Restriction map for pF1KB4900
##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsiEI       BsiWI       
BsmBI       BsoBI       BspDI       BspMI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraIII      EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRV       
EcoT22I     FseI        FspI        Hin1I       KasI        
KpnI        KspI        MamI        MfeI        MluI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        SwaI        Tth111I     
Van91I      XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccIII       1433  TCCGGA               T^CCGGA
AcsI         1325  GAATTC               [GA]^AATT[CT]
AflIII        527  ACATGT               A^C[GA][CT]GT
AocI         1330  CCTCAGG              CC^T[ACGT]AGG
ApoI         1325  GAATTC               [GA]^AATT[CT]
Asp700I       525  GAACATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI           83  TGGCCA               TGG^CCA
BanII          22  GAGCCC               G[GA]GC[CT]^C
BclI          518  TGATCA               T^GATCA
BcnI          225  CCGGG                CC^[GC]GG
BpmI          303  CTCCAG               CTGGAG(16/14)
BsaWI        1433  TCCGGA               [AT]^CCGG[AT]
BseAI        1433  TCCGGA               T^CCGGA
BsgI           97  GTGCAG               GTGCAG(16/14)
Bsp1407I      947  TGTACA               T^GTACA
BspEI        1433  TCCGGA               T^CCGGA
BspHI         833  TCATGA               T^CATGA
BspLU11I      527  ACATGT               A^CATGT
BsrBI        1073  CCGCTC               CCGCTC(-3/-3)
BsrGI         947  TGTACA               T^GTACA
BstYI         706  GGATCT               [GA]^GATC[CT]
Bsu36I       1330  CCTCAGG              CC^T[ACGT]AGG
DrdI         1147  GACAAGCGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI           83  TGGCCA               [CT]^GGCC[GA]
Eco81I       1330  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1209  CCTCTATGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1325  GAATTC               G^AATTC
FauI         1336  GCGGG                CCCGC(4/6)
FbaI          518  TGATCA               T^GATCA
HaeII         327  GGCGCT               [GA]GCGC^[CT]
HgaI         1154  GCGTC                GACGC(5/10)
HincII       1246  GTTAAC               GT[CT]^[GA]AC
HindII       1246  GTTAAC               GT[CT]^[GA]AC
HindIII       591  AAGCTT               A^AGCTT
HpaI         1246  GTTAAC               GTT^AAC
MaeII        1448  ACGT                 A^CGT
MflI          706  GGATCT               [GA]^GATC[CT]
MluNI          83  TGGCCA               TGG^CCA
MroI         1433  TCCGGA               T^CCGGA
MscI           83  TGGCCA               TGG^CCA
NciI          225  CCGGG                CC^[GC]GG
NcoI          659  CCATGG               C^CATGG
PpuMI        1081  GGGACCT              [GA]G^G[AT]CC[CT]
PshAI        1365  GACATCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          497  CTGCAG               CTGCA^G
RcaI          833  TCATGA               T^CATGA
SapI           19  GAAGAGC              GCTCTTC(1/4)
ScaI          236  AGTACT               AGT^ACT
SmlI         1241  CTCAAG               C^T[CT][GA]AG
SspBI         947  TGTACA               T^GTACA
TaqI         1607  TCGA                 T^CGA
TthHB8I      1607  TCGA                 T^CGA
XhoII         706  GGATCT               [GA]^GATC[CT]
XmnI          525  GAACATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII        1153  CGCG                 CG^CG
             1407  CGCG                
AlwI          706  GGATC                GGATC(4/5)
             1425  GGATC               
AspHI        1168  GTGCTC               G[AT]GC[AT]^C
             1483  GAGCAC              
BbsI          913  GAAGAC               GAAGAC(2/6)
              742  GTCTTC              
BfaI          331  CTAG                 C^TAG
              736  CTAG                
BpuAI         913  GAAGAC               GAAGAC(2/6)
              742  GTCTTC              
BseRI        1003  CTCCTC               GAGGAG(10/8)
              685  GAGGAG              
BsiHKAI      1168  GTGCTC               G[AT]GC[AT]^C
             1483  GAGCAC              
BsmI          141  GAATGC               GAATGC(1/-1)
              359  GAATGC              
BstUI        1153  CGCG                 CG^CG
             1407  CGCG                
BstXI        1187  CCATTAACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1445  CCAACGTACTGG        
CfoI          328  GCGC                 GCG^C
             1152  GCGC                
DraII         794  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1081  GGGACCT             
DsaI          659  CCATGG               C^C[GA][CT]GG
              869  CCGTGG              
EcoO109I      794  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1081  GGGACCT             
HapII         225  CCGG                 C^CGG
             1434  CCGG                
HhaI          328  GCGC                 GCG^C
             1152  GCGC                
HinP1I        328  GCGC                 G^CGC
             1152  GCGC                
HpaII         225  CCGG                 C^CGG
             1434  CCGG                
MaeI          331  CTAG                 C^TAG
              736  CTAG                
MspI          225  CCGG                 C^CGG
             1434  CCGG                
MvnI         1153  CGCG                 CG^CG
             1407  CGCG                
NspI          461  ACATGC               [GA]CATG^[CT]
              527  ACATGT              
SfcI          368  CTGTAG               C^T[GA][CT]AG
              497  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       AvaII       
BbvI        BmyI        BsaJI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrDI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       DraI        EarI        EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        Ksp632I     MaeIII      MboI        
MboII       MnlI        MseI        MslI        MspA1I      
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PleI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XcmI        
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