Restriction map for pF1KB3313
 (Linear) (Six-base) MAPSORT of: KIBB3313.seq  Check: 166  from: 1  to: 1659



KIBB3313.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AatII G_ACGT'C

Cuts at:      0     298    1659 
   Size:        298    1361
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     199     416     428     437     974    1659 
   Size:        199     217      12       9     537     685

  Fragments arranged by size: 

                 685     537     217     199      12       9
 
AflIII A'CryG_T

Cuts at:      0    1076    1659 
   Size:       1076     583
 
AhdI GACnn_n'nnGTC

Cuts at:      0     294    1659 
   Size:        294    1365
 
AleI CACnn'nnGTG

Cuts at:      0     772    1659 
   Size:        772     887
 
AlwNI CAG_nnn'CTG

Cuts at:      0     880    1176    1244    1659 
   Size:        880     296      68     415

  Fragments arranged by size: 

                 880     415     296      68
 
ApaI G_GGCC'C

Cuts at:      0     933    1030    1659 
   Size:        933      97     629

  Fragments arranged by size: 

                 933     629      97
 
ApoI r'AATT_y

Cuts at:      0     133    1659 
   Size:        133    1526
 
AvrII C'CTAG_G

Cuts at:      0    1278    1659 
   Size:       1278     381
 
BamHI G'GATC_C

Cuts at:      0     996    1049    1659 
   Size:        996      53     610

  Fragments arranged by size: 

                 996     610      53
 
BanII G_rGCy'C

Cuts at:      0     489     933    1030    1659 
   Size:        489     444      97     629

  Fragments arranged by size: 

                 629     489     444      97
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     434    1270    1659 
   Size:        434     836     389

  Fragments arranged by size: 

                 836     434     389
 
BlpI GC'TnA_GC

Cuts at:      0     664    1524    1659 
   Size:        664     860     135

  Fragments arranged by size: 

                 860     664     135
 
Bme1580I G_kGCm'C

Cuts at:      0     933    1030    1659 
   Size:        933      97     629

  Fragments arranged by size: 

                 933     629      97
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1025    1659 
   Size:       1025     634
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     480    1659 
   Size:        480    1179
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1232    1659 
   Size:       1232     427
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     832    1659 
   Size:        832     827
 
BsaAI yAC'GTr

Cuts at:      0    1071    1659 
   Size:       1071     588
 
BsaBI GATnn'nnATC

Cuts at:      0     638    1312    1659 
   Size:        638     674     347

  Fragments arranged by size: 

                 674     638     347
 
BsaHI Gr'CG_yC

Cuts at:      0     295    1659 
   Size:        295    1364
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     358    1659 
   Size:        358    1301
 
BseYI C'CCAG_C

Cuts at:      0    1023    1391    1536    1659 
   Size:       1023     368     145     123

  Fragments arranged by size: 

                1023     368     145     123
 
BsiEI CG_ry'CG

Cuts at:      0      51    1659 
   Size:         51    1608
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     852     966    1164    1659 
   Size:        852     114     198     495

  Fragments arranged by size: 

                 852     495     198     114
 
Bsp1286I G_dGCh'C

Cuts at:      0     489     933    1030    1659 
   Size:        489     444      97     629

  Fragments arranged by size: 

                 629     489     444      97
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     976    1659 
   Size:        976     683
 
BsrDI GCAATG_nn'

Cuts at:      0    1299    1529    1659 
   Size:       1299     230     130

  Fragments arranged by size: 

                1299     230     130
 
BsrGI T'GTAC_A

Cuts at:      0    1589    1659 
   Size:       1589      70
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1244    1659 
   Size:       1244     415
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1017    1536    1608    1659 
   Size:       1017     519      72      51

  Fragments arranged by size: 

                1017     519      72      51
 
BstYI r'GATC_y

Cuts at:      0     537     639     996    1049    1515    1659 
   Size:        537     102     357      53     466     144

  Fragments arranged by size: 

                 537     466     357     144     102      53
 
Bsu36I CC'TnA_GG

Cuts at:      0     268    1659 
   Size:        268    1391
 
BtgI C'CryG_G

Cuts at:      0    1565    1659 
   Size:       1565      94
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1270    1659 
   Size:       1270     389
 
BtsI GCAGTG_nn'

Cuts at:      0     893    1659 
   Size:        893     766
 
DraI TTT'AAA

Cuts at:      0     600    1659 
   Size:        600    1059
 
DraIII CAC_nnn'GTG

Cuts at:      0     876    1659 
   Size:        876     783
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1386    1659 
   Size:       1386     273
 
EaeI y'GGCC_r

Cuts at:      0      48      64     578     590    1007    1343    1430    1659 
   Size:         48      16     514      12     417     336      87     229

  Fragments arranged by size: 

                 514     417     336     229      87      48      16      12
            
 
EagI C'GGCC_G

Cuts at:      0      48    1659 
   Size:         48    1611
 
EarI CTCTTCn'nnn_

Cuts at:      0     254     427     456     722    1659 
   Size:        254     173      29     266     937

  Fragments arranged by size: 

                 937     266     254     173      29
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1042    1623    1659 
   Size:       1042     581      36

  Fragments arranged by size: 

                1042     581      36
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     199     416     428     437     974    1232    1659 
   Size:        199     217      12       9     537     258     427

  Fragments arranged by size: 

                 537     427     258     217     199      12       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      65    1372    1417    1659 
   Size:         65    1307      45     242

  Fragments arranged by size: 

