Restriction map for pF1KB4017
 (Linear) (Six-base) MAPSORT of: KIBB4017.seq  Check: 8748  from: 1  to: 1392



KIBB4017.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1210    1392 
   Size:       1210     182
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     399    1249    1392 
   Size:        399     850     143

  Fragments arranged by size: 

                 850     399     143
 
AfeI AGC'GCT

Cuts at:      0     580    1392 
   Size:        580     812
 
AflIII A'CryG_T

Cuts at:      0    1085    1392 
   Size:       1085     307
 
AleI CACnn'nnGTG

Cuts at:      0    1078    1392 
   Size:       1078     314
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     229     544    1392 
   Size:        229     315     848

  Fragments arranged by size: 

                 848     315     229
 
AlwNI CAG_nnn'CTG

Cuts at:      0     189     315    1227    1392 
   Size:        189     126     912     165

  Fragments arranged by size: 

                 912     189     165     126
 
ApaI G_GGCC'C

Cuts at:      0      85    1392 
   Size:         85    1307
 
AvaI C'yCGr_G

Cuts at:      0      85    1127    1392 
   Size:         85    1042     265

  Fragments arranged by size: 

                1042     265      85
 
BanI G'GyrC_C

Cuts at:      0     101     630    1392 
   Size:        101     529     762

  Fragments arranged by size: 

                 762     529     101
 
BanII G_rGCy'C

Cuts at:      0      85      92     265     886    1274    1392 
   Size:         85       7     173     621     388     118

  Fragments arranged by size: 

                 621     388     173     118      85       7
 
BbeI G_GCGC'C

Cuts at:      0     105    1392 
   Size:        105    1287
 
BbsI GAAGACnn'nnnn_

Cuts at:      0       9    1144    1392 
   Size:          9    1135     248

  Fragments arranged by size: 

                1135     248       9
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     796     830    1392 
   Size:        796      34     562

  Fragments arranged by size: 

                 796     562      34
 
BclI T'GATC_A

Cuts at:      0    1307    1392 
   Size:       1307      85
 
BglI GCCn_nnn'nGGC

Cuts at:      0     194     944    1057    1120    1392 
   Size:        194     750     113      63     272

  Fragments arranged by size: 

                 750     272     194     113      63
 
BglII A'GATC_T

Cuts at:      0     320     563    1392 
   Size:        320     243     829

  Fragments arranged by size: 

                 829     320     243
 
BlpI GC'TnA_GC

Cuts at:      0     689    1392 
   Size:        689     703
 
Bme1580I G_kGCm'C

Cuts at:      0      85     635    1392 
   Size:         85     550     757

  Fragments arranged by size: 

                 757     550      85
 
BmgBI CAC'GTC

Cuts at:      0    1080    1208    1392 
   Size:       1080     128     184

  Fragments arranged by size: 

                1080     184     128
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     103    1392 
   Size:        103    1289
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     348     918    1392 
   Size:        348     570     474

  Fragments arranged by size: 

                 570     474     348
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      37    1392 
   Size:         37    1355
 
BsaHI Gr'CG_yC

Cuts at:      0     102    1141    1392 
   Size:        102    1039     251

  Fragments arranged by size: 

                1039     251     102
 
BsaWI w'CCGG_w

Cuts at:      0    1043    1392 
   Size:       1043     349
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     295     325     358     388     985    1015    1392 
   Size:        295      30      33      30     597      30     377

  Fragments arranged by size: 

                 597     377     295      33      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     373     525    1009    1012    1392 
   Size:        373     152     484       3     380

  Fragments arranged by size: 

                 484     380     373     152       3
 
BseYI C'CCAG_C

Cuts at:      0     634     715    1327    1392 
   Size:        634      81     612      65

  Fragments arranged by size: 

                 634     612      81      65
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     987    1188    1392 
   Size:        987     201     204

  Fragments arranged by size: 

                 987     204     201
 
BsiEI CG_ry'CG

Cuts at:      0     204     627    1392 
   Size:        204     423     765

  Fragments arranged by size: 

                 765     423     204
 
BsiHKAI G_wGCw'C

Cuts at:      0      92    1274    1392 
   Size:         92    1182     118

  Fragments arranged by size: 

                1182     118      92
 
Bsp1286I G_dGCh'C

Cuts at:      0      85      92     265     635     886    1274    1392 
   Size:         85       7     173     370     251     388     118

  Fragments arranged by size: 

                 388     370     251     173     118      85       7
 
BspEI T'CCGG_A

Cuts at:      0    1043    1392 
   Size:       1043     349
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     767    1210    1392 
   Size:        767     443     182

  Fragments arranged by size: 

                 767     443     182
 
BsrBI CCG'CTC

Cuts at:      0      53    1392 
   Size:         53    1339
 
BsrDI GCAATG_nn'

Cuts at:      0     477    1392 
   Size:        477     915
 
BsrFI r'CCGG_y

Cuts at:      0     387     698    1392 
   Size:        387     311     694

  Fragments arranged by size: 

                 694     387     311
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     315     479    1392 
   Size:        315     164     913

  Fragments arranged by size: 

                 913     315     164
 
BstEII G'GTnAC_C

Cuts at:      0     390     843    1392 
   Size:        390     453     549

  Fragments arranged by size: 

                 549     453     390
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     753    1063    1392 
   Size:        753     310     329

  Fragments arranged by size: 

                 753     329     310
 
BstYI r'GATC_y

Cuts at:      0     320     563    1392 
   Size:        320     243     829

  Fragments arranged by size: 

                 829     320     243
 
Bsu36I CC'TnA_GG

Cuts at:      0    1015    1392 
   Size:       1015     377
 
BtgI C'CryG_G

Cuts at:      0      93    1392 
   Size:         93    1299
 
BtsI GCAGTG_nn'

Cuts at:      0     190     316    1392 
   Size:        190     126    1076

  Fragments arranged by size: 

                1076     190     126
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     457    1362    1392 
   Size:        457     905      30

  Fragments arranged by size: 

                 905     457      30
 
EaeI y'GGCC_r

Cuts at:      0      59     218     803    1392 
   Size:         59     159     585     589

  Fragments arranged by size: 

                 589     585     159      59
 
EarI CTCTTCn'nnn_

Cuts at:      0      31     664    1392 
   Size:         31     633     728

  Fragments arranged by size: 

                 728     633      31
 
EcoICRI GAG'CTC

Cuts at:      0      90    1272    1392 
   Size:         90    1182     120

  Fragments arranged by size: 

                1182     120      90
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     348     399     918    1249    1392 
   Size:        348      51     519     331     143

  Fragments arranged by size: 

                 519     348     331     143      51
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1319    1392 
   Size:       1319      73
 
EcoO109I rG'GnC_Cy

Cuts at:      0      81      82     606    1099    1122    1324    1392 
   Size:         81       1     524     493      23     202      68

  Fragments arranged by size: 

                 524     493     202      81      68      23       1
 
HaeII r_GCGC'y

Cuts at:      0     105     467     582     696    1392 
   Size:        105     362     115     114     696

  Fragments arranged by size: 

                 696     362     115     114     105
 
KasI G'GCGC_C

Cuts at:      0     101    1392 
   Size:        101    1291
 
MfeI C'AATT_G

Cuts at:      0     819    1392 
   Size:        819     573
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     176    1392 
   Size:        176    1216
 
MscI TGG'CCA

Cuts at:      0      61     220    1392 
   Size:         61     159    1172

  Fragments arranged by size: 

                1172     159      61
 
MslI CAynn'nnrTG

Cuts at:      0     952    1078    1392 
   Size:        952     126     314

  Fragments arranged by size: 

                 952     314     126
 
MspA1I CmG'CkG

Cuts at:      0     267     281     719     851     971     983    1392 
   Size:        267      14     438     132     120      12     409

  Fragments arranged by size: 

                 438     409     267     132     120      14      12
 
NaeI GCC'GGC

Cuts at:      0     700    1392 
   Size:        700     692
 
NarI GG'CG_CC

Cuts at:      0     102    1392 
   Size:        102    1290
 
NgoMIV G'CCGG_C

Cuts at:      0     698    1392 
   Size:        698     694
 
NspI r_CATG'y

Cuts at:      0     657    1260    1392 
   Size:        657     603     132

  Fragments arranged by size: 

                 657     603     132
 
PasI CC'CwG_GG

Cuts at:      0    1291    1392 
   Size:       1291     101
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     483    1392 
   Size:        483     909
 
PfoI T'CCnGG_A

Cuts at:      0    1214    1392 
   Size:       1214     178
 
PshAI GACnn'nnGTC

Cuts at:      0    1189    1392 
   Size:       1189     203
 
PspOMI G'GGCC_C

Cuts at:      0      81    1392 
   Size:         81    1311
 
PstI C_TGCA'G

Cuts at:      0      80     160     311     320    1392 
   Size:         80      80     151       9    1072

  Fragments arranged by size: 

                1072     151      80      80       9
 
PvuII CAG'CTG

Cuts at:      0     719     851     971    1392 
   Size:        719     132     120     421

  Fragments arranged by size: 

                 719     421     132     120
 
SacI G_AGCT'C

Cuts at:      0      92    1274    1392 
   Size:         92    1182     118

  Fragments arranged by size: 

                1182     118      92
 
SapI GCTCTTCn'nnn_

Cuts at:      0     664    1392 
   Size:        664     728
 
SbfI CC_TGCA'GG

Cuts at:      0      80    1392 
   Size:         80    1312
 
SfcI C'TryA_G

Cuts at:      0      76     156     307     316    1392 
   Size:         76      80     151       9    1076

  Fragments arranged by size: 

                1076     151      80      76       9
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1120    1392 
   Size:       1120     272
 
SfoI GGC'GCC

Cuts at:      0     103    1392 
   Size:        103    1289
 
SphI G_CATG'C

Cuts at:      0     657    1392 
   Size:        657     735
 
StuI AGG'CCT

Cuts at:      0     499    1060    1392 
   Size:        499     561     332

  Fragments arranged by size: 

                 561     499     332
 
StyI C'CwwG_G

Cuts at:      0     242     494    1392 
   Size:        242     252     898

  Fragments arranged by size: 

                 898     252     242
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     364     782    1132    1392 
   Size:        364     418     350     260

  Fragments arranged by size: 

                 418     364     350     260
 
TatI w'GTAC_w

Cuts at:      0     641    1392 
   Size:        641     751
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     295     327     985    1017    1392 
   Size:        295      32     658      32     375

  Fragments arranged by size: 

                 658     375     295      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     118    1392 
   Size:        118    1274
 
XbaI T'CTAG_A

Cuts at:      0      36    1392 
   Size:         36    1356
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     948    1392 
   Size:        948     444
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AleI     AlfI    AlwNI     ApaI
     AvaI     BanI    BanII     BbeI     BbsI     BcgI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BpmI     BsaI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BstAPI   BstEII    BstXI
    BstYI   Bsu36I     BtgI     BtsI     DrdI     EaeI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I    HaeII     KasI     MfeI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PasI    PflMI
     PfoI    PshAI   PspOMI     PstI    PvuII     SacI     SapI     SbfI
     SfcI     SfiI     SfoI     SphI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AloI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BbvCI    BciVI    BfrBI     BmtI     BplI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsiWI     BsmI    BsmBI    BspHI    BsrGI   BssHII    BssSI
    BstBI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     EagI
     EciI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KpnI     MluI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI     PpiI
    PpuMI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     ScaI    SexAI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SrfI     SspI     SwaI     XhoI     XmaI     XmnI     ZraI
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