Restriction map for pF1KB3122
##################################
# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AvaI        AvaII       AviII       AvrII       
BamHI       BanII       BbeI        BbrPI       BbsI        
BbvI        BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsiYI       BslI        BsmFI       
BsmI        BsoBI       BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstF5I      
BstUI       BstYI       BstZ17I     Bsu36I      Cac8I       
CelII       Cfr10I      Cfr13I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoRV       EcoT14I     
EcoT22I     FbaI        Fnu4HI      FokI        FseI        
FspI        HapII       HgaI        Hin1I       HpaI        
HpaII       ItaI        KasI        KpnI        KspI        
MaeI        MaeII       MamI        MflI        MluI        
MroI        MspA1I      MspI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIII      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    StuI        StyI        SwaI        TaqI        
TseI        TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   60 enzymes) #
###################################

AccI          332  GTAGAC               GT^[AC][GT]AC
AciI          505  CCGC                 CCGC(-3/-1)
AcsI          118  GAATTC               [GA]^AATT[CT]
AfaI          125  GTAC                 GT^AC
AlwI          499  GATCC                GGATC(4/5)
AlwNI         341  CAGTGACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          118  GAATTC               [GA]^AATT[CT]
AspHI         183  GAGCAC               G[AT]GC[AT]^C
AspI          592  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          528  TGGCCA               TGG^CCA
BanI          630  GGTGCC               G^G[CT][GA]CC
BbvCI         367  CCTCAGC              CCTCAGC(-5/-2)
BmyI          183  GAGCAC               G[AGT]GC[ACT]^C
BsaJI         219  CCGAGG               C^C[ACGT][ACGT]GG
BseRI         546  GAGGAG               GAGGAG(10/8)
BsiHKAI       183  GAGCAC               G[AT]GC[AT]^C
BsmBI         474  CGTCTC               CGTCTC(1/5)
Bsp1286I      183  GAGCAC               G[AGT]GC[ACT]^C
Bsp1407I      124  TGTACA               T^GTACA
BspMI         539  ACCTGC               ACCTGC(4/8)
BsrGI         124  TGTACA               T^GTACA
BssKI         436  CCAGG                ^CC[ACGT]GG
BstEII        639  GGTCACC              G^GT[ACGT]ACC
BstNI         436  CCAGG                CC^[AT]GG
BstPI         639  GGTCACC              G^GT[ACGT]ACC
BstXI         620  CCATTCCTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII         499  GATC                 ^GATC
EaeI          528  TGGCCA               [CT]^GGCC[GA]
EarI          271  CTCTTC               CTCTTC(1/4)
EcoO65I       639  GGTCACC              G^GT[ACGT]ACC
EcoRI         118  GAATTC               G^AATTC
EcoRII        436  CCAGG                ^CC[AT]GG
FauI          504  CCCGC                CCCGC(4/6)
HaeII         461  GGCGCT               [GA]GCGC^[CT]
HincII        598  GTCAAC               GT[CT]^[GA]AC
HindII        598  GTCAAC               GT[CT]^[GA]AC
HindIII       146  AAGCTT               A^AGCTT
HinfI         469  GATTC                G^A[ACGT]TC
Ksp632I       271  CTCTTC               CTCTTC(1/4)
MboI          499  GATC                 ^GATC
MfeI          492  CAATTG               C^AATTG
MluNI         528  TGGCCA               TGG^CCA
MscI          528  TGGCCA               TGG^CCA
MslI          621  CATTCCTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          492  CAATTG               C^AATTG
MvaI          436  CCAGG                CC^[AT]GG
MwoI          139  GCAAGAGAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII         499  GATC                 ^GATC
NlaIV         630  GGTGCC               GG[ACGT]^[ACGT]CC
PflFI         592  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI          541  CTGCAG               CTGCA^G
RsaI          125  GTAC                 GT^AC
Sau3AI        499  GATC                 ^GATC
ScrFI         436  CCAGG                CC^[ACGT]GG
SfaNI         308  GATGC                GCATC(5/9)
SfcI          541  CTGCAG               C^T[GA][CT]AG
SspBI         124  TGTACA               T^GTACA
SspI          379  AATATT               AAT^ATT
TfiI          469  GATTC                G^A[AT]TC
Tth111I       592  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

AluI           60  AGCT                 AG^CT
              147  AGCT                
BpmI          434  CTCCAG               CTGGAG(16/14)
              303  CTGGAG              
BsