Restriction map for pF1KB4017
##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      AlwI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AviII       AvrII       
BamHI       BbrPI       BbvCI       BciVI       BfrI        
BlnI        BsaAI       BsaBI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspHI       BspLU11I    
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        DraI        DraIII      
EagI        Eam1105I    EclXI       Eco52I      EcoRI       
EcoRV       EcoT22I     FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MamI        MluI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        ScaI        SexAI       
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SspBI       SspI        SwaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccIII       1043  TCCGGA               T^CCGGA
AflIII       1085  ACGTGT               A^C[GA][CT]GT
AocI         1014  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       578  AGCGCT               AGC^GCT
ApaI           81  GGGCCC               GGGCC^C
AspI          115  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI          101  GGCGCC               GGCGC^C
BcgI          807  CGACTTCCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1307  TGATCA               T^GATCA
BfaI           37  CTAG                 C^TAG
BlpI          688  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      688  GCTGAGC              GC^T[ACGT]AGC
BsaI           43  GAGACC               GGTCTC(1/5)
BsaWI        1043  TCCGGA               [AT]^CCGG[AT]
BseAI        1043  TCCGGA               T^CCGGA
BsmAI          43  GAGAC                GTCTC(1/5)
BspEI        1043  TCCGGA               T^CCGGA
BsrBI          51  CCGCTC               CCGCTC(-3/-3)
BsrDI         478  CATTGC               GCAATG(2/0)
Bsu36I       1014  CCTCAGG              CC^T[ACGT]AGG
CelII         688  GCTGAGC              GC^T[ACGT]AGC
DsaI           93  CCACGG               C^C[GA][CT]GG
Eco47III      578  AGCGCT               AGC^GCT
Eco81I       1014  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1315  CCTAACTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI         1307  TGATCA               T^GATCA
HgaI         1140  GACGC                GACGC(5/10)
KasI          101  GGCGCC               G^GCGCC
MaeI           37  CTAG                 C^TAG
MfeI          819  CAATTG               C^AATTG
MroI         1043  TCCGGA               T^CCGGA
MseI         1252  TTAA                 T^TAA
MunI          819  CAATTG               C^AATTG
NaeI          698  GCCGGC               GCC^GGC
NarI          101  GGCGCC               GG^CGCC
NgoMIV        698  GCCGGC               G^CCGGC
PflFI         115  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         477  CCATTGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          931  GACTC                GAGTC(4/5)
PshAI        1185  GACCAGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI          657  GCTCTTC              GCTCTTC(1/4)
SbfI           75  CCTGCAGG             CCTGCA^GG
SfiI         1113  GGCCACCCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          101  GGCGCC               GGC^GCC
SphI          653  GCATGC               GCATG^C
Sse8387I       75  CCTGCAGG             CCTGCA^GG
TfiI          148  GAATC                G^A[AT]TC
Tru9I        1252  TTAA                 T^TAA
Tth111I       115  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        477  CCATTGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI           36  TCTAGA               T^CTAGA
XcmI          941  CCACTGGCATCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   44 enzymes) #
###################################

AccII         199  CGCG                 CG^CG
              559  CGCG                
AcyI          101  GGCGCC               G[GA]^CG[CT]C
             1140  GACGCC              
AspHI          88  GAGCTC               G[AT]GC[AT]^C
             1270  GAGCTC              
AvaI           85  CCCGAG               C^[CT]CG[GA]G
             1127  CTCGGG              
BalI           59  TGGCCA               TGG^CCA
              218  TGGCCA              
BanI          101  GGCGCC               G^G[CT][GA]CC
              630  GGTGCC              
BbsI         1137  GAAGAC               GAAGAC(2/6)
               16  GTCTTC              
BglII         320  AGATCT               A^GATCT
              563  AGATCT              
BpmI          933  CTCCAG               CTGGAG(16/14)
              327  CTGGAG              
BpuAI        1137  GAAGAC               GAAGAC(2/6)
               16  GTCTTC              
BsaHI         101  GGCGCC               G[GA]^CG[CT]C
             1140  GACGCC              
BsgI         1203  CTGCAC               GTGCAG(16/14)
              966  GTGCAG              
BsiEI         201  CGACCG               CG[GA][CT]^CG
              624  CGACCG              
BsiHKAI        88  GAGCTC               G[AT]GC[AT]^C
             1270  GAGCTC              
BsoBI          85  CCCGAG               C^[CT]CG[GA]G
             1127  CTCGGG              
BspMI         758  ACCTGC               ACCTGC(4/8)
             1201  ACCTGC              
BsrFI         387  GCCGGT               [GA]^CCGG[CT]
              698  GCCGGC              
BstAPI        309  GCAGTGTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              473  GCAACCATTGC         
BstEII        390  GGTGACC              G^GT[ACGT]ACC
              843  GGTCACC             
BstPI         390  GGTGACC              G^GT[ACGT]ACC
              843  GGTCACC             
BstUI         199  CGCG                 CG^CG
              559  CGCG                
BstXI         746  CCAACTGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1056  CCAGGCCTTTGG        
BstYI         320  AGATCT               [GA]^GATC[CT]
              563  AGATCT              
Cfr10I        387  GCCGGT               [GA]^CCGG[CT]
              698  GCCGGC              
DrdI          451  GACCCGGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1356  GACCGTGCTGTC        
EarI           25  CTCTTC               CTCTTC(1/4)
              658  CTCTTC              
EcoO65I       390  GGTGACC              G^GT[ACGT]ACC
              843  GGTCACC             
EcoT14I       242  CCTTGG               C^C[AT][AT]GG
              494  CCAAGG              
Hin1I         101  GGCGCC               G[GA]^CG[CT]C
             1140  GACGCC              
HinfI         148  GAATC                G^A[ACGT]TC
              931  GACTC               
Ksp632I        25  CTCTTC               CTCTTC(1/4)
              658  CTCTTC              
MflI          320  AGATCT               [GA]^GATC[CT]
              563  AGATCT              
MluNI          59  TGGCCA               TGG^CCA
              218  TGGCCA              
MscI           59  TGGCCA               TGG^CCA
              218  TGGCCA              
MslI          948  CATCGTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1074  CACGGACGTG          
MvnI          199  CGCG                 CG^CG
              559  CGCG                
NspI          653  GCATGC               [GA]CATG^[CT]
             1256  GCATGT              
SacI           88  GAGCTC               GAGCT^C
             1270  GAGCTC              
StuI          497  AGGCCT               AGG^CCT
             1058  AGGCCT              
StyI          242  CCTTGG               C^C[AT][AT]GG
              494  CCAAGG              
TaqI          623  TCGA                 T^CGA
              825  TCGA                
Tsp509I       820  AATT                 ^AATT
              865  AATT                
TthHB8I       623  TCGA                 T^CGA
              825  TCGA                
XhoII         320  AGATCT               [GA]^GATC[CT]
              563  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       AvaII       
BanII       BbvI        BcnI        BglI        BmyI        
BsaJI       BseRI       BsiYI       BslI        BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TseI        Tsp45I      TspRI       
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