Restriction map for pF1KB3292
 (Linear) (Six-base) MAPSORT of: KIBB3292.seq  Check: 5634  from: 1  to: 1677



KIBB3292.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

Acc65I G'GTAC_C

Cuts at:      0    1238    1677 
   Size:       1238     439
 
AclI AA'CG_TT

Cuts at:      0     555    1677 
   Size:        555    1122
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      66    1677 
   Size:         66    1611
 
AflIII A'CryG_T

Cuts at:      0    1020    1029    1055    1677 
   Size:       1020       9      26     622

  Fragments arranged by size: 

                1020     622      26       9
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     412    1677 
   Size:        412    1265
 
AlwNI CAG_nnn'CTG

Cuts at:      0     754    1569    1677 
   Size:        754     815     108

  Fragments arranged by size: 

                 815     754     108
 
ApoI r'AATT_y

Cuts at:      0       7     965    1126    1134    1268    1364    1677 
   Size:          7     958     161       8     134      96     313

  Fragments arranged by size: 

                 958     313     161     134      96       8       7
 
AseI AT'TA_AT

Cuts at:      0     419    1677 
   Size:        419    1258
 
BanI G'GyrC_C

Cuts at:      0    1238    1599    1677 
   Size:       1238     361      78

  Fragments arranged by size: 

                1238     361      78
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     341     812    1677 
   Size:        341     471     865

  Fragments arranged by size: 

                 865     471     341
 
BclI T'GATC_A

Cuts at:      0     566     611     719    1677 
   Size:        566      45     108     958

  Fragments arranged by size: 

                 958     566     108      45
 
BglII A'GATC_T

Cuts at:      0     286    1677 
   Size:        286    1391
 
Bme1580I G_kGCm'C

Cuts at:      0    1604    1677 
   Size:       1604      73
 
BmgBI CAC'GTC

Cuts at:      0    1276    1677 
   Size:       1276     401
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1465    1522    1677 
   Size:       1465      57     155

  Fragments arranged by size: 

                1465     155      57
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     152     194     676    1139    1677 
   Size:        152      42     482     463     538

  Fragments arranged by size: 

                 538     482     463     152      42
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     578     608    1677 
   Size:        578      30    1069

  Fragments arranged by size: 

                1069     578      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     965    1677 
   Size:        965     712
 
BseYI C'CCAG_C

Cuts at:      0    1603    1634    1677 
   Size:       1603      31      43

  Fragments arranged by size: 

                1603      43      31
 
BsiHKAI G_wGCw'C

Cuts at:      0     391    1677 
   Size:        391    1286
 
BsmI GAATG_Cn'

Cuts at:      0      94    1677 
   Size:         94    1583
 
Bsp1286I G_dGCh'C

Cuts at:      0     391    1604    1677 
   Size:        391    1213      73

  Fragments arranged by size: 

                1213     391      73
 
BspHI T'CATG_A

Cuts at:      0     162    1677 
   Size:        162    1515
 
BsrDI GCAATG_nn'

Cuts at:      0    1626    1677 
   Size:       1626      51
 
BsrGI T'GTAC_A

Cuts at:      0     640    1677 
   Size:        640    1037
 
BstBI TT'CG_AA

Cuts at:      0     543    1650    1677 
   Size:        543    1107      27

  Fragments arranged by size: 

                1107     543      27
 
BstYI r'GATC_y

Cuts at:      0     286    1677 
   Size:        286    1391
 
BtgI C'CryG_G

Cuts at:      0     869    1377    1677 
   Size:        869     508     300

  Fragments arranged by size: 

                 869     508     300
 
BtsI GCAGTG_nn'

Cuts at:      0     991    1645    1677 
   Size:        991     654      32

  Fragments arranged by size: 

                 991     654      32
 
ClaI AT'CG_AT

Cuts at:      0     713    1677 
   Size:        713     964
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     338     373    1677 
   Size:        338      35    1304

  Fragments arranged by size: 

                1304     338      35
 
DraI TTT'AAA

Cuts at:      0     237    1677 
   Size:        237    1440
 
EaeI y'GGCC_r

Cuts at:      0     668    1677 
   Size:        668    1009
 
EarI CTCTTCn'nnn_

Cuts at:      0     854    1580    1677 
   Size:        854     726      97

  Fragments arranged by size: 

