Restriction map for
pF1KB3671
##################################
# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AflII AgeI AhdI AlwI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
BamHI BbeI BbvCI BcgI BciVI
BclI BfrI BglI BglII BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmFI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstXI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DpnII DraII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FokI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeIII MamI
MboI MflI MluI MroI NaeI
NarI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshBI PvuI RcaI
RsrII SacII SalI SapI Sau3AI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AclI 306 AACGTT AA^CGTT
AcsI 811 AAATTT [GA]^AATT[CT]
AflIII 80 ACGTGT A^C[GA][CT]GT
Alw44I 943 GTGCAC G^TGCAC
ApaLI 943 GTGCAC G^TGCAC
ApoI 811 AAATTT [GA]^AATT[CT]
Asp700I 190 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 761 GGACC G^G[AT]CC
AvrII 129 CCTAGG C^CTAGG
BalI 116 TGGCCA TGG^CCA
BanI 976 GGCACC G^G[CT][GA]CC
BanII 712 GAGCTC G[GA]GC[CT]^C
BbrPI 622 CACGTG CAC^GTG
BcnI 445 CCGGG CC^[GC]GG
BfaI 130 CTAG C^TAG
BlnI 129 CCTAGG C^CTAGG
BpmI 649 CTGGAG CTGGAG(16/14)
BsaAI 622 CACGTG [CT]AC^GT[GA]
BsgI 583 GTGCAG GTGCAG(16/14)
BsmI 1097 GCATTC GAATGC(1/-1)
BsrDI 904 GCAATG GCAATG(2/0)
DraIII 76 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 119 CCATGG C^C[GA][CT]GG
EcoNI 659 CCTACGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII 445 CCGG C^CGG
HgaI 994 GCGTC GACGC(5/10)
HpaII 445 CCGG C^CGG
HphI 963 GGTGA GGTGA(8/7)
MaeI 130 CTAG C^TAG
MfeI 455 CAATTG C^AATTG
MluNI 116 TGGCCA TGG^CCA
MscI 116 TGGCCA TGG^CCA
MslI 222 CACTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 445 CCGG C^CGG
MunI 455 CAATTG C^AATTG
NciI 445 CCGGG CC^[GC]GG
NcoI 119 CCATGG C^CATGG
PleI 379 GAGTC GAGTC(4/5)
PmaCI 622 CACGTG CAC^GTG
PmlI 622 CACGTG CAC^GTG
PshAI 922 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 306 AACGTT AA^CGTT
PstI 371 CTGCAG CTGCA^G
PvuII 643 CAGCTG CAG^CTG
SacI 712 GAGCTC GAGCT^C
ScaI 784 AGTACT AGT^ACT
SfcI 371 CTGCAG C^T[GA][CT]AG
TaqI 997 TCGA T^CGA
TthHB8I 997 TCGA T^CGA
XmnI 190 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 637 CGCG CG^CG
639 CGCG
AspHI 712 GAGCTC G[AT]GC[AT]^C
943 GTGCAC
BbsI 190 GAAGAC GAAGAC(2/6)
323 GTCTTC
BmyI 712 GAGCTC G[AGT]GC[ACT]^C
943 GTGCAC
BpuAI 190 GAAGAC GAAGAC(2/6)
323 GTCTTC
BsaI 920 GAGACC GGTCTC(1/5)
405 GGTCTC
BseRI 105 CTCCTC GAGGAG(10/8)
568 GAGGAG
BsiHKAI 712 GAGCTC G[AT]GC[AT]^C
943 GTGCAC
Bsp1286I 712 GAGCTC G[AGT]GC[ACT]^C
943 GTGCAC
BspMI 251 ACCTGC ACCTGC(4/8)
1112 GCAGGT
BsrI 210 CCAGT ACTGG(1/-1)
580 CCAGT
BssHII 636 GCGCGC G^CGCGC
638 GCGCGC
BstUI 637 CGCG CG^CG
639 CGCG
EaeI 116 TGGCCA [CT]^GGCC[GA]
263 CGGCCA
EcoT14I 119 CCATGG C^C[AT][AT]GG
129 CCTAGG
MvnI 637 CGCG CG^CG
639 CGCG
NlaIV 976 GGCACC GG[ACGT]^[ACGT]CC
1036 GGCCCC
StyI 119 CCATGG C^C[AT][AT]GG
129 CCTAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AfaI AluI AlwNI BbvI
BlpI Bpu1102I BsaJI BsiYI BslI
BsmAI BssKI BstNI Cac8I CelII
CfoI Cfr13I DdeI DraI EarI
EcoRII Fnu4HI HaeIII HhaI HinP1I
HinfI ItaI Ksp632I MaeII MboII
MnlI MseI MspA1I MvaI MwoI
NlaIII RsaI Sau96I ScrFI SfaNI
TfiI Tru9I TseI Tsp509I TspRI