Restriction map for pF1KB4223
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

Acc65I      AccI        AclI        AflII       AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AvaI        AviII       AvrII       
BamHI       BanII       BbrPI       BbsI        BcgI        
BciVI       BfaI        BfrI        BglI        BlnI        
BmyI        BpuAI       BsaAI       BsaBI       BsaI        
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspHI       
BspLU11I    BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraII       DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoT22I     FseI        FspI        HindIII     
HinfI       HpaI        KpnI        KspI        MaeI        
MamI        MfeI        MunI        NaeI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        SexAI       SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AatII        1039  GACGTC               GACGT^C
AccIII        969  TCCGGA               T^CCGGA
AlwI          123  GATCC                GGATC(4/5)
AspI          450  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1094  TGGCCA               TGG^CCA
BanI           39  GGCGCC               G^G[CT][GA]CC
BbeI           39  GGCGCC               GGCGC^C
BclI          339  TGATCA               T^GATCA
BglII        1181  AGATCT               A^GATCT
BpmI          781  CTGGAG               CTGGAG(16/14)
BsaWI         969  TCCGGA               [AT]^CCGG[AT]
BseAI         969  TCCGGA               T^CCGGA
BsiEI         429  CGACCG               CG[GA][CT]^CG
BsmFI         797  GGGAC                GGGAC(10/14)
BspEI         969  TCCGGA               T^CCGGA
BspMI         263  ACCTGC               ACCTGC(4/8)
BsrDI         173  GCAATG               GCAATG(2/0)
BsrFI         431  ACCGGC               [GA]^CCGG[CT]
BsrI          504  CCAGT                ACTGG(1/-1)
BstEII        597  GGTTACC              G^GT[ACGT]ACC
BstPI         597  GGTTACC              G^GT[ACGT]ACC
BstXI          23  CCACGGCGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1181  AGATCT               [GA]^GATC[CT]
Cfr10I        431  ACCGGC               [GA]^CCGG[CT]
DraIII        856  CACGACGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         1094  TGGCCA               [CT]^GGCC[GA]
EarI          247  CTCTTC               CTCTTC(1/4)
EcoO65I       597  GGTTACC              G^GT[ACGT]ACC
EcoRV        1105  GATATC               GAT^ATC
FbaI          339  TGATCA               T^GATCA
HincII       1036  GTTGAC               GT[CT]^[GA]AC
HindII       1036  GTTGAC               GT[CT]^[GA]AC
KasI           39  GGCGCC               G^GCGCC
Ksp632I       247  CTCTTC               CTCTTC(1/4)
MflI         1181  AGATCT               [GA]^GATC[CT]
MluI          409  ACGCGT               A^CGCGT
MluNI        1094  TGGCCA               TGG^CCA
MroI          969  TCCGGA               T^CCGGA
MscI         1094  TGGCCA               TGG^CCA
MspA1I        817  CAGCTG               C[AC]G^C[GT]G
NarI           39  GGCGCC               GG^CGCC
NcoI          299  CCATGG               C^CATGG
PflFI         450  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         855  CCACGACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII         817  CAGCTG               CAG^CTG
ScaI         1205  AGTACT               AGT^ACT
SfoI           39  GGCGCC               GGC^GCC
SmlI          649  CTCAAG               C^T[CT][GA]AG
Tth111I       450  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        855  CCACGACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII        1181  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccII         365  CGCG                 CG^CG
              410  CGCG                
AcsI          354  GAATTC               [GA]^AATT[CT]
             1021  GAATTC              
AcyI           39  GGCGCC               G[GA]^CG[CT]C
             1039  GACGTC              
AfaI          558  GTAC                 GT^AC
             1206  GTAC                
AflIII        409  ACGCGT               A^C[GA][CT]GT
              608  ACGTGT              
AlwNI         544  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              742  CAGAAGCTG           
ApoI          354  GAATTC               [GA]^AATT[CT]
             1021  GAATTC              
AvaII         864  GGACC                G^G[AT]CC
             1012  GGTCC               
BbvCI         126  CCTCAGC              CCTCAGC(-5/-2)
              990  CCTCAGC             
BcnI          108  CCGGG                CC^[GC]GG
              867  CCGGG               
BlpI           45  GCTCAGC              GC^T[ACGT]AGC
              168  GCTGAGC             
Bpu1102I       45  GCTCAGC              GC^T[ACGT]AGC
              168  GCTGAGC             
BsaHI          39  GGCGCC               G[GA]^CG[CT]C
             1039  GACGTC              
BsgI           12  CTGCAC               GTGCAG(16/14)
             1139  CTGCAC              
BsrBI          43  CCGCTC               CCGCTC(-3/-3)
              898  GAGCGG              
BstAPI        741  GCAGAAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1141  GCACATGCTGC         
BstUI         365  CGCG                 CG^CG
              410  CGCG                
CelII          45  GCTCAGC              GC^T[ACGT]AGC
              168  GCTGAGC             
EcoRI         354  GAATTC               G^AATTC
             1021  GAATTC              
EcoT14I       299  CCATGG               C^C[AT][AT]GG
              695  CCAAGG              
FauI          795  GCGGG                CCCGC(4/6)
              900  GCGGG               
HgaI          363  GACGC                GACGC(5/10)
              131  GCGTC               
Hin1I          39  GGCGCC               G[GA]^CG[CT]C
             1039  GACGTC              
MslI           24  CACGGCGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              192  CATGCTAATG          
MvnI          365  CGCG                 CG^CG
              410  CGCG                
NciI          108  CCGGG                CC^[GC]GG
              867  CCGGG               
NlaIV          39  GGCGCC               GG[ACGT]^[ACGT]CC
             1174  GGGGCC              
RsaI          558  GTAC                 GT^AC
             1206  GTAC                
SfcI          219  CTACAG               C^T[GA][CT]AG
              834  CTACAG              
StyI          299  CCATGG               C^C[AT][AT]GG
              695  CCAAGG              
TaqI         1025  TCGA                 T^CGA
             1186  TCGA                
Tsp45I        361  GTGAC                ^GT[GC]AC
              386  GTGAC               
TthHB8I      1025  TCGA                 T^CGA
             1186  TCGA                
XcmI          393  CCAGGGTCGGTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              458  CCAGCGAGGACGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AciI        AluI        Asp700I     BbvI        BsaJI       
BseRI       BsiYI       BslI        BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DsaI        EcoRII      Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MspI        
MvaI        MwoI        NdeII       NlaIII      NspI        
Sau3AI      Sau96I      ScrFI       SfaNI       Tru9I       
TseI        Tsp509I     TspRI       XmnI        
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