Restriction map for pF1KB4508
##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        Asp700I     Asp718I     AspEI       
AvaI        AviII       AvrII       BamHI       BanI        
BbeI        BbrPI       BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BlnI        BsaAI       
BsaBI       BsaHI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrBI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DraIII      EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRV       EcoT22I     
FbaI        FseI        FspI        HgaI        Hin1I       
HindIII     HpaI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MamI        MfeI        
MluI        MroI        MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PstI        PvuI        PvuII       RsrII       SacI        
SacII       SalI        SapI        SbfI        SfiI        
SfoI        SfuI        SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        TthHB8I     XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

AgeI          115  ACCGGT               A^CCGGT
AlwI          787  GGATC                GGATC(4/5)
AseI          345  ATTAAT               AT^TAAT
AsnI          345  ATTAAT               AT^TAAT
AspHI         163  GTGCTC               G[AT]GC[AT]^C
AspI          812  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         513  GGACC                G^G[AT]CC
BalI         1031  TGGCCA               TGG^CCA
BbsI          523  GTCTTC               GAAGAC(2/6)
BbvCI         991  GCTGAGG              CCTCAGC(-5/-2)
BcnI          189  CCGGG                CC^[GC]GG
BglII         695  AGATCT               A^GATCT
BpuAI         523  GTCTTC               GAAGAC(2/6)
BsaI          303  GGTCTC               GGTCTC(1/5)
BsaWI         115  ACCGGT               [AT]^CCGG[AT]
BseRI         753  CTCCTC               GAGGAG(10/8)
BsiHKAI       163  GTGCTC               G[AT]GC[AT]^C
BspHI        1052  TCATGA               T^CATGA
BspMI        1130  ACCTGC               ACCTGC(4/8)
BsrFI         115  ACCGGT               [GA]^CCGG[CT]
BstF5I        744  CATCC                GGATG(2/0)
BstYI         695  AGATCT               [GA]^GATC[CT]
CfoI          907  GCGC                 GCG^C
Cfr10I        115  ACCGGT               [GA]^CCGG[CT]
DraII         512  GGGACCT              [GA]G^G[ACGT]CC[CT]
DrdI          514  GACCTCCCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         1031  TGGCCA               [CT]^GGCC[GA]
EcoO109I      512  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI         492  GAATTC               G^AATTC
EcoT14I       715  CCATGG               C^C[AT][AT]GG
FauI          808  GCGGG                CCCGC(4/6)
FokI          744  CATCC                GGATG(9/13)
HaeII         906  GGCGCT               [GA]GCGC^[CT]
HhaI          907  GCGC                 GCG^C
HinP1I        907  GCGC                 G^CGC
HincII        214  GTCAAC               GT[CT]^[GA]AC
HindII        214  GTCAAC               GT[CT]^[GA]AC
MflI          695  AGATCT               [GA]^GATC[CT]
MluNI        1031  TGGCCA               TGG^CCA
MscI         1031  TGGCCA               TGG^CCA
MspA1I        806  CAGCGG               C[AC]G^C[GT]G
NciI          189  CCGGG                CC^[GC]GG
NcoI          715  CCATGG               C^CATGG
NspI          774  GCATGC               [GA]CATG^[CT]
PflFI         812  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         924  CCACCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         115  ACCGGT               A^CCGGT
PpuMI         512  GGGACCT              [GA]G^G[AT]CC[CT]
PshBI         345  ATTAAT               AT^TAAT
RcaI         1052  TCATGA               T^CATGA
ScaI          390  AGTACT               AGT^ACT
SexAI          59  ACCAGGT              A^CC[AT]GGT
SfcI          620  CTACAG               C^T[GA][CT]AG
SgrAI         114  CACCGGTG             C[GA]^CCGG[CT]G
SmlI          480  CTCAAG               C^T[CT][GA]AG
SphI          774  GCATGC               GCATG^C
StyI          715  CCATGG               C^C[AT][AT]GG
Tth111I       812  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        924  CCACCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         695  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AciI         1119  CCGC                 CCGC(-3/-1)
              808  GCGG                
AcsI          492  GAATTC               [GA]^AATT[CT]
             1065  AAATTC              
ApoI          492  GAATTC               [GA]^AATT[CT]
             1065  AAATTC              
BanII         275  GGGCTC               G[GA]GC[CT]^C
              358  GGGCTC              
BbvI          805  GCAGC                GCAGC(8/12)
              778  GCTGC               
BlpI          327  GCTCAGC              GC^T[ACGT]AGC
              501  GCTCAGC             
BpmI          166  CTCCAG               CTGGAG(16/14)
              658  CTGGAG              
Bpu1102I      327  GCTCAGC              GC^T[ACGT]AGC
              501  GCTCAGC             
BsmAI         419  GAGAC                GTCTC(1/5)
              304  GTCTC               
BsmFI         512  GGGAC                GGGAC(10/14)
              810  GGGAC               
BstXI         308  CCACTCTGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              377  CCACCCTGGTGG        
CelII         327  GCTCAGC              GC^T[ACGT]AGC
              501  GCTCAGC             
DpnII         696  GATC                 ^GATC
              788  GATC                
DsaI          158  CCGTGG               C^C[GA][CT]GG
              715  CCATGG              
Fnu4HI        778  GCTGC                GC^[ACGT]GC
              805  GCAGC               
HapII         116  CCGG                 C^CGG
              189  CCGG                
HpaII         116  CCGG                 C^CGG
              189  CCGG                
ItaI          778  GCTGC                GC^[ACGT]GC
              805  GCAGC               
MboI          696  GATC                 ^GATC
              788  GATC                
MseI          146  TTAA                 T^TAA
              346  TTAA                
MspI          116  CCGG                 C^CGG
              189  CCGG                
MwoI          877  GCCTGCATTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              909  GCTCACCTGGC         
NdeII         696  GATC                 ^GATC
              788  GATC                
NlaIV         512  GGGACC               GG[ACGT]^[ACGT]CC
              739  GGAGCC              
Sau3AI        696  GATC                 ^GATC
              788  GATC                
Tru9I         146  TTAA                 T^TAA
              346  TTAA                
TseI          778  GCTGC                G^C[AT]GC
              805  GCAGC               
Tsp45I        112  GTCAC                ^GT[GC]AC
              119  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AfaI        AluI        BmyI        BsaJI       BsiYI       
BslI        Bsp1286I    BsrDI       BsrI        BssKI       
BstNI       Cac8I       Cfr13I      DdeI        EcoRII      
HaeIII      HinfI       HphI        MaeIII      MboII       
MnlI        MslI        MvaI        NlaIII      PleI        
RsaI        Sau96I      ScrFI       SfaNI       TfiI        
Tsp509I     TspRI       XcmI        
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