Restriction map for pF1KB3313
##################################
# NO CUT ENZYMES (  105 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AgeI        Alw44I      Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AspHI       AvaI        
AviII       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BsaWI       BseAI       BsgI        BsiHKAI     
BsiWI       BsmI        BsoBI       BspDI       BspEI       
BspHI       BsrBI       BsrFI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Cfr10I      ClaI        Eco47III    EcoO65I     EcoRI       
EcoT22I     FbaI        FseI        FspI        HindIII     
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MroI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        SacI        SacII       
SalI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AatII         294  GACGTC               GACGT^C
AcsI          133  AAATTT               [GA]^AATT[CT]
AcyI          294  GACGTC               G[GA]^CG[CT]C
AflIII       1076  ACATGT               A^C[GA][CT]GT
AhdI          289  GACCAGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          267  CCTCAGG              CC^T[ACGT]AGG
ApoI          133  AAATTT               [GA]^AATT[CT]
Asp700I       117  GAAATGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         289  GACCAGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1096  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        1278  CCTAGG               C^CTAGG
BlnI         1278  CCTAGG               C^CTAGG
BpmI         1211  CTGGAG               CTGGAG(16/14)
BsaAI        1069  TACGTG               [CT]AC^GT[GA]
BsaHI         294  GACGTC               G[GA]^CG[CT]C
BsaI          826  GGTCTC               GGTCTC(1/5)
BseRI         343  GAGGAG               GAGGAG(10/8)
BsiEI          48  CGGCCG               CG[GA][CT]^CG
Bsp1407I     1589  TGTACA               T^GTACA
BspLU11I     1076  ACATGT               A^CATGT
BspMI         967  ACCTGC               ACCTGC(4/8)
BsrGI        1589  TGTACA               T^GTACA
BstAPI       1238  GCAGCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I        267  CCTCAGG              CC^T[ACGT]AGG
CpoI          582  CGGACCG              CG^G[AT]CCG
DraI          598  TTTAAA               TTT^AAA
DraIII        871  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1380  GACAGGCCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         1565  CCATGG               C^C[GA][CT]GG
EagI           48  CGGCCG               C^GGCCG
Eam1105I      289  GACCAGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI          48  CGGCCG               C^GGCCG
Eco52I         48  CGGCCG               C^GGCCG
Eco81I        267  CCTCAGG              CC^T[ACGT]AGG
EcoRV          53  GATATC               GAT^ATC
HgaI         1090  GACGC                GACGC(5/10)
Hin1I         294  GACGTC               G[GA]^CG[CT]C
MslI          768  CACAGCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI         1565  CCATGG               C^CATGG
NspI         1076  ACATGT               [GA]CATG^[CT]
PflFI        1096  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1251  CCAGGCGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1013  AGGACCT              [GA]G^G[AT]CC[CT]
RsrII         582  CGGACCG              CG^G[AT]CCG
SapI          449  GCTCTTC              GCTCTTC(1/4)
SexAI        1016  ACCTGGT              A^CC[AT]GGT
SspBI        1589  TGTACA               T^GTACA
Tth111I      1096  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1251  CCAGGCGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1433  CCAGCATCTTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          117  GAAATGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccII         109  CGCG                 CG^CG
              594  CGCG                
ApaI          929  GGGCCC               GGGCC^C
             1026  GGGCCC              
BamHI         996  GGATCC               G^GATCC
             1049  GGATCC              
BbsI         1263  GAAGAC               GAAGAC(2/6)
              441  GTCTTC              
BcnI          503  CCCGG                CC^[GC]GG
              549  CCCGG               
BfaI          557  CTAG                 C^TAG
             1279  CTAG                
BlpI          663  GCTAAGC              GC^T[ACGT]AGC
             1523  GCTTAGC             
Bpu1102I      663  GCTAAGC              GC^T[ACGT]AGC
             1523  GCTTAGC             
BpuAI        1263  GAAGAC               GAAGAC(2/6)
              441  GTCTTC              
BsaBI         634  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1308  GATGCTGATC          
BsrDI        1530  CATTGC               GCAATG(2/0)
             1292  GCAATG              
BstUI         109  CGCG                 CG^CG
              594  CGCG                
CelII         663  GCTAAGC              GC^T[ACGT]AGC
             1523  GCTTAGC             
FauI          949  CCCGC                CCCGC(4/6)
              510  GCGGG               
HaeII        1139  GGCGCT               [GA]GCGC^[CT]
             1605  GGCGCT              
HincII       1080  GTCAAC               GT[CT]^[GA]AC
             1549  GTCAAC              
HindII       1080  GTCAAC               GT[CT]^[GA]AC
             1549  GTCAAC              
MaeI          557  CTAG                 C^TAG
             1279  CTAG                
MamI          634  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1308  GATGCTGATC          
MvnI          109  CGCG                 CG^CG
              594  CGCG                
NciI          503  CCCGG                CC^[GC]GG
              549  CCCGG               
TaqI          257  TCGA                 T^CGA
              327  TCGA                
TthHB8I       257  TCGA                 T^CGA
              327  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AvaII       BalI        BanII       BbvI        BmyI        
BsaJI       BsiYI       BslI        BsmAI       BsmBI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EaeI        
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MspA1I      MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       XhoII       
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