Restriction map for pF1KB3981
##################################
# NO CUT ENZYMES (  176 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        AocI        Aor51HI     ApaI        ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AvaI        AvaII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsiWI       BsmI        BsoBI       BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
Cfr10I      Cfr13I      ClaI        CpoI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
HaeII       HaeIII      HapII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        HpaII       
KasI        KpnI        KspI        MaeII       MaeIII      
MamI        MfeI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MspI        MunI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
StyI        SwaI        TfiI        Tsp45I      Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccII         445  CGCG                 CG^CG
AfaI          166  GTAC                 GT^AC
Alw44I         35  GTGCAC               G^TGCAC
AlwI          462  GATCC                GGATC(4/5)
AlwNI         398  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI          35  GTGCAC               G^TGCAC
AspI          448  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII          20  TGCGCA               TGC^GCA
BbsI          118  GAAGAC               GAAGAC(2/6)
BfaI          319  CTAG                 C^TAG
BlpI          473  GCTCAGC              GC^T[ACGT]AGC
BpmI          331  CTGGAG               CTGGAG(16/14)
Bpu1102I      473  GCTCAGC              GC^T[ACGT]AGC
BpuAI         118  GAAGAC               GAAGAC(2/6)
BsaJI          66  CCTGGG               C^C[ACGT][ACGT]GG
BsgI          281  GTGCAG               GTGCAG(16/14)
BsiEI         447  CGACCG               CG[GA][CT]^CG
BsmAI         304  GAGAC                GTCTC(1/5)
BsmBI         304  GAGACG               CGTCTC(1/5)
BsmFI         197  GGGAC                GGGAC(10/14)
Bsp1407I      165  TGTACA               T^GTACA
BspMI         431  ACCTGC               ACCTGC(4/8)
BsrGI         165  TGTACA               T^GTACA
BstUI         445  CGCG                 CG^CG
BstYI         461  AGATCC               [GA]^GATC[CT]
CelII         473  GCTCAGC              GC^T[ACGT]AGC
CfoI           21  GCGC                 GCG^C
DpnII         462  GATC                 ^GATC
DraI          181  TTTAAA               TTT^AAA
FspI           20  TGCGCA               TGC^GCA
HhaI           21  GCGC                 GCG^C
HinP1I         21  GCGC                 G^CGC
HinfI         452  GAGTC                G^A[ACGT]TC
HphI           51  GGTGA                GGTGA(8/7)
MaeI          319  CTAG                 C^TAG
MboI          462  GATC                 ^GATC
MflI          461  AGATCC               [GA]^GATC[CT]
MseI          182  TTAA                 T^TAA
MvnI          445  CGCG                 CG^CG
NdeII         462  GATC                 ^GATC
NlaIII        507  CATG                 CATG^
PflFI         448  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          452  GAGTC                GAGTC(4/5)
RsaI          166  GTAC                 GT^AC
Sau3AI        462  GATC                 ^GATC
SfaNI         112  GCATC                GCATC(5/9)
SspBI         165  TGTACA               T^GTACA
TaqI          275  TCGA                 T^CGA
Tru9I         182  TTAA                 T^TAA
Tth111I       448  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       275  TCGA                 T^CGA
XhoII         461  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AciI          444  CCGC                 CCGC(-3/-1)
              322  GCGG                
AluI          107  AGCT                 AG^CT
              477  AGCT                
AspHI          35  GTGCAC               G[AT]GC[AT]^C
              361  GTGCTC              
BbvI          105  GCAGC                GCAGC(8/12)
              397  GCAGC               
BmyI           35  GTGCAC               G[AGT]GC[ACT]^C
              361  GTGCTC              
BsiHKAI        35  GTGCAC               G[AT]GC[AT]^C
              361  GTGCTC              
BsiYI          66  CCTGGGCTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              139  CCTACACAGGG         
BslI           66  CCTGGGCTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              139  CCTACACAGGG         
Bsp1286I       35  GTGCAC               G[AGT]GC[ACT]^C
              361  GTGCTC              
BssKI          45  CCAGG                ^CC[ACGT]GG
               66  CCTGG               
BstAPI        381  GCAGGATTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              397  GCAGCCCCTGC         
BstF5I        441  CATCC                GGATG(2/0)
              501  GGATG               
BstNI          45  CCAGG                CC^[AT]GG
               66  CCTGG               
DdeI          346  CTAAG                C^T[ACGT]AG
              474  CTCAG               
EarI           95  CTCTTC               CTCTTC(1/4)
              301  GAAGAG              
EcoRII         45  CCAGG                ^CC[AT]GG
               66  CCTGG               
Fnu4HI        105  GCAGC                GC^[ACGT]GC
              397  GCAGC               
FokI          441  CATCC                GGATG(9/13)
              501  GGATG               
ItaI          105  GCAGC                GC^[ACGT]GC
              397  GCAGC               
Ksp632I        95  CTCTTC               CTCTTC(1/4)
              301  GAAGAG              
MvaI           45  CCAGG                CC^[AT]GG
               66  CCTGG               
MwoI          381  GCAGGATTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              397  GCAGCCCCTGC         
ScrFI          45  CCAGG                CC^[ACGT]GG
               66  CCTGG               
TseI          105  GCAGC                G^C[AT]GC
              397  GCAGC               
TspRI         279  CAGTG                CA[GC]TG[ACGT][ACGT]^
              359  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

BsrI        Cac8I       MboII       MnlI        Tsp509I     
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