                1307     242      65      45
 
EcoO109I rG'GnC_Cy

Cuts at:      0     929    1014    1027    1659 
   Size:        929      85      13     632

  Fragments arranged by size: 

                 929     632      85      13
 
EcoRV GAT'ATC

Cuts at:      0      55    1659 
   Size:         55    1604
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     138    1287    1659 
   Size:        138    1149     372

  Fragments arranged by size: 

                1149     372     138
 
HaeII r_GCGC'y

Cuts at:      0    1143    1609    1659 
   Size:       1143     466      50

  Fragments arranged by size: 

                1143     466      50
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      66      98     280     312    1659 
   Size:         66      32     182      32    1347

  Fragments arranged by size: 

                1347     182      66      32      32
 
HincII GTy'rAC

Cuts at:      0    1082    1551    1659 
   Size:       1082     469     108

  Fragments arranged by size: 

                1082     469     108
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     875     974    1582    1659 
   Size:        875      99     608      77

  Fragments arranged by size: 

                 875     608      99      77
 
MscI TGG'CCA

Cuts at:      0      66    1345    1432    1659 
   Size:         66    1279      87     227

  Fragments arranged by size: 

                1279     227      87      66
 
MslI CAynn'nnrTG

Cuts at:      0     772    1659 
   Size:        772     887
 
MspA1I CmG'CkG

Cuts at:      0     177     685     760     952    1176    1244    1573    1659 
   Size:        177     508      75     192     224      68     329      86

  Fragments arranged by size: 

                 508     329     224     192     177      86      75      68
            
 
NcoI C'CATG_G

Cuts at:      0    1565    1659 
   Size:       1565      94
 
NspI r_CATG'y

Cuts at:      0    1080    1659 
   Size:       1080     579
 
PasI CC'CwG_GG

Cuts at:      0    1057    1659 
   Size:       1057     602
 
PciI A'CATG_T

Cuts at:      0    1076    1659 
   Size:       1076     583
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1257    1659 
   Size:       1257     402
 
PfoI T'CCnGG_A

Cuts at:      0     548     920    1442    1659 
   Size:        548     372     522     217

  Fragments arranged by size: 

                 548     522     372     217
 
PpuMI rG'GwC_Cy

Cuts at:      0    1014    1659 
   Size:       1014     645
 
PspOMI G'GGCC_C

Cuts at:      0     929    1026    1659 
   Size:        929      97     633

  Fragments arranged by size: 

                 929     633      97
 
PstI C_TGCA'G

Cuts at:      0     900    1240    1578    1659 
   Size:        900     340     338      81

  Fragments arranged by size: 

                 900     340     338      81
 
PvuII CAG'CTG

Cuts at:      0     177     685     760    1176    1244    1573    1659 
   Size:        177     508      75     416      68     329      86

  Fragments arranged by size: 

                 508     416     329     177      86      75      68
 
RsrII CG'GwC_CG

Cuts at:      0     583    1659 
   Size:        583    1076
 
SapI GCTCTTCn'nnn_

Cuts at:      0     456    1659 
   Size:        456    1203
 
SexAI A'CCwGG_T

Cuts at:      0    1016    1659 
   Size:       1016     643
 
SfcI C'TryA_G

Cuts at:      0     896    1236    1574    1659 
   Size:        896     340     338      85

  Fragments arranged by size: 

                 896     340     338      85
 
StyI C'CwwG_G

Cuts at:      0      61     822    1001    1010    1278    1329    1565    1601 
   Size:         61     761     179       9     268      51     236      36

Cuts at:   1601    1659 
   Size:         58

  Fragments arranged by size: 

                 761     268     236     179      61      58      51      36
                   9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     600     807    1528    1659 
   Size:        600     207     721     131

  Fragments arranged by size: 

                 721     600     207     131
 
TatI w'GTAC_w

Cuts at:      0    1589    1659 
   Size:       1589      70
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1438    1470    1659 
   Size:       1438      32     189

  Fragments arranged by size: 

                1438     189      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1099    1659 
   Size:       1099     560
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1440    1659 
   Size:       1440     219
 
XmnI GAAnn'nnTTC

Cuts at:      0     121    1659 
   Size:        121    1538
 
ZraI GAC'GTC

Cuts at:      0     296    1659 
   Size:        296    1363
 

 Enzymes that do cut:

    AatII     AcuI   AflIII     AhdI     AleI    AlwNI     ApaI     ApoI
    AvrII    BamHI    BanII     BbsI     BlpI Bme1580I     BmrI     BplI
     BpmI     BsaI    BsaAI    BsaBI    BsaHI    BseRI    BseYI    BsiEI
    BsmBI Bsp1286I    BspMI    BsrDI    BsrGI   BstAPI    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI
    HaeII    Hin4I   HincII     MmeI     MscI     MslI   MspA1I     NcoI
     NspI     PasI     PciI    PflMI     PfoI    PpuMI   PspOMI     PstI
    PvuII    RsrII     SapI    SexAI     SfcI     StyI    TaqII     TatI
     TstI  Tth111I     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AlfI
     AloI    ApaLI     AscI     AseI    AsiSI     AvaI     BaeI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
    BmgBI     BmtI   Bpu10I    BpuEI    BsaWI    BsaXI     BsgI  BsiHKAI
    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrFI   BssHII    BssSI
    BstBI   BstEII  BstZ17I     ClaI    CspCI  EcoICRI    EcoRI     FseI
     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI     SacI    SacII     SalI    SanDI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     XbaI     XhoI     XmaI
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