Restriction map for pF1KB3292
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AciI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AspEI       AspI        AvaI        AviII       AvrII       
BamHI       BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BcnI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmBI       BsoBI       
BspEI       BspMI       BsrBI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      CpoI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRV       EcoT22I     FauI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HpaII       KasI        KspI        
MamI        MfeI        MluI        MroI        MspA1I      
MspI        MunI        MvnI        MwoI        NaeI        
NarI        NciI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PvuI        PvuII       RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SwaI        
Tth111I     XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I       1238  GGTACC               G^GTACC
AclI          554  AACGTT               AA^CGTT
AseI          418  ATTAAT               AT^TAAT
AsnI          418  ATTAAT               AT^TAAT
Asp718I      1238  GGTACC               G^GTACC
AspHI         387  GAGCAC               G[AT]GC[AT]^C
BalI          668  TGGCCA               TGG^CCA
BglII         286  AGATCT               A^GATCT
BseRI         950  GAGGAG               GAGGAG(10/8)
BsiHKAI       387  GAGCAC               G[AT]GC[AT]^C
BsmI           88  GAATGC               GAATGC(1/-1)
Bsp1407I      640  TGTACA               T^GTACA
BspDI         712  ATCGAT               AT^CGAT
BspHI         162  TCATGA               T^CATGA
BsrDI        1619  GCAATG               GCAATG(2/0)
BsrGI         640  TGTACA               T^GTACA
BstYI         286  AGATCT               [GA]^GATC[CT]
Cac8I         748  GCAGGC               GC[ACGT]^[ACGT]GC
ClaI          712  ATCGAT               AT^CGAT
DdeI         1204  CTAAG                C^T[ACGT]AG
DraI          235  TTTAAA               TTT^AAA
DraII         168  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EaeI          668  TGGCCA               [CT]^GGCC[GA]
EcoNI         160  CCTCATGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      168  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRI        1126  GAATTC               G^AATTC
KpnI         1238  GGTACC               GGTAC^C
MflI          286  AGATCT               [GA]^GATC[CT]
MluNI         668  TGGCCA               TGG^CCA
MscI          668  TGGCCA               TGG^CCA
MslI          711  CATCGATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI          923  CATATG               CA^TATG
PflMI        1377  CCATGGTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          846  GACTC                GAGTC(4/5)
PpuMI         168  AGGTCCT              [GA]G^G[AT]CC[CT]
PshBI         418  ATTAAT               AT^TAAT
Psp1406I      554  AACGTT               AA^CGTT
PstI         1543  CTGCAG               CTGCA^G
RcaI          162  TCATGA               T^CATGA
SfcI         1543  CTGCAG               C^T[GA][CT]AG
SspBI         640  TGTACA               T^GTACA
SspI         1671  AATATT               AAT^ATT
Van91I       1377  CCATGGTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         286  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AlwI          210  GGATC                GGATC(4/5)
              674  GGATC               
AlwNI         749  CAGGCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1564  CAGTGCCTG           
Asp700I       448  GAAATTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1134  GAATTTCTTC          
BanI         1238  GGTACC               G^G[CT][GA]CC
             1599  GGTGCC              
BciVI         330  GTATCC               GTATCC(6/5)
              801  GTATCC              
BmyI          387  GAGCAC               G[AGT]GC[ACT]^C
             1600  GTGCCC              
BsaJI         869  CCATGG               C^C[ACGT][ACGT]GG
             1377  CCATGG              
BsmAI         479  GAGAC                GTCTC(1/5)
             1613  GAGAC               
BsmFI         704  GTCCC                GGGAC(10/14)
             1632  GTCCC               
Bsp1286I      387  GAGCAC               G[AGT]GC[ACT]^C
             1600  GTGCCC              
BspLU11I     1020  ACATGT               A^CATGT
             1029  ACATGT              
BstBI         542  TTCGAA               TT^CGAA
             1649  TTCGAA              
DsaI          869  CCATGG               C^C[GA][CT]GG
             1377  CCATGG              
EarI          848  CTCTTC               CTCTTC(1/4)
             1585  GAAGAG              
EcoT14I       869  CCATGG               C^C[AT][AT]GG
             1377  CCATGG              
Ksp632I       848  CTCTTC               CTCTTC(1/4)
             1585  GAAGAG              
NcoI          869  CCATGG               C^CATGG
             1377  CCATGG              
NspV          542  TTCGAA               TT^CGAA
             1649  TTCGAA              
SfuI          542  TTCGAA               TT^CGAA
             1649  TTCGAA              
StuI          493  AGGCCT               AGG^CCT
              879  AGGCCT              
StyI          869  CCATGG               C^C[AT][AT]GG
             1377  CCATGG              
TfiI         1261  GATTC                G^A[AT]TC
             1317  GAATC               
Tsp45I       1277  GTCAC                ^GT[GC]AC
             1657  GTCAC               
XbaI          141  TCTAGA               T^CTAGA
             1326  TCTAGA              
XmnI          448  GAAATTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1134  GAATTTCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AcsI        AfaI        AflIII      AluI        ApoI        
AvaII       BbvI        BclI        BfaI        BpmI        
BsiYI       BslI        BsrI        BssKI       BstF5I      
BstNI       Cfr13I      DpnII       EcoRII      FbaI        
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        MaeI        MaeII       MaeIII      MboI        
MboII       MnlI        MseI        MvaI        NdeII       
NlaIII      NlaIV       NspI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       TaqI        Tru9I       
TseI        Tsp509I     TspRI       TthHB8I     
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