Restriction map for pF1KB3604
##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
AspEI       AspI        AvaI        AviII       AvrII       
BalI        BbeI        BbrPI       BcgI        BciVI       
BclI        BfrI        BlnI        BsaAI       BsaHI       
BsaI        BsiEI       BsiWI       BsoBI       Bsp1407I    
BspDI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
Cfr10I      ClaI        CpoI        DraI        DraIII      
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HgaI        
Hin1I       HindIII     HpaI        KasI        KspI        
MfeI        MluI        MluNI       MscI        MunI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SapI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SwaI        Tth111I     
XbaI        XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccI          103  GTCGAC               GT^[AC][GT]AC
AccII         645  CGCG                 CG^CG
AccIII        405  TCCGGA               T^CCGGA
AcsI          112  GAATTT               [GA]^AATT[CT]
ApaI         1016  GGGCCC               GGGCC^C
ApoI          112  GAATTT               [GA]^AATT[CT]
AspHI        1024  GAGCAC               G[AT]GC[AT]^C
BamHI          70  GGATCC               G^GATCC
BbvCI        1177  GCTGAGG              CCTCAGC(-5/-2)
BfaI          158  CTAG                 C^TAG
BglI         1231  GCCGGGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          671  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I      671  GCTTAGC              GC^T[ACGT]AGC
BsaBI         705  GATCGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI         405  TCCGGA               T^CCGGA
BsgI          999  CTGCAC               GTGCAG(16/14)
BsiHKAI      1024  GAGCAC               G[AT]GC[AT]^C
BsmBI         583  GAGACG               CGTCTC(1/5)
BspEI         405  TCCGGA               T^CCGGA
BspMI        1208  ACCTGC               ACCTGC(4/8)
BstUI         645  CGCG                 CG^CG
BstXI         600  CCAGGCCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         671  GCTTAGC              GC^T[ACGT]AGC
DrdI           77  GACACATTAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          860  TGGCCG               [CT]^GGCC[GA]
HaeII          23  AGCGCC               [GA]GCGC^[CT]
MaeI          158  CTAG                 C^TAG
MamI          705  GATCGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          405  TCCGGA               T^CCGGA
MvnI          645  CGCG                 CG^CG
NspI          368  GCATGT               [GA]CATG^[CT]
PflMI          21  CCAGCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         141  GACGGAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         181  CAGCTG               CAG^CTG
SalI          103  GTCGAC               G^TCGAC
SexAI         251  ACCTGGT              A^CC[AT]GGT
SspI         1468  AATATT               AAT^ATT
StuI         1111  AGGCCT               AGG^CCT
Van91I         21  CCAGCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          885  CCAGTCCGAGGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

Acc65I        533  GGTACC               G^GTACC
             1274  GGTACC              
AlwNI          22  CAGCGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              203  CAGAGGCTG           
Asp718I       533  GGTACC               G^GTACC
             1274  GGTACC              
BanII         877  GAGCCC               G[GA]GC[CT]^C
             1016  GGGCCC              
BglII         160  AGATCT               A^GATCT
              296  AGATCT              
BpmI         1264  CTCCAG               CTGGAG(16/14)
              580  CTGGAG              
BsmI          198  GAATGC               GAATGC(1/-1)
              275  GAATGC              
HincII        103  GTCGAC               GT[CT]^[GA]AC
              376  GTCAAC              
HindII        103  GTCGAC               GT[CT]^[GA]AC
              376  GTCAAC              
HinfI         395  GAATC                G^A[ACGT]TC
             1339  GATTC               
KpnI          533  GGTACC               GGTAC^C
             1274  GGTACC              
MslI          474  CATCACCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              522  CATCTTCATG          
MspA1I        181  CAGCTG               C[AC]G^C[GT]G
              801  CCGCTG              
PpuMI        1151  GGGACCC              [GA]G^G[AT]CC[CT]
             1205  AGGACCT             
PstI           33  CTGCAG               CTGCA^G
               92  CTGCAG              
SfaNI         626  GCATC                GCATC(5/9)
              910  GCATC               
TfiI          395  GAATC                G^A[AT]TC
             1339  GATTC               
Tsp45I       1403  GTGAC                ^GT[GC]AC
             1478  GTGAC               
TspRI        1027  CACTG                CA[GC]TG[ACGT][ACGT]^
             1476  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BanI        BbsI        BbvI        BcnI        BmyI        
BpuAI       BsaJI       BsaWI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MspI        
MvaI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       RsaI        Sau3AI      Sau96I      
ScrFI       SfcI        StyI        TaqI        Tru9I       
TseI        Tsp509I     TthHB8I     XhoII       
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