Restriction map for pF1KB4120
 (Linear) (Six-base) MAPSORT of: KIBB4120.seq  Check: 156  from: 1  to: 1353



KIBB4120.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     387    1353 
   Size:        387     966
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     510     542    1353 
   Size:        510      32     811

  Fragments arranged by size: 

                 811     510      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      75     225     835    1353 
   Size:         75     150     610     518

  Fragments arranged by size: 

                 610     518     150      75
 
ApaI G_GGCC'C

Cuts at:      0    1158    1353 
   Size:       1158     195
 
ApoI r'AATT_y

Cuts at:      0     987    1353 
   Size:        987     366
 
AvaI C'yCGr_G

Cuts at:      0      80     822    1353 
   Size:         80     742     531

  Fragments arranged by size: 

                 742     531      80
 
BamHI G'GATC_C

Cuts at:      0    1143    1353 
   Size:       1143     210
 
BanII G_rGCy'C

Cuts at:      0     829    1158    1353 
   Size:        829     329     195

  Fragments arranged by size: 

                 829     329     195
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     254    1353 
   Size:        254    1099
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     853     887    1353 
   Size:        853      34     466

  Fragments arranged by size: 

                 853     466      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     904    1353 
   Size:        904     449
 
BfrBI ATG'CAT

Cuts at:      0     199    1353 
   Size:        199    1154
 
BlpI GC'TnA_GC

Cuts at:      0     828     950    1353 
   Size:        828     122     403

  Fragments arranged by size: 

                 828     403     122
 
Bme1580I G_kGCm'C

Cuts at:      0    1158    1353 
   Size:       1158     195
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     583     837    1353 
   Size:        583     254     516

  Fragments arranged by size: 

                 583     516     254
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202    1353 
   Size:        202    1151
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     139     696    1353 
   Size:        139     557     657

  Fragments arranged by size: 

                 657     557     139
 
BsaBI GATnn'nnATC

Cuts at:      0    1142    1353 
   Size:       1142     211
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      22      52     510     540    1353 
   Size:         22      30     458      30     813

  Fragments arranged by size: 

                 813     458      30      30      22
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      40      43     157    1114    1353 
   Size:         40       3     114     957     239

  Fragments arranged by size: 

                 957     239     114      40       3
 
BsmI GAATG_Cn'

Cuts at:      0     773    1353 
   Size:        773     580
 
Bsp1286I G_dGCh'C

Cuts at:      0     829    1158    1353 
   Size:        829     329     195

  Fragments arranged by size: 

                 829     329     195
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     387    1353 
   Size:        387     966
 
BsrDI GCAATG_nn'

Cuts at:      0     779    1353 
   Size:        779     574
 
BstYI r'GATC_y

Cuts at:      0      32    1143    1353 
   Size:         32    1111     210

  Fragments arranged by size: 

                1111     210      32
 
BtgI C'CryG_G

Cuts at:      0     450    1353 
   Size:        450     903
 
ClaI AT'CG_AT

Cuts at:      0     422    1353 
   Size:        422     931
 
DraI TTT'AAA

Cuts at:      0     695    1353 
   Size:        695     658
 
DraIII CAC_nnn'GTG

Cuts at:      0     718    1353 
   Size:        718     635
 
EaeI y'GGCC_r

Cuts at:      0     203    1353 
   Size:        203    1150
 
EarI CTCTTCn'nnn_

Cuts at:      0     312    1353 
   Size:        312    1041
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202    1353 
   Size:        202    1151
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1155    1353 
   Size:       1155     198
 
EcoRV GAT'ATC

Cuts at:      0     231     869    1353 
   Size:        231     638     484

  Fragments arranged by size: 

                 638     484     231
 
HaeII r_GCGC'y

Cuts at:      0      55     396    1353 
   Size:         55     341     957

  Fragments arranged by size: 

                 957     341      55
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      22      54     507     539    1207    1239    1353 
   Size:         22      32     453      32     668      32     114

  Fragments arranged by size: 

                 668     453     114      32      32      32      22
 
HindIII A'AGCT_T

Cuts at:      0     134    1353 
   Size:        134    1219
 
MfeI C'AATT_G

Cuts at:      0     992    1353 
   Size:        992     361
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1030    1353 
   Size:       1030     323
 
MscI TGG'CCA

Cuts at:      0     205    1353 
   Size:        205    1148
 
MslI CAynn'nnrTG

Cuts at:      0     784     962    1042    1353 
   Size:        784     178      80     311

  Fragments arranged by size: 

                 784     311     178      80
 
NcoI C'CATG_G

Cuts at:      0     450    1353 
   Size:        450     903
 
NdeI CA'TA_TG

Cuts at:      0    1131    1353 
   Size:       1131     222
 
NsiI A_TGCA'T

Cuts at:      0     201    1353 
   Size:        201    1152
 
PasI CC'CwG_GG

Cuts at:      0    1315    1353 
   Size:       1315      38
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     225    1353 
   Size:        225    1128
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     510     542    1353 
   Size:        510      32     811

  Fragments arranged by size: 

                 811     510      32
 
PsiI TTA'TAA

Cuts at:      0     593    1255    1353 
   Size:        593     662      98

  Fragments arranged by size: 

                 662     593      98
 
PspOMI G'GGCC_C

Cuts at:      0    1154    1353 
   Size:       1154     199
 
PstI C_TGCA'G

Cuts at:      0      14    1110    1353 
   Size:         14    1096     243

  Fragments arranged by size: 

                1096     243      14
 
SfcI C'TryA_G

Cuts at:      0      10     119    1106    1353 
   Size:         10     109     987     247

  Fragments arranged by size: 

                 987     247     109      10
 
SmaI CCC'GGG

Cuts at:      0     824    1353 
   Size:        824     529
 
StyI C'CwwG_G

Cuts at:      0     450     706    1353 
   Size:        450     256     647

  Fragments arranged by size: 

                 647     450     256
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     819    1353 
   Size:        819     534
 
TatI w'GTAC_w

Cuts at:      0     213    1353 
   Size:        213    1140
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     702     734    1353 
   Size:        702      32     619

  Fragments arranged by size: 

                 702     619      32
 
XmaI C'CCGG_G

Cuts at:      0     822    1353 
   Size:        822     531
 

 Enzymes that do cut:

     AarI     AloI    AlwNI     ApaI     ApoI     AvaI    BamHI    BanII
     BbsI     BcgI    BciVI    BfrBI     BlpI Bme1580I     BmrI     BpmI
     BsaI    BsaBI    BsaXI    BseRI     BsmI Bsp1286I    BspMI    BsrDI
    BstYI     BtgI     ClaI     DraI   DraIII     EaeI     EarI  Eco57MI
 EcoO109I    EcoRV    HaeII    Hin4I  HindIII     MfeI     MmeI     MscI
     MslI     NcoI     NdeI     NsiI     PasI    PflMI     PpiI     PsiI
   PspOMI     PstI     SfcI     SmaI     StyI    TaqII     TatI     TstI
     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI     BanI     BbeI    BbvCI     BclI     BglI    BglII
    BmgBI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI    CspCI     DrdI     EagI
     EciI  EcoICRI    EcoNI    EcoRI     FalI     FseI     FspI    FspAI
   HincII     HpaI     KasI     KpnI     MluI   MspA1I     NaeI     NarI
   NgoMIV     NheI     NotI     NruI     NspI     PacI     PciI     PfoI
     PmeI     PmlI    PpuMI    PshAI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI     XcmI
     XhoI     XmnI     ZraI
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