Restriction map for pF1KB4900
 (Linear) (Six-base) MAPSORT of: KIBB4900.seq  Check: 9033  from: 1  to: 1626



KIBB4900.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1525    1626 
   Size:       1525     101
 
AflIII A'CryG_T

Cuts at:      0     527    1626 
   Size:        527    1099
 
AlwNI CAG_nnn'CTG

Cuts at:      0     282     709     798    1626 
   Size:        282     427      89     828

  Fragments arranged by size: 

                 828     427     282      89
 
ApoI r'AATT_y

Cuts at:      0    1325    1626 
   Size:       1325     301
 
BanII G_rGCy'C

Cuts at:      0      26    1626 
   Size:         26    1600
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     735     920    1626 
   Size:        735     185     706

  Fragments arranged by size: 

                 735     706     185
 
BclI T'GATC_A

Cuts at:      0     518    1626 
   Size:        518    1108
 
Bme1580I G_kGCm'C

Cuts at:      0     451     605    1626 
   Size:        451     154    1021

  Fragments arranged by size: 

                1021     451     154
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     691    1462    1626 
   Size:        691     771     164

  Fragments arranged by size: 

                 771     691     164
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     288    1626 
   Size:        288    1338
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1226    1626 
   Size:       1226     400
 
BsaWI w'CCGG_w

Cuts at:      0    1433    1626 
   Size:       1433     193
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1474    1504    1626 
   Size:       1474      30     122

  Fragments arranged by size: 

                1474     122      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     700     994    1626 
   Size:        700     294     632

  Fragments arranged by size: 

                 700     632     294
 
BseYI C'CCAG_C

Cuts at:      0     892    1471    1626 
   Size:        892     579     155

  Fragments arranged by size: 

                 892     579     155
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118    1626 
   Size:        118    1508
 
BsiHKAI G_wGCw'C

Cuts at:      0    1172    1487    1626 
   Size:       1172     315     139

  Fragments arranged by size: 

                1172     315     139
 
BsmI GAATG_Cn'

Cuts at:      0     147     365    1626 
   Size:        147     218    1261

  Fragments arranged by size: 

                1261     218     147
 
Bsp1286I G_dGCh'C

Cuts at:      0      26     451     605    1172    1487    1626 
   Size:         26     425     154     567     315     139

  Fragments arranged by size: 

                 567     425     315     154     139      26
 
BspEI T'CCGG_A

Cuts at:      0    1433    1626 
   Size:       1433     193
 
BspHI T'CATG_A

Cuts at:      0     833    1626 
   Size:        833     793
 
BsrBI CCG'CTC

Cuts at:      0    1075    1626 
   Size:       1075     551
 
BsrDI GCAATG_nn'

Cuts at:      0      18     395    1235    1626 
   Size:         18     377     840     391

  Fragments arranged by size: 

                 840     391     377      18
 
BsrGI T'GTAC_A

Cuts at:      0     947    1626 
   Size:        947     679
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1194    1452    1626 
   Size:       1194     258     174

  Fragments arranged by size: 

                1194     258     174
 
BstYI r'GATC_y

Cuts at:      0     706    1626 
   Size:        706     920
 
Bsu36I CC'TnA_GG

Cuts at:      0    1331    1626 
   Size:       1331     295
 
BtgI C'CryG_G

Cuts at:      0     659     869    1626 
   Size:        659     210     757

  Fragments arranged by size: 

                 757     659     210
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1389    1626 
   Size:       1389     237
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     378     413    1626 
   Size:        378      35    1213

  Fragments arranged by size: 

                1213     378      35
 
DraI TTT'AAA

Cuts at:      0     561     570     597    1626 
   Size:        561       9      27    1029

  Fragments arranged by size: 

                1029     561      27       9
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1153    1626 
   Size:       1153     473
 
EaeI y'GGCC_r

Cuts at:      0      83    1626 
   Size:         83    1543
 
EarI CTCTTCn'nnn_

Cuts at:      0      14     580     886    1092    1124    1538    1626 
   Size:         14     566     306     206      32     414      88

  Fragments arranged by size: 

                 566     414     306     206      88      32      14
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     288    1525    1626 
   Size:        288    1237     101

  Fragments arranged by size: 

                1237     288     101
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1213    1626 
   Size:       1213     413
 
EcoO109I rG'GnC_Cy

Cuts at:      0     795    1082    1626 
   Size:        795     287     544

  Fragments arranged by size: 

