Restriction map for pF1KB3205
 (Linear) (Six-base) MAPSORT of: KIBB3205.seq  Check: 2618  from: 1  to: 1635



KIBB3205.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AccI GT'mk_AC

Cuts at:      0     431    1383    1635 
   Size:        431     952     252

  Fragments arranged by size: 

                 952     431     252
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     874    1635 
   Size:        874     761
 
AflIII A'CryG_T

Cuts at:      0     115    1635 
   Size:        115    1520
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     733    1635 
   Size:        733     902
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      47      79    1154    1186    1635 
   Size:         47      32    1075      32     449

  Fragments arranged by size: 

                1075     449      47      32      32
 
ApaI G_GGCC'C

Cuts at:      0     987    1635 
   Size:        987     648
 
ApoI r'AATT_y

Cuts at:      0     797    1635 
   Size:        797     838
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1445    1478    1635 
   Size:       1445      33     157

  Fragments arranged by size: 

                1445     157      33
 
BanI G'GyrC_C

Cuts at:      0    1221    1635 
   Size:       1221     414
 
BanII G_rGCy'C

Cuts at:      0     987    1617    1635 
   Size:        987     630      18

  Fragments arranged by size: 

                 987     630      18
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     635     955    1635 
   Size:        635     320     680

  Fragments arranged by size: 

                 680     635     320
 
BbvCI CC'TCA_GC

Cuts at:      0    1214    1635 
   Size:       1214     421
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1174    1208    1635 
   Size:       1174      34     427

  Fragments arranged by size: 

                1174     427      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     433    1635 
   Size:        433    1202
 
BclI T'GATC_A

Cuts at:      0     365     398    1635 
   Size:        365      33    1237

  Fragments arranged by size: 

                1237     365      33
 
BglI GCCn_nnn'nGGC

Cuts at:      0     982    1607    1635 
   Size:        982     625      28

  Fragments arranged by size: 

                 982     625      28
 
BlpI GC'TnA_GC

Cuts at:      0      28     326     899    1635 
   Size:         28     298     573     736

  Fragments arranged by size: 

                 736     573     298      28
 
Bme1580I G_kGCm'C

Cuts at:      0     987    1635 
   Size:        987     648
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     421    1486    1635 
   Size:        421    1065     149

  Fragments arranged by size: 

                1065     421     149
 
BmtI G_CTAG'C

Cuts at:      0    1139    1635 
   Size:       1139     496
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1167    1635 
   Size:       1167     468
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358     635     787    1156    1635 
   Size:        358     277     152     369     479

  Fragments arranged by size: 

                 479     369     358     277     152
 
Bpu10I CC'TnA_GC

Cuts at:      0    1214    1635 
   Size:       1214     421
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1420    1635 
   Size:       1420     215
 
BsaAI yAC'GTr

Cuts at:      0     682    1635 
   Size:        682     953
 
BsaWI w'CCGG_w

Cuts at:      0      52     944    1635 
   Size:         52     892     691

  Fragments arranged by size: 

                 892     691      52
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     155     185     802     832    1154    1184    1635 
   Size:        155      30     617      30     322      30     451

  Fragments arranged by size: 

                 617     451     322     155      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     571     796    1114    1635 
   Size:        571     225     318     521

  Fragments arranged by size: 

                 571     521     318     225
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1098    1635 
   Size:       1098     537
 
BsiHKAI G_wGCw'C

Cuts at:      0      23      29     332    1635 
   Size:         23       6     303    1303

  Fragments arranged by size: 

                1303     303      23       6
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1441    1525    1635 
   Size:       1441      84     110

  Fragments arranged by size: 

                1441     110      84
 
Bsp1286I G_dGCh'C

Cuts at:      0      23      29     332     987    1617    1635 
   Size:         23       6     303     655     630      18

  Fragments arranged by size: 

                 655     630     303      23      18       6
 
BspEI T'CCGG_A

Cuts at:      0      52     944    1635 
   Size:         52     892     691

  Fragments arranged by size: 

                 892     691      52
 
BspHI T'CATG_A

Cuts at:      0     653    1635 
   Size:        653     982
 
BsrDI GCAATG_nn'

Cuts at:      0     195     521     965    1203    1635 
   Size:        195     326     444     238     432

  Fragments arranged by size: 

                 444     432     326     238     195
 
BsrFI r'CCGG_y

Cuts at:      0    1601    1635 
   Size:       1601      34
 
BssSI C'ACGA_G

Cuts at:      0      22     776    1635 
   Size:         22     754     859

  Fragments arranged by size: 

                 859     754      22
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     348    1635 
   Size:        348    1287
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1363    1635 
   Size:       1363     272
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1604    1635 
   Size:       1604      31
 
BtsI GCAGTG_nn'

Cuts at:      0    1532    1635 
   Size:       1532     103
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     637    1635 
   Size:        637     998
 
EaeI y'GGCC_r

Cuts at:      0     227    1303    1626    1635 
   Size:        227    1076     323       9

  Fragments arranged by size: 

                1076     323     227       9
 
EarI CTCTTCn'nnn_

Cuts at:      0     260     809    1635 
   Size:        260     549     826

  Fragments arranged by size: 

