Restriction map for pF1KB3428
##################################
# NO CUT ENZYMES (  154 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbvCI       
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HgaI        Hin1I       HincII      HindII      
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MroI        MunI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tsp509I     Tth111I     TthHB8I     XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AlwNI         502  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaI          164  CCCGAG               C^[CT]CG[GA]G
BanI          489  GGTGCC               G^G[CT][GA]CC
BanII         246  GGGCTC               G[GA]GC[CT]^C
BcgI         1232  CGAAGGACCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          947  CTAG                 C^TAG
BlpI          933  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      933  GCTCAGC              GC^T[ACGT]AGC
BsoBI         164  CCCGAG               C^[CT]CG[GA]G
BsrBI           5  CCGCTC               CCGCTC(-3/-3)
BsrDI         417  CATTGC               GCAATG(2/0)
BstXI         800  CCATGTTTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         933  GCTCAGC              GC^T[ACGT]AGC
CfoI           82  GCGC                 GCG^C
EcoRV         742  GATATC               GAT^ATC
HhaI           82  GCGC                 GCG^C
HinP1I         82  GCGC                 G^CGC
HindIII      1219  AAGCTT               A^AGCTT
MaeI          947  CTAG                 C^TAG
MseI          269  TTAA                 T^TAA
MspA1I        718  CAGCTG               C[AC]G^C[GT]G
PflMI         561  CCAGGCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        1047  GACGGAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          580  CTGCAG               CTGCA^G
PvuII         718  CAGCTG               CAG^CTG
SapI         1185  GCTCTTC              GCTCTTC(1/4)
Tru9I         269  TTAA                 T^TAA
Tsp45I       1066  GTCAC                ^GT[GC]AC
Van91I        561  CCAGGCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccII          11  CGCG                 CG^CG
               83  CGCG                
AspHI         298  GTGCTC               G[AT]GC[AT]^C
             1183  GTGCTC              
AvaII        1053  GGTCC                G^G[AT]CC
             1236  GGACC               
BalI           92  TGGCCA               TGG^CCA
              413  TGGCCA              
BseRI         573  CTCCTC               GAGGAG(10/8)
              463  GAGGAG              
BsiHKAI       298  GTGCTC               G[AT]GC[AT]^C
             1183  GTGCTC              
BsmAI         842  GAGAC                GTCTC(1/5)
             1210  GTCTC               
BstUI          11  CGCG                 CG^CG
               83  CGCG                
BstYI         179  AGATCC               [GA]^GATC[CT]
              656  GGATCT              
DraII        1052  AGGTCCC              [GA]G^G[ACGT]CC[CT]
             1235  AGGACCT             
EcoO109I     1052  AGGTCCC              [GA]G^G[ACGT]CC[CT]
             1235  AGGACCT             
HapII         453  CCGG                 C^CGG
              677  CCGG                
HpaII         453  CCGG                 C^CGG
              677  CCGG                
MaeII         818  ACGT                 A^CGT
             1173  ACGT                
MaeIII        472  GTAAC                ^GT[ACGT]AC
             1066  GTCAC               
MflI          179  AGATCC               [GA]^GATC[CT]
              656  GGATCT              
MluNI          92  TGGCCA               TGG^CCA
              413  TGGCCA              
MscI           92  TGGCCA               TGG^CCA
              413  TGGCCA              
MslI          906  CATTGATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1035  CACCATTATG          
MspI          453  CCGG                 C^CGG
              677  CCGG                
MvnI           11  CGCG                 CG^CG
               83  CGCG                
PpuMI        1052  AGGTCCC              [GA]G^G[AT]CC[CT]
             1235  AGGACCT             
SfcI          580  CTGCAG               C^T[GA][CT]AG
              715  CTACAG              
XcmI           68  CCAACAGCGAAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              435  CCAGGGCTTGTGTGG     
XhoII         179  AGATCC               [GA]^GATC[CT]
              656  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BbsI        
BbvI        BmyI        BpmI        BpuAI       BsaJI       
BsgI        BsiYI       BslI        BsmFI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       EaeI        
EarI        EcoRII      Fnu4HI      FokI        HaeIII      
HinfI       HphI        ItaI        Ksp632I     MboI        
MboII       MnlI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       TseI        TspRI       
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