                 795     544     287
 
EcoRI G'AATT_C

Cuts at:      0    1325    1626 
   Size:       1325     301
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     609    1626 
   Size:        609    1017
 
HaeII r_GCGC'y

Cuts at:      0     331    1626 
   Size:        331    1295
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     185     217     356     388    1154    1186    1474    1506 
   Size:        185      32     139      32     766      32     288      32

Cuts at:   1506    1626 
   Size:        120

  Fragments arranged by size: 

                 766     288     185     139     120      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1248    1626 
   Size:       1248     378
 
HindIII A'AGCT_T

Cuts at:      0     591    1626 
   Size:        591    1035
 
HpaI GTT'AAC

Cuts at:      0    1248    1626 
   Size:       1248     378
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     228    1626 
   Size:        228    1398
 
MscI TGG'CCA

Cuts at:      0      85    1626 
   Size:         85    1541
 
MslI CAynn'nnrTG

Cuts at:      0     319     400     922    1192    1408    1626 
   Size:        319      81     522     270     216     218

  Fragments arranged by size: 

                 522     319     270     218     216      81
 
MspA1I CmG'CkG

Cuts at:      0     169     496    1303    1321    1626 
   Size:        169     327     807      18     305

  Fragments arranged by size: 

                 807     327     305     169      18
 
NcoI C'CATG_G

Cuts at:      0     659    1626 
   Size:        659     967
 
NspI r_CATG'y

Cuts at:      0     465     531    1626 
   Size:        465      66    1095

  Fragments arranged by size: 

                1095     465      66
 
PasI CC'CwG_GG

Cuts at:      0     729    1464    1626 
   Size:        729     735     162

  Fragments arranged by size: 

                 735     729     162
 
PciI A'CATG_T

Cuts at:      0     527    1626 
   Size:        527    1099
 
PfoI T'CCnGG_A

Cuts at:      0     509     702    1421    1626 
   Size:        509     193     719     205

  Fragments arranged by size: 

                 719     509     205     193
 
PpuMI rG'GwC_Cy

Cuts at:      0    1082    1626 
   Size:       1082     544
 
PshAI GACnn'nnGTC

Cuts at:      0    1369    1626 
   Size:       1369     257
 
PstI C_TGCA'G

Cuts at:      0     501    1626 
   Size:        501    1125
 
PvuII CAG'CTG

Cuts at:      0     496    1303    1321    1626 
   Size:        496     807      18     305

  Fragments arranged by size: 

                 807     496     305      18
 
SapI GCTCTTCn'nnn_

Cuts at:      0      14    1626 
   Size:         14    1612
 
ScaI AGT'ACT

Cuts at:      0     238    1626 
   Size:        238    1388
 
SfcI C'TryA_G

Cuts at:      0     368     497    1626 
   Size:        368     129    1129

  Fragments arranged by size: 

                1129     368     129
 
SmlI C'TyrA_G

Cuts at:      0    1241    1626 
   Size:       1241     385
 
StyI C'CwwG_G

Cuts at:      0     189     659    1033    1626 
   Size:        189     470     374     593

  Fragments arranged by size: 

                 593     470     374     189
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     619     910    1626 
   Size:        619     291     716

  Fragments arranged by size: 

                 716     619     291
 
TatI w'GTAC_w

Cuts at:      0     236     947    1626 
   Size:        236     711     679

  Fragments arranged by size: 

                 711     679     236
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     399     837    1191    1449    1626 
   Size:        399     438     354     258     177

  Fragments arranged by size: 

                 438     399     354     258     177
 
XmnI GAAnn'nnTTC

Cuts at:      0     529    1626 
   Size:        529    1097
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApoI    BanII     BbsI     BclI Bme1580I
     BmrI     BpmI    BpuEI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrGI
    BstXI    BstYI   Bsu36I     BtgI    BtgZI    CspCI     DraI     DrdI
     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII
    Hin4I   HincII  HindIII     HpaI     MmeI     MscI     MslI   MspA1I
     NcoI     NspI     PasI     PciI     PfoI    PpuMI    PshAI     PstI
    PvuII     SapI     ScaI     SfcI     SmlI     StyI    TaqII     TatI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI
     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsiEI    BsiWI
    BsmBI    BspMI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I     BtsI     ClaI   DraIII     EagI     EciI  EcoICRI    EcoRV
     FseI     FspI    FspAI     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
    PflMI     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XhoI
     XmaI     ZraI
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