Restriction map for pF1KB3331
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BcnI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaHI       
BsaI        BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsoBI       BspEI       BspHI       BsrBI       
BsrDI       BsrFI       BssHII      BssSI       BstAPI      
BstBI       BstUI       Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      CpoI        DraI        DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoRV       
FseI        FspI        HaeII       HaeIII      HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        HphI        KasI        KpnI        KspI        
MaeII       MluI        MluNI       MroI        MscI        
MvnI        NaeI        NarI        NciI        NcoI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    StuI        
SwaI        Tth111I     XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI         1462  GTATAC               GT^[AC][GT]AC
AflIII         68  ACATGT               A^C[GA][CT]GT
AlwNI         305  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
BanII         771  GAGCCC               G[GA]GC[CT]^C
BbsI          796  GAAGAC               GAAGAC(2/6)
BclI         1410  TGATCA               T^GATCA
BglII         686  AGATCT               A^GATCT
BmyI          771  GAGCCC               G[AGT]GC[ACT]^C
BpuAI         796  GAAGAC               GAAGAC(2/6)
BsaBI         409  GATGCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1228  TCCGGT               [AT]^CCGG[AT]
BseRI           7  GAGGAG               GAGGAG(10/8)
BsiYI         304  CCAGGACCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          304  CCAGGACCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         502  GAGAC                GTCTC(1/5)
Bsp1286I      771  GAGCCC               G[AGT]GC[ACT]^C
BspDI         898  ATCGAT               AT^CGAT
BspLU11I       68  ACATGT               A^CATGT
BspMI         283  GCAGGT               ACCTGC(4/8)
Bst1107I     1462  GTATAC               GTA^TAC
BstEII        909  GGTAACC              G^GT[ACGT]ACC
BstPI         909  GGTAACC              G^GT[ACGT]ACC
BstXI        1501  CCAGTCAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1462  GTATAC               GTA^TAC
ClaI          898  ATCGAT               AT^CGAT
DsaI          577  CCGTGG               C^C[GA][CT]GG
EcoO65I       909  GGTAACC              G^GT[ACGT]ACC
EcoRI         486  GAATTC               G^AATTC
EcoT14I       401  CCAAGG               C^C[AT][AT]GG
EcoT22I      1306  ATGCAT               ATGCA^T
FauI          747  GCGGG                CCCGC(4/6)
FbaI         1410  TGATCA               T^GATCA
HapII        1229  CCGG                 C^CGG
HindIII       139  AAGCTT               A^AGCTT
HpaII        1229  CCGG                 C^CGG
MamI          409  GATGCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          491  CAATTG               C^AATTG
MspI         1229  CCGG                 C^CGG
MunI          491  CAATTG               C^AATTG
NsiI         1306  ATGCAT               ATGCA^T
NspI           68  ACATGT               [GA]CATG^[CT]
PflMI         304  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          114  GAGTC                GAGTC(4/5)
PstI         1019  CTGCAG               CTGCA^G
ScaI          739  AGTACT               AGT^ACT
SspI         1392  AATATT               AAT^ATT
StyI          401  CCAAGG               C^C[AT][AT]GG
Van91I        304  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          556  CCAAAACCCCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AciI          379  GCGG                 CCGC(-3/-1)
              747  GCGG                
BanI          569  GGCACC               G^G[CT][GA]CC
             1300  GGCACC              
BfaI          428  CTAG                 C^TAG
              701  CTAG                
BsmFI         692  GGGAC                GGGAC(10/14)
             1316  GGGAC               
BsmI          213  GAATGC               GAATGC(1/-1)
              347  GAATGC              
Bsp1407I       27  TGTACA               T^GTACA
              966  TGTACA              
BsrGI          27  TGTACA               T^GTACA
              966  TGTACA              
EarI           54  GAAGAG               CTCTTC(1/4)
              109  GAAGAG              
Ksp632I        54  GAAGAG               CTCTTC(1/4)
              109  GAAGAG              
MaeI          428  CTAG                 C^TAG
              701  CTAG                
NdeI          169  CATATG               CA^TATG
              227  CATATG              
SspBI          27  TGTACA               T^GTACA
              966  TGTACA              
Tsp45I        116  GTCAC                ^GT[GC]AC
             1096  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
AvaII       BbvI        BpmI        BsaJI       BsgI        
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cfr13I      DdeI        DpnII       DraII       EcoO109I    
EcoRII      Fnu4HI      FokI        HinfI       ItaI        
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MvaI        MwoI        
NdeII       NlaIII      NlaIV       PpuMI       PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TaqI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     XhoII       
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