Restriction map for pF1KB4255
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AfaI        AflII       AflIII      AgeI        AhdI        
AlwNI       AocI        Aor51HI     AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BbrPI       BbvCI       
BcgI        BclI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaI        BsgI        BsiEI       BsiWI       BsmAI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspHI       BspLU11I    BspMI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DdeI        DraI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FokI        FseI        FspI        HgaI        HincII      
HindII      HindIII     HpaI        HphI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MaeIII      
MamI        MfeI        MluI        MseI        MslI        
MunI        MvnI        NaeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        TfiI        Tru9I       
Tsp45I      Tth111I     TthHB8I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccIII        271  TCCGGA               T^CCGGA
AciI          524  CCGC                 CCGC(-3/-1)
AcsI           10  GAATTT               [GA]^AATT[CT]
AcyI          459  GGCGCC               G[GA]^CG[CT]C
Alw44I        392  GTGCAC               G^TGCAC
ApaI          324  GGGCCC               GGGCC^C
ApaLI         392  GTGCAC               G^TGCAC
ApoI           10  GAATTT               [GA]^AATT[CT]
BamHI         436  GGATCC               G^GATCC
BanII         324  GGGCCC               G[GA]GC[CT]^C
BbeI          459  GGCGCC               GGCGC^C
BbsI          444  GTCTTC               GAAGAC(2/6)
BcnI          322  CCGGG                CC^[GC]GG
BglI          544  GCCCCTGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         444  GTCTTC               GAAGAC(2/6)
BsaHI         459  GGCGCC               G[GA]^CG[CT]C
BseAI         271  TCCGGA               T^CCGGA
BspEI         271  TCCGGA               T^CCGGA
BsrBI         524  CCGCTC               CCGCTC(-3/-3)
BsrI          662  CCAGT                ACTGG(1/-1)
Cac8I          98  GCTGGC               GC[ACGT]^[ACGT]GC
CfoI          460  GCGC                 GCG^C
DrdI          275  GACCATCATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI          88  CCTCCTCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI          523  CCCGC                CCCGC(4/6)
HaeII         459  GGCGCC               [GA]GCGC^[CT]
HhaI          460  GCGC                 GCG^C
Hin1I         459  GGCGCC               G[GA]^CG[CT]C
HinP1I        460  GCGC                 G^CGC
KasI          459  GGCGCC               G^GCGCC
MroI          271  TCCGGA               T^CCGGA
NarI          459  GGCGCC               GG^CGCC
NciI          322  CCGGG                CC^[GC]GG
NdeI          386  CATATG               CA^TATG
PstI          410  CTGCAG               CTGCA^G
SexAI         291  ACCTGGT              A^CC[AT]GGT
SfaNI          25  GATGC                GCATC(5/9)
SfoI          459  GGCGCC               GGC^GCC
SmlI           92  CTCAAG               C^T[CT][GA]AG
Tsp509I        11  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

BalI           81  TGGCCA               TGG^CCA
              100  TGGCCA              
BanI          459  GGCGCC               G^G[CT][GA]CC
              574  GGCACC              
BpmI          242  CTCCAG               CTGGAG(16/14)
              509  CTCCAG              
BsaWI         232  ACCGGA               [AT]^CCGG[AT]
              271  TCCGGA              
BsmFI         597  GGGAC                GGGAC(10/14)
              284  GTCCC               
BstXI         540  CCAAGCCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              586  CCAGGAGCCTGG        
BstYI         402  AGATCC               [GA]^GATC[CT]
              436  GGATCC              
DraIII        161  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
              290  CACCTGGTG           
DsaI          430  CCATGG               C^C[GA][CT]GG
              568  CCATGG              
EaeI           81  TGGCCA               [CT]^GGCC[GA]
              100  TGGCCA              
HinfI         155  GACTC                G^A[ACGT]TC
              647  GACTC               
MboII         139  TCTTC                GAAGA(8/7)
              445  TCTTC               
MflI          402  AGATCC               [GA]^GATC[CT]
              436  GGATCC              
MluNI          81  TGGCCA               TGG^CCA
              100  TGGCCA              
MscI           81  TGGCCA               TGG^CCA
              100  TGGCCA              
MspA1I        407  CAGCTG               C[AC]G^C[GT]G
              413  CAGCTG              
MwoI           98  GCTGGCCAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              544  GCCCCTGGGGC         
NcoI          430  CCATGG               C^CATGG
              568  CCATGG              
PleI          155  GACTC                GAGTC(4/5)
              647  GACTC               
PpuMI         246  AGGACCC              [GA]G^G[AT]CC[CT]
              687  AGGACCT             
PvuII         407  CAGCTG               CAG^CTG
              413  CAGCTG              
SfcI          410  CTGCAG               C^T[GA][CT]AG
              623  CTACAG              
TspRI         143  CAGTG                CA[GC]TG[ACGT][ACGT]^
              180  CACTG               
XcmI          559  CCACCTGTTCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              583  CCACCAGGAGCCTGG     
XhoII         402  AGATCC               [GA]^GATC[CT]
              436  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AluI        AlwI        AspHI       AvaII       BbvI        
BciVI       BmyI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        Bsp1286I    BssKI       BstNI       
Cfr13I      DpnII       DraII       EcoO109I    EcoRII      
EcoT14I     Fnu4HI      HaeIII      HapII       HpaII       
ItaI        MboI        MnlI        MspI        MvaI        
NdeII       NlaIII      NlaIV       PflMI       Sau3AI      
Sau96I      ScrFI       StyI        TseI        Van91I      
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