Restriction map for pF1KB4957
 (Linear) (Six-base) MAPSORT of: KIBB4957.seq  Check: 2720  from: 1  to: 1602



KIBB4957.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     770    1427    1602 
   Size:        770     657     175

  Fragments arranged by size: 

                 770     657     175
 
AflII C'TTAA_G

Cuts at:      0     307    1602 
   Size:        307    1295
 
ApoI r'AATT_y

Cuts at:      0      26     958    1567    1602 
   Size:         26     932     609      35

  Fragments arranged by size: 

                 932     609      35      26
 
AseI AT'TA_AT

Cuts at:      0      16     259    1190    1602 
   Size:         16     243     931     412

  Fragments arranged by size: 

                 931     412     243      16
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1581    1602 
   Size:       1581      21
 
BbvCI CC'TCA_GC

Cuts at:      0     491    1078    1602 
   Size:        491     587     524

  Fragments arranged by size: 

                 587     524     491
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     408     423    1545    1602 
   Size:        408      15    1122      57

  Fragments arranged by size: 

                1122     408      57      15
 
BclI T'GATC_A

Cuts at:      0     801    1602 
   Size:        801     801
 
BfrBI ATG'CAT

Cuts at:      0     250    1602 
   Size:        250    1352
 
BglII A'GATC_T

Cuts at:      0     108    1206    1602 
   Size:        108    1098     396

  Fragments arranged by size: 

                1098     396     108
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      33    1602 
   Size:         33    1569
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     394     956    1602 
   Size:        394     562     646

  Fragments arranged by size: 

                 646     562     394
 
Bpu10I CC'TnA_GC

Cuts at:      0     491    1078    1602 
   Size:        491     587     524

  Fragments arranged by size: 

                 587     524     491
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     650    1602 
   Size:        650     952
 
BsaBI GATnn'nnATC

Cuts at:      0     374    1602 
   Size:        374    1228
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1382    1406    1412    1436    1602 
   Size:       1382      24       6      24     166

  Fragments arranged by size: 

                1382     166      24      24       6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1403    1602 
   Size:       1403     199
 
BseYI C'CCAG_C

Cuts at:      0    1329    1602 
   Size:       1329     273
 
BsmI GAATG_Cn'

Cuts at:      0     250    1602 
   Size:        250    1352
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     412    1114    1602 
   Size:        412     702     488

  Fragments arranged by size: 

                 702     488     412
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     854    1602 
   Size:        854     748
 
BstBI TT'CG_AA

Cuts at:      0    1572    1602 
   Size:       1572      30
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1558    1602 
   Size:       1558      44
 
BstYI r'GATC_y

Cuts at:      0     108     216     702    1206    1602 
   Size:        108     108     486     504     396

  Fragments arranged by size: 

                 504     486     396     108     108
 
Bsu36I CC'TnA_GG

Cuts at:      0     571    1602 
   Size:        571    1031
 
BtgI C'CryG_G

Cuts at:      0     879    1602 
   Size:        879     723
 
EarI CTCTTCn'nnn_

Cuts at:      0     140    1602 
   Size:        140    1462
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     394     770     956    1427    1602 
   Size:        394     376     186     471     175

  Fragments arranged by size: 

                 471     394     376     186     175
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     664    1602 
   Size:        664     938
 
EcoRI G'AATT_C

Cuts at:      0      26    1602 
   Size:         26    1576
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     201     233     427     459     598     630     781     813 
   Size:        201      32     194      32     139      32     151      32

Cuts at:    813    1602 
   Size:        789

  Fragments arranged by size: 

                 789     201     194     151     139      32      32      32
                  32
 
HindIII A'AGCT_T

Cuts at:      0     688    1602 
   Size:        688     914
 
MfeI C'AATT_G

Cuts at:      0      71    1602 
   Size:         71    1531
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     272    1245    1392    1602 
   Size:        272     973     147     210

  Fragments arranged by size: 

                 973     272     210     147
 
MslI CAynn'nnrTG

Cuts at:      0    1556    1602 
   Size:       1556      46
 
NsiI A_TGCA'T

Cuts at:      0     252    1602 
   Size:        252    1350
 
NspI r_CATG'y

Cuts at:      0    1501    1602 
   Size:       1501     101
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      45    1544    1602 
   Size:         45    1499      58

  Fragments arranged by size: 

                1499      58      45
 
PfoI T'CCnGG_A

Cuts at:      0     410     459    1602 
   Size:        410      49    1143

  Fragments arranged by size: 

                1143     410      49
 
PstI C_TGCA'G

Cuts at:      0    1386    1602 
   Size:       1386     216
 
ScaI AGT'ACT

Cuts at:      0     358    1602 
   Size:        358    1244
 
SfcI C'TryA_G

Cuts at:      0    1382    1602 
   Size:       1382     220
 
SmlI C'TyrA_G

Cuts at:      0     307    1602 
   Size:        307    1295
 
SpeI A'CTAG_T

Cuts at:      0     199    1602 
   Size:        199    1403
 
SphI G_CATG'C

Cuts at:      0    1501    1602 
   Size:       1501     101
 
SspI AAT'ATT

Cuts at:      0     739    1602 
   Size:        739     863
 
StyI C'CwwG_G

Cuts at:      0    1072    1602 
   Size:       1072     530
 
TatI w'GTAC_w

Cuts at:      0     202     356    1489    1602 
   Size:        202     154    1133     113

  Fragments arranged by size: 

                1133     202     154     113
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1386    1418    1602 
   Size:       1386      32     184

  Fragments arranged by size: 

                1386     184      32
 
XbaI T'CTAG_A

Cuts at:      0     621    1602 
   Size:        621     981
 

 Enzymes that do cut:

     AcuI    AflII     ApoI     AseI     BbsI    BbvCI    BciVI     BclI
    BfrBI    BglII     BmrI     BpmI   Bpu10I     BsaI    BsaBI    BsaXI
    BseRI    BseYI     BsmI    BsmBI    BspMI    BstBI    BstXI    BstYI
   Bsu36I     BtgI     EarI  Eco57MI    EcoNI    EcoRI    Hin4I  HindIII
     MfeI     MmeI     MslI     NsiI     NspI    PflMI     PfoI     PstI
     ScaI     SfcI     SmlI     SpeI     SphI     SspI     StyI     TatI
     TstI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BcgI     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI    BpuEI
    BsaAI    BsaHI    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI Bsp1286I
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI   BstEII  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI EcoO109I    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI
     KpnI     MluI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SrfI     StuI     SwaI    TaqII  Tth111I     XcmI     XhoI     XmaI
     XmnI     ZraI
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