                 826     549     260
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358     635     787     874    1156    1635 
   Size:        358     277     152      87     282     479

  Fragments arranged by size: 

                 479     358     282     277     152      87
 
EcoO109I rG'GnC_Cy

Cuts at:      0     124     983    1235    1326    1635 
   Size:        124     859     252      91     309

  Fragments arranged by size: 

                 859     309     252     124      91
 
EcoRI G'AATT_C

Cuts at:      0     797    1635 
   Size:        797     838
 
EcoRV GAT'ATC

Cuts at:      0     105    1635 
   Size:        105    1530
 
HaeII r_GCGC'y

Cuts at:      0     693    1635 
   Size:        693     942
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      26    1635 
   Size:         26    1609
 
HincII GTy'rAC

Cuts at:      0     432    1635 
   Size:        432    1203
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     252     547     873    1008    1028    1635 
   Size:        252     295     326     135      20     607

  Fragments arranged by size: 

                 607     326     295     252     135      20
 
MscI TGG'CCA

Cuts at:      0    1305    1628    1635 
   Size:       1305     323       7

  Fragments arranged by size: 

                1305     323       7
 
MslI CAynn'nnrTG

Cuts at:      0     172    1186    1635 
   Size:        172    1014     449

  Fragments arranged by size: 

                1014     449     172
 
MspA1I CmG'CkG

Cuts at:      0     226    1218    1635 
   Size:        226     992     417

  Fragments arranged by size: 

                 992     417     226
 
NaeI GCC'GGC

Cuts at:      0    1603    1635 
   Size:       1603      32
 
NdeI CA'TA_TG

Cuts at:      0    1483    1635 
   Size:       1483     152
 
NgoMIV G'CCGG_C

Cuts at:      0    1601    1635 
   Size:       1601      34
 
NheI G'CTAG_C

Cuts at:      0    1135    1635 
   Size:       1135     500
 
NspI r_CATG'y

Cuts at:      0     119    1635 
   Size:        119    1516
 
PciI A'CATG_T

Cuts at:      0     115    1635 
   Size:        115    1520
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1231    1635 
   Size:       1231     404
 
PfoI T'CCnGG_A

Cuts at:      0     335    1172    1205    1635 
   Size:        335     837      33     430

  Fragments arranged by size: 

                 837     430     335      33
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1154    1186    1635 
   Size:       1154      32     449

  Fragments arranged by size: 

                1154     449      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     124    1635 
   Size:        124    1511
 
PshAI GACnn'nnGTC

Cuts at:      0     129    1635 
   Size:        129    1506
 
PsiI TTA'TAA

Cuts at:      0    1517    1635 
   Size:       1517     118
 
PspOMI G'GGCC_C

Cuts at:      0     983    1635 
   Size:        983     652
 
PstI C_TGCA'G

Cuts at:      0    1511    1635 
   Size:       1511     124
 
PvuII CAG'CTG

Cuts at:      0    1218    1635 
   Size:       1218     417
 
SalI G'TCGA_C

Cuts at:      0     430    1635 
   Size:        430    1205
 
SanDI GG'GwC_CC

Cuts at:      0     124    1635 
   Size:        124    1511
 
SfcI C'TryA_G

Cuts at:      0     320    1507    1635 
   Size:        320    1187     128

  Fragments arranged by size: 

                1187     320     128
 
StuI AGG'CCT

Cuts at:      0    1043    1111    1635 
   Size:       1043      68     524

  Fragments arranged by size: 

                1043     524      68
 
StyI C'CwwG_G

Cuts at:      0    1106    1277    1635 
   Size:       1106     171     358

  Fragments arranged by size: 

                1106     358     171
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     368    1426    1635 
   Size:        368    1058     209

  Fragments arranged by size: 

                1058     368     209
 
TatI w'GTAC_w

Cuts at:      0     818    1635 
   Size:        818     817
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     155     187    1635 
   Size:        155      32    1448

  Fragments arranged by size: 

                1448     155      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1290    1635 
   Size:       1290     345
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AlfI     AloI     ApaI     ApoI     BaeI
     BanI    BanII     BbsI    BbvCI     BcgI    BciVI     BclI     BglI
     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BspHI    BsrDI    BsrFI    BssSI   BstAPI    BstXI    BtgZI
     BtsI     DrdI     EaeI     EarI  Eco57MI EcoO109I    EcoRI    EcoRV
    HaeII    Hin4I   HincII     MmeI     MscI     MslI   MspA1I     NaeI
     NdeI   NgoMIV     NheI     NspI     PciI    PflMI     PfoI     PpiI
    PpuMI    PshAI     PsiI   PspOMI     PstI    PvuII     SalI    SanDI
     SfcI     StuI     StyI    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI    AlwNI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
    BamHI     BbeI    BfrBI    BglII    BmgBI    BpuEI    BsaBI    BsaHI
    BseYI    BsiEI    BsiWI     BsmI    BspMI    BsrBI    BsrGI   BssHII
    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI
     DraI   DraIII     EagI     EciI  EcoICRI    EcoNI     FalI     FseI
     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NarI     NcoI     NotI     NruI     NsiI     PacI     PasI     PmeI
     PmlI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI
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