Restriction map for pF1KB3393
##################################
# NO CUT ENZYMES (  133 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AocI        
Aor51HI     ApoI        AscI        AseI        AsnI        
AspI        AvaI        AvrII       BamHI       BbeI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsiEI       BsiWI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KspI        MaeI        MfeI        MluI        
MroI        MseI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmeI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        Tru9I       Tsp509I     
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

Acc65I        128  GGTACC               G^GTACC
AlwNI         196  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp718I       128  GGTACC               G^GTACC
BalI          339  TGGCCA               TGG^CCA
BbrPI         708  CACGTG               CAC^GTG
BbsI          268  GTCTTC               GAAGAC(2/6)
BglI          611  GCCAGCTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          571  GCTCAGC              GC^T[ACGT]AGC
BpmI          427  CTGGAG               CTGGAG(16/14)
Bpu1102I      571  GCTCAGC              GC^T[ACGT]AGC
BpuAI         268  GTCTTC               GAAGAC(2/6)
BsaAI         708  CACGTG               [CT]AC^GT[GA]
BsaBI         831  GATGACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI          492  CTGCAC               GTGCAG(16/14)
BsmBI         430  GAGACG               CGTCTC(1/5)
BspHI         779  TCATGA               T^CATGA
BsrDI          84  CATTGC               GCAATG(2/0)
BstAPI        195  GCAGAAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         629  CCATCCACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         680  AGATCC               [GA]^GATC[CT]
CelII         571  GCTCAGC              GC^T[ACGT]AGC
HinfI          21  GATTC                G^A[ACGT]TC
KpnI          128  GGTACC               GGTAC^C
MamI          831  GATGACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          680  AGATCC               [GA]^GATC[CT]
MluNI         339  TGGCCA               TGG^CCA
MscI          339  TGGCCA               TGG^CCA
NcoI          456  CCATGG               C^CATGG
NspI          899  GCATGC               [GA]CATG^[CT]
PmaCI         708  CACGTG               CAC^GTG
PmlI          708  CACGTG               CAC^GTG
PstI          193  CTGCAG               CTGCA^G
PvuII         255  CAGCTG               CAG^CTG
RcaI          779  TCATGA               T^CATGA
SacI          569  GAGCTC               GAGCT^C
SfaNI         392  GCATC                GCATC(5/9)
SfcI          193  CTGCAG               C^T[GA][CT]AG
SphI          899  GCATGC               GCATG^C
TfiI           21  GATTC                G^A[AT]TC
XhoII         680  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AhdI          352  GACTTTACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              783  GACGCAGTGTC         
Alw44I        379  GTGCAC               G^TGCAC
              442  GTGCAC              
AlwI          681  GATCC                GGATC(4/5)
              764  GGATC               
ApaI          245  GGGCCC               GGGCC^C
              289  GGGCCC              
ApaLI         379  GTGCAC               G^TGCAC
              442  GTGCAC              
AspEI         352  GACTTTACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              783  GACGCAGTGTC         
AviII         473  TGCGCA               TGC^GCA
              896  TGCGCA              
BanI          128  GGTACC               G^G[CT][GA]CC
              211  GGCACC              
BbvI          860  GCAGC                GCAGC(8/12)
              201  GCTGC               
BcnI          234  CCGGG                CC^[GC]GG
              879  CCGGG               
BsmAI         430  GAGAC                GTCTC(1/5)
              791  GTCTC               
DdeI          110  CTAAG                C^T[ACGT]AG
              572  CTCAG               
EaeI          218  CGGCCA               [CT]^GGCC[GA]
              339  TGGCCA              
Eam1105I      352  GACTTTACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              783  GACGCAGTGTC         
FspI          473  TGCGCA               TGC^GCA
              896  TGCGCA              
HaeII         419  AGCGCC               [GA]GCGC^[CT]
              588  GGCGCT              
HapII         234  CCGG                 C^CGG
              879  CCGG                
HpaII         234  CCGG                 C^CGG
              879  CCGG                
MaeII         358  ACGT                 A^CGT
              709  ACGT                
MaeIII        262  GTGAC                ^GT[ACGT]AC
              398  GTCAC               
MspA1I        255  CAGCTG               C[AC]G^C[GT]G
              659  CCGCTG              
MspI          234  CCGG                 C^CGG
              879  CCGG                
NciI          234  CCGGG                CC^[GC]GG
              879  CCGGG               
TseI          201  GCTGC                G^C[AT]GC
              860  GCAGC               
Tsp45I        262  GTGAC                ^GT[GC]AC
              398  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        Asp700I     
AspHI       AvaII       BanII       BmyI        BsaJI       
BsiHKAI     BsiYI       BslI        BsmFI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DpnII       
DraII       DsaI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HgaI        HhaI        HinP1I      HphI        ItaI        
Ksp632I     MboI        MboII       MnlI        MslI        
MvaI        MvnI        MwoI        NdeII       NlaIII      
NlaIV       PpuMI       RsaI        SapI        Sau3AI      
Sau96I      ScrFI       StyI        TspRI       XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com