Restriction map for pF1KB3205
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BamHI       BbeI        
BbrPI       BfrI        BglII       BlnI        BsaBI       
BsaHI       BsiEI       BsiWI       BsmI        BsoBI       
Bsp1407I    BspDI       BspMI       BsrBI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstYI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DraIII      DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoT22I     FseI        FspI        
HgaI        Hin1I       HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MflI        
MluI        MunI        MvnI        NarI        NcoI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
XbaI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AcsI          797  GAATTC               [GA]^AATT[CT]
AflIII        115  ACATGT               A^C[GA][CT]GT
AlwI         1353  GATCC                GGATC(4/5)
ApaI          983  GGGCCC               GGGCC^C
ApoI          797  GAATTC               [GA]^AATT[CT]
BanI         1221  GGTGCC               G^G[CT][GA]CC
BbvCI        1213  CCTCAGC              CCTCAGC(-5/-2)
BcgI         1185  GCAAGTGTGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         422  GTATCC               GTATCC(6/5)
BsaAI         680  CACGTA               [CT]AC^GT[GA]
BsaI         1426  GAGACC               GGTCTC(1/5)
BsgI         1113  CTGCAC               GTGCAG(16/14)
BspHI         653  TCATGA               T^CATGA
BspLU11I      115  ACATGT               A^CATGT
BsrFI        1601  GCCGGC               [GA]^CCGG[CT]
BstAPI        342  GCAGCAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        1356  CCAGAACTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          690  GCGC                 GCG^C
Cfr10I       1601  GCCGGC               [GA]^CCGG[CT]
DrdI          631  GACTCCTGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI         797  GAATTC               G^AATTC
EcoRV         103  GATATC               GAT^ATC
HaeII         689  GGCGCT               [GA]GCGC^[CT]
HhaI          690  GCGC                 GCG^C
HinP1I        690  GCGC                 G^CGC
HincII        430  GTCGAC               GT[CT]^[GA]AC
HindII        430  GTCGAC               GT[CT]^[GA]AC
MaeII         681  ACGT                 A^CGT
MseI          659  TTAA                 T^TAA
NaeI         1601  GCCGGC               GCC^GGC
NdeI         1482  CATATG               CA^TATG
NgoMIV       1601  GCCGGC               G^CCGGC
NheI         1135  GCTAGC               G^CTAGC
NspI          115  ACATGT               [GA]CATG^[CT]
PflMI        1225  CCAGGGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         123  GGGACCC              [GA]G^G[AT]CC[CT]
PshAI         125  GACCCAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         1507  CTGCAG               CTGCA^G
PvuII        1216  CAGCTG               CAG^CTG
RcaI          653  TCATGA               T^CATGA
SalI          430  GTCGAC               G^TCGAC
Tru9I         659  TTAA                 T^TAA
Van91I       1225  CCAGGGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1283  CCATGACTGGTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccI          430  GTCGAC               GT^[AC][GT]AC
             1382  GTCTAC              
AccIII         52  TCCGGA               T^CCGGA
              944  TCCGGA              
BalI         1303  TGGCCA               TGG^CCA
             1626  TGGCCA              
BanII         983  GGGCCC               G[GA]GC[CT]^C
             1613  GGGCTC              
BbsI          628  GAAGAC               GAAGAC(2/6)
              948  GAAGAC              
BclI          365  TGATCA               T^GATCA
              398  TGATCA              
BcnI          986  CCCGG                CC^[GC]GG
             1128  CCGGG               
BglI          976  GCCTGTGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1601  GCCGGCAAGGC         
BpuAI         628  GAAGAC               GAAGAC(2/6)
              948  GAAGAC              
BsaWI          52  TCCGGA               [AT]^CCGG[AT]
              944  TCCGGA              
BseAI          52  TCCGGA               T^CCGGA
              944  TCCGGA              
BsmBI        1447  GAGACG               CGTCTC(1/5)
             1531  GAGACG              
BsmFI         123  GGGAC                GGGAC(10/14)
              280  GGGAC               
BspEI          52  TCCGGA               T^CCGGA
              944  TCCGGA              
BssSI         776  CACGAG               CACGAG(-5/-1)
               22  CTCGTG              
EarI          265  GAAGAG               CTCTTC(1/4)
              814  GAAGAG              
EcoT14I      1106  CCAAGG               C^C[AT][AT]GG
             1277  CCAAGG              
FauI          915  GCGGG                CCCGC(4/6)
             1610  GCGGG               
FbaI          365  TGATCA               T^GATCA
              398  TGATCA              
Ksp632I       265  GAAGAG               CTCTTC(1/4)
              814  GAAGAG              
MaeIII        440  GTGAC                ^GT[ACGT]AC
              670  GTGAC               
MluNI        1303  TGGCCA               TGG^CCA
             1626  TGGCCA              
MroI           52  TCCGGA               T^CCGGA
              944  TCCGGA              
MscI         1303  TGGCCA               TGG^CCA
             1626  TGGCCA              
MslI          168  CATTGTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1182  CATGCAAGTG          
MspA1I        224  CAGCGG               C[AC]G^C[GT]G
             1216  CAGCTG              
NciI          986  CCCGG                CC^[GC]GG
             1128  CCGGG               
SfcI          320  CTGTAG               C^T[GA][CT]AG
             1507  CTGCAG              
StuI         1041  AGGCCT               AGG^CCT
             1109  AGGCCT              
StyI         1106  CCAAGG               C^C[AT][AT]GG
             1277  CCAAGG              
Tsp45I        440  GTGAC                ^GT[GC]AC
              670  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AciI        AfaI        AluI        AspHI       AvaII       
BbvI        BfaI        BlpI        BmyI        BpmI        
Bpu1102I    BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    BsrDI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       CelII       
Cfr13I      DdeI        DpnII       DraII       EaeI        
EcoO109I    EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
MaeI        MboI        MboII       MnlI        MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       TaqI        TfiI        TseI        Tsp509I     
TspRI       TthHB8I     
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