                 854     726      97
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      66     152     194     676    1139    1677 
   Size:         66      86      42     482     463     538

  Fragments arranged by size: 

                 538     482     463      86      66      42
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     164    1677 
   Size:        164    1513
 
EcoO109I rG'GnC_Cy

Cuts at:      0     169    1677 
   Size:        169    1508
 
EcoRI G'AATT_C

Cuts at:      0    1126    1677 
   Size:       1126     551
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1155    1677 
   Size:       1155     522
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1174    1206    1677 
   Size:       1174      32     471

  Fragments arranged by size: 

                1174     471      32
 
KpnI G_GTAC'C

Cuts at:      0    1242    1677 
   Size:       1242     435
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1005    1341    1677 
   Size:       1005     336     336

  Fragments arranged by size: 

                1005     336     336
 
MscI TGG'CCA

Cuts at:      0     670    1677 
   Size:        670    1007
 
MslI CAynn'nnrTG

Cuts at:      0     715    1677 
   Size:        715     962
 
NcoI C'CATG_G

Cuts at:      0     869    1377    1677 
   Size:        869     508     300

  Fragments arranged by size: 

                 869     508     300
 
NdeI CA'TA_TG

Cuts at:      0     924    1677 
   Size:        924     753
 
NspI r_CATG'y

Cuts at:      0    1016    1024    1033    1552    1677 
   Size:       1016       8       9     519     125

  Fragments arranged by size: 

                1016     519     125       9       8
 
PciI A'CATG_T

Cuts at:      0    1020    1029    1677 
   Size:       1020       9     648

  Fragments arranged by size: 

                1020     648       9
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1383    1677 
   Size:       1383     294
 
PfoI T'CCnGG_A

Cuts at:      0     171     620    1062    1677 
   Size:        171     449     442     615

  Fragments arranged by size: 

                 615     449     442     171
 
PpuMI rG'GwC_Cy

Cuts at:      0     169    1677 
   Size:        169    1508
 
PstI C_TGCA'G

Cuts at:      0    1547    1677 
   Size:       1547     130
 
SfcI C'TryA_G

Cuts at:      0    1543    1677 
   Size:       1543     134
 
SspI AAT'ATT

Cuts at:      0    1673    1677 
   Size:       1673       4
 
StuI AGG'CCT

Cuts at:      0     495     881    1677 
   Size:        495     386     796

  Fragments arranged by size: 

                 796     495     386
 
StyI C'CwwG_G

Cuts at:      0     869    1377    1677 
   Size:        869     508     300

  Fragments arranged by size: 

                 869     508     300
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1619    1677 
   Size:       1619      58
 
TatI w'GTAC_w

Cuts at:      0     150     640     740    1677 
   Size:        150     490     100     937

  Fragments arranged by size: 

                 937     490     150     100
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     578     610    1677 
   Size:        578      32    1067

  Fragments arranged by size: 

                1067     578      32
 
XbaI T'CTAG_A

Cuts at:      0     141    1326    1677 
   Size:        141    1185     351

  Fragments arranged by size: 

                1185     351     141
 
XmnI GAAnn'nnTTC

Cuts at:      0     452    1138    1677 
   Size:        452     686     539

  Fragments arranged by size: 

                 686     539     452
 

 Enzymes that do cut:

   Acc65I     AclI     AcuI   AflIII     AlfI    AlwNI     ApoI     AseI
     BanI    BciVI     BclI    BglII Bme1580I    BmgBI     BmrI     BpmI
    BsaXI    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrDI
    BsrGI    BstBI    BstYI     BtgI     BtsI     ClaI    CspCI     DraI
     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    Hin4I
     KpnI     MmeI     MscI     MslI     NcoI     NdeI     NspI     PciI
    PflMI     PfoI    PpuMI     PstI     SfcI     SspI     StuI     StyI
    TaqII     TatI     TstI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AfeI    AflII     AgeI     AhdI     AleI
     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI
    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI    BfrBI     BglI
     BlpI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspMI
    BsrBI    BsrFI   BssHII    BssSI   BstAPI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     MfeI     MluI   MspA1I     NaeI     NarI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SwaI  Tth111I     XcmI     XhoI     XmaI     ZraI
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