Restriction map for pF1KB4255
 (Linear) (Six-base) MAPSORT of: KIBB4255.seq  Check: 1726  from: 1  to: 735



KIBB4255.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     125     668     735 
   Size:        125     543      67

  Fragments arranged by size: 

                 543     125      67
 
ApaI G_GGCC'C

Cuts at:      0     328     735 
   Size:        328     407
 
ApaLI G'TGCA_C

Cuts at:      0     392     735 
   Size:        392     343
 
ApoI r'AATT_y

Cuts at:      0      10     735 
   Size:         10     725
 
BamHI G'GATC_C

Cuts at:      0     436     735 
   Size:        436     299
 
BanI G'GyrC_C

Cuts at:      0     459     574     735 
   Size:        459     115     161

  Fragments arranged by size: 

                 459     161     115
 
BanII G_rGCy'C

Cuts at:      0     328     735 
   Size:        328     407
 
BbeI G_GCGC'C

Cuts at:      0     463     735 
   Size:        463     272
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     437     735 
   Size:        437     298
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     224     491     644     735 
   Size:        224     267     153      91

  Fragments arranged by size: 

                 267     224     153      91
 
BglI GCCn_nnn'nGGC

Cuts at:      0     550     735 
   Size:        550     185
 
Bme1580I G_kGCm'C

Cuts at:      0     328     396     577     735 
   Size:        328      68     181     158

  Fragments arranged by size: 

                 328     181     158      68
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     656     735 
   Size:        656      79
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     227     494     735 
   Size:        227     267     241

  Fragments arranged by size: 

                 267     241     227
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      77     735 
   Size:         77     658
 
BsaHI Gr'CG_yC

Cuts at:      0     460     735 
   Size:        460     275
 
BsaWI w'CCGG_w

Cuts at:      0     232     271     735 
   Size:        232      39     464

  Fragments arranged by size: 

                 464     232      39
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      80     518     521     524     735 
   Size:         80     438       3       3     211

  Fragments arranged by size: 

                 438     211      80       3       3
 
BseYI C'CCAG_C

Cuts at:      0     252     735 
   Size:        252     483
 
BsiHKAI G_wGCw'C

Cuts at:      0     135     396     702     735 
   Size:        135     261     306      33

  Fragments arranged by size: 

                 306     261     135      33
 
Bsp1286I G_dGCh'C

Cuts at:      0     135     328     396     577     702     735 
   Size:        135     193      68     181     125      33

  Fragments arranged by size: 

                 193     181     135     125      68      33
 
BspEI T'CCGG_A

Cuts at:      0     271     735 
   Size:        271     464
 
BsrBI CCG'CTC

Cuts at:      0     526     735 
   Size:        526     209
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     547     593     735 
   Size:        547      46     142

  Fragments arranged by size: 

                 547     142      46
 
BstYI r'GATC_y

Cuts at:      0     402     436     735 
   Size:        402      34     299

  Fragments arranged by size: 

                 402     299      34
 
BtgI C'CryG_G

Cuts at:      0     430     568     735 
   Size:        430     138     167

  Fragments arranged by size: 

                 430     167     138
 
DraIII CAC_nnn'GTG

Cuts at:      0     166     295     735 
   Size:        166     129     440

  Fragments arranged by size: 

                 440     166     129
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     281     735 
   Size:        281     454
 
EaeI y'GGCC_r

Cuts at:      0      81     100     735 
   Size:         81      19     635

  Fragments arranged by size: 

                 635      81      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     125     227     494     668     735 
   Size:        125     102     267     174      67

  Fragments arranged by size: 

                 267     174     125     102      67
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      92     735 
   Size:         92     643
 
EcoO109I rG'GnC_Cy

Cuts at:      0     247     325     688     735 
   Size:        247      78     363      47

  Fragments arranged by size: 

                 363     247      78      47
 
HaeII r_GCGC'y

Cuts at:      0     463     735 
   Size:        463     272
 
KasI G'GCGC_C

Cuts at:      0     459     735 
   Size:        459     276
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     134     735 
   Size:        134     601
 
MscI TGG'CCA

Cuts at:      0      83     102     735 
   Size:         83      19     633

  Fragments arranged by size: 

                 633      83      19
 
MspA1I CmG'CkG

Cuts at:      0     409     415     735 
   Size:        409       6     320

  Fragments arranged by size: 

                 409     320       6
 
NarI GG'CG_CC

Cuts at:      0     460     735 
   Size:        460     275
 
NcoI C'CATG_G

Cuts at:      0     430     568     735 
   Size:        430     138     167

  Fragments arranged by size: 

                 430     167     138
 
NdeI CA'TA_TG

Cuts at:      0     387     735 
   Size:        387     348
 
PasI CC'CwG_GG

Cuts at:      0     547     735 
   Size:        547     188
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     116     295     712     735 
   Size:        116     179     417      23

  Fragments arranged by size: 

                 417     179     116      23
 
PfoI T'CCnGG_A

Cuts at:      0     243     735 
   Size:        243     492
 
PpuMI rG'GwC_Cy

Cuts at:      0     247     688     735 
   Size:        247     441      47

  Fragments arranged by size: 

                 441     247      47
 
PspOMI G'GGCC_C

Cuts at:      0     324     735 
   Size:        324     411
 
PstI C_TGCA'G

Cuts at:      0     414     735 
   Size:        414     321
 
PvuII CAG'CTG

Cuts at:      0     409     415     735 
   Size:        409       6     320

  Fragments arranged by size: 

                 409     320       6
 
SexAI A'CCwGG_T

Cuts at:      0     291     735 
   Size:        291     444
 
SfcI C'TryA_G

Cuts at:      0     410     623     735 
   Size:        410     213     112

  Fragments arranged by size: 

                 410     213     112
 
SfoI GGC'GCC

Cuts at:      0     461     735 
   Size:        461     274
 
SmlI C'TyrA_G

Cuts at:      0      92     735 
   Size:         92     643
 
StyI C'CwwG_G

Cuts at:      0     115     430     454     568     735 
   Size:        115     315      24     114     167

  Fragments arranged by size: 

                 315     167     115     114      24
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     411     735 
   Size:        411     324
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     566     590     735 
   Size:        566      24     145

  Fragments arranged by size: 

                 566     145      24
 

 Enzymes that do cut:

     AcuI     ApaI    ApaLI     ApoI    BamHI     BanI    BanII     BbeI
     BbsI    BciVI     BglI Bme1580I     BmrI     BpmI    BpuEI    BsaHI
    BsaWI    BseRI    BseYI  BsiHKAI Bsp1286I    BspEI    BsrBI    BstXI
    BstYI     BtgI   DraIII     DrdI     EaeI  Eco57MI    EcoNI EcoO109I
    HaeII     KasI     MmeI     MscI   MspA1I     NarI     NcoI     NdeI
     PasI    PflMI     PfoI    PpuMI   PspOMI     PstI    PvuII    SexAI
     SfcI     SfoI     SmlI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BbvCI     BcgI     BclI    BfrBI
    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspHI
    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
     EagI     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MslI     NaeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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