)
BsaBI         370  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          656  GAGACC               GGTCTC(1/5)
BseRI        1388  GAGGAG               GAGGAG(10/8)
BsmI          250  GCATTC               GAATGC(1/-1)
BspMI         845  ACCTGC               ACCTGC(4/8)
BstBI        1571  TTCGAA               TT^CGAA
BstXI        1551  CCATGAACGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        570  CCTAAGG              CC^T[ACGT]AGG
DsaI          879  CCGTGG               C^C[GA][CT]GG
EarI          145  GAAGAG               CTCTTC(1/4)
Eco81I        570  CCTAAGG              CC^T[ACGT]AGG
EcoNI         660  CCTGGATAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI          26  GAATTC               G^AATTC
EcoT14I      1072  CCAAGG               C^C[AT][AT]GG
EcoT22I       248  ATGCAT               ATGCA^T
FbaI          801  TGATCA               T^GATCA
HindIII       688  AAGCTT               A^AGCTT
HinfI         297  GAATC                G^A[ACGT]TC
HphI          114  GGTGA                GGTGA(8/7)
Ksp632I       145  GAAGAG               CTCTTC(1/4)
MamI          370  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI           71  CAATTG               C^AATTG
MslI         1552  CATGAACGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI           71  CAATTG               C^AATTG
NsiI          248  ATGCAT               ATGCA^T
NspI         1497  GCATGC               [GA]CATG^[CT]
NspV         1571  TTCGAA               TT^CGAA
PstI         1382  CTGCAG               CTGCA^G
ScaI          356  AGTACT               AGT^ACT
SfcI         1382  CTGCAG               C^T[GA][CT]AG
SfuI         1571  TTCGAA               TT^CGAA
SmlI          307  CTTAAG               C^T[CT][GA]AG
SpeI          199  ACTAGT               A^CTAGT
SphI         1497  GCATGC               GCATG^C
SspI          737  AATATT               AAT^ATT
StyI         1072  CCAAGG               C^C[AT][AT]GG
TfiI          297  GAATC                G^A[AT]TC
Tsp45I        115  GTGAC                ^GT[GC]AC
XbaI          621  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AciI          930  CCGC                 CCGC(-3/-1)
              640  GCGG                
BbvCI         490  GCTGAGG              CCTCAGC(-5/-2)
             1077  GCTGAGG             
BbvI          388  GCAGC                GCAGC(8/12)
             1501  GCAGC               
BglII         108  AGATCT               A^GATCT
             1206  AGATCT              
BpmI          409  CTCCAG               CTGGAG(16/14)
              971  CTCCAG              
BsmBI         406  CGTCTC               CGTCTC(1/5)
             1120  GAGACG              
BsmFI         995  GGGAC                GGGAC(10/14)
             1333  GGGAC               
Cac8I        1083  GCGAGC               GC[ACGT]^[ACGT]GC
             1497  GCATGC              
Cfr13I        303  GGTCC                G^G[ACGT]CC
             1427  GGCCC               
HaeIII        519  GGCC                 GG^CC
             1427  GGCC                
MwoI          388  GCAGCCAAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1384  GCAGGAGGAGC         
PflMI          39  CCAGTCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1538  CCAATAAATGG         
Sau96I        303  GGTCC                G^G[ACGT]CC
             1427  GGCCC               
TaqI         1185  TCGA                 T^CGA
             1572  TCGA                
TseI          388  GCAGC                G^C[AT]GC
             1501  GCAGC               
TthHB8I      1185  TCGA                 T^CGA
             1572  TCGA                
Van91I         39  CCAGTCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1538  CCAATAAATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
AseI        AsnI        BciVI       BfaI        BsaJI       
BsiYI       BslI        BsmAI       BsrI        BssKI       
BstF5I      BstNI       BstYI       DdeI        DpnII       
EcoRII      Fnu4HI      FokI        ItaI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MvaI        NdeII       NlaIII      
NlaIV       PshBI       RsaI        Sau3AI      ScrFI       
SfaNI       Tru9I       Tsp509I     TspRI       XhoII       
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