Restriction map for pF1KB3738
##################################
# NO CUT ENZYMES (  118 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AgeI        AlwNI       Aor51HI     
ApaI        AscI        AseI        AsnI        Asp700I     
Asp718I     AviII       AvrII       BbsI        BciVI       
BclI        BfaI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BpuAI       BsaBI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmAI       BsmBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     CelII       
ClaI        DraIII      EagI        EarI        EclXI       
Eco47III    Eco52I      EcoO65I     FbaI        FseI        
FspI        HincII      HindII      HpaI        KpnI        
Ksp632I     KspI        MaeI        MamI        MfeI        
MluI        MroI        MslI        MunI        MvnI        
NaeI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflMI       
PinAI       PleI        PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        SacI        SacII       
SalI        SapI        ScaI        SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SspBI       
SspI        SwaI        Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

AcyI          454  GGCGCC               G[GA]^CG[CT]C
AfaI          844  GTAC                 GT^AC
AflIII        905  ACACGT               A^C[GA][CT]GT
AhdI          518  GACACAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          685  CCTCAGG              CC^T[ACGT]AGG
AspEI         518  GACACAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          433  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          296  CCCGAG               C^[CT]CG[GA]G
BamHI         482  GGATCC               G^GATCC
BanI          454  GGCGCC               G^G[CT][GA]CC
BbeI          454  GGCGCC               GGCGC^C
BbrPI         906  CACGTG               CAC^GTG
BbvCI         108  GCTGAGG              CCTCAGC(-5/-2)
BglII         668  AGATCT               A^GATCT
BsaAI         906  CACGTG               [CT]AC^GT[GA]
BsaHI         454  GGCGCC               G[GA]^CG[CT]C
BsmI          117  GCATTC               GAATGC(1/-1)
BsoBI         296  CCCGAG               C^[CT]CG[GA]G
BsrDI         580  GCAATG               GCAATG(2/0)
BsrFI         389  GCCGGT               [GA]^CCGG[CT]
Bsu36I        685  CCTCAGG              CC^T[ACGT]AGG
Cfr10I        389  GCCGGT               [GA]^CCGG[CT]
CpoI           45  CGGACCG              CG^G[AT]CCG
DraI         1012  TTTAAA               TTT^AAA
DrdI          606  GACTGATCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          194  CCATGG               C^C[GA][CT]GG
Eam1105I      518  GACACAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        685  CCTCAGG              CC^T[ACGT]AGG
EcoNI          82  CCTGAACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         310  GAATTC               G^AATTC
EcoRV         877  GATATC               GAT^ATC
EcoT22I       115  ATGCAT               ATGCA^T
FauI          775  GCGGG                CCCGC(4/6)
HaeII         454  GGCGCC               [GA]GCGC^[CT]
HgaI          934  GCGTC                GACGC(5/10)
Hin1I         454  GGCGCC               G[GA]^CG[CT]C
KasI          454  GGCGCC               G^GCGCC
MaeII         907  ACGT                 A^CGT
MseI         1013  TTAA                 T^TAA
MspA1I        715  CAGCTG               C[AC]G^C[GT]G
NarI          454  GGCGCC               GG^CGCC
NcoI          194  CCATGG               C^CATGG
NsiI          115  ATGCAT               ATGCA^T
NspI          855  GCATGC               [GA]CATG^[CT]
PflFI         433  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         906  CACGTG               CAC^GTG
PmlI          906  CACGTG               CAC^GTG
PstI          492  CTGCAG               CTGCA^G
PvuII         715  CAGCTG               CAG^CTG
RsaI          844  GTAC                 GT^AC
RsrII          45  CGGACCG              CG^G[AT]CCG
SbfI          491  CCTGCAGG             CCTGCA^GG
SexAI         261  ACCAGGT              A^CC[AT]GGT
SfcI          492  CTGCAG               C^T[GA][CT]AG
SfoI          454  GGCGCC               GGC^GCC
SphI          855  GCATGC               GCATG^C
Sse8387I      491  CCTGCAGG             CCTGCA^GG
Tru9I        1013  TTAA                 T^TAA
Tth111I       433  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          863  CCATCCGGGAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcsI          174  GAATTT               [GA]^AATT[CT]
              310  GAATTC              
Alw44I         40  GTGCAC               G^TGCAC
              747  GTGCAC              
ApaLI          40  GTGCAC               G^TGCAC
              747  GTGCAC              
ApoI          174  GAATTT               [GA]^AATT[CT]
              310  GAATTC              
BalI          572  TGGCCA               TGG^CCA
              910  TGGCCA              
BanII         681  GAGCCC               G[GA]GC[CT]^C
              825  GGGCTC              
BcgI          282  CGAGCCTGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              920  CGATTTCGATGC        
BcnI           49  CCGGG                CC^[GC]GG
              867  CCGGG               
BssSI         568  CTCGTG               CACGAG(-5/-1)
              808  CTCGTG              
BstXI         194  CCATGGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              575  CCAAAGCAATGG        
BstYI         482  GGATCC               [GA]^GATC[CT]
              668  AGATCT              
CfoI           60  GCGC                 GCG^C
              455  GCGC                
DraII         524  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              802  GGGACCC             
EcoO109I      524  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              802  GGGACCC             
HhaI           60  GCGC                 GCG^C
              455  GCGC                
HinP1I         60  GCGC                 G^CGC
              455  GCGC                
HindIII       212  AAGCTT               A^AGCTT
              424  AAGCTT              
HinfI         128  GAATC                G^A[ACGT]TC
             1065  GAATC               
MboII         350  TCTTC                GAAGA(8/7)
              353  TCTTC               
MflI          482  GGATCC               [GA]^GATC[CT]
              668  AGATCT              
MluNI         572  TGGCCA               TGG^CCA
              910  TGGCCA              
MscI          572  TGGCCA               TGG^CCA
              910  TGGCCA              
NciI           49  CCGGG                CC^[GC]GG
              867  CCGGG               
PpuMI         524  AGGTCCT              [GA]G^G[AT]CC[CT]
              802  GGGACCC             
SmlI          593  CTCAAG               C^T[CT][GA]AG
             1042  CTCAAG              
StuI          761  AGGCCT               AGG^CCT
              998  AGGCCT              
TfiI          128  GAATC                G^A[AT]TC
             1065  GAATC               
TspRI         914  CAGTG                CA[GC]TG[ACGT][ACGT]^
             1056  CAGTG               
XhoII         482  GGATCC               [GA]^GATC[CT]
              668  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AciI        AluI        AlwI        AspHI       AvaII       
BbvI        BmyI        BpmI        BsaJI       BsiHKAI     
BsiYI       BslI        BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       EaeI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HpaII       
HphI        ItaI        MaeIII      MboI        MnlI        
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       SfaNI       
StyI        TaqI        TseI        Tsp45I      Tsp509I     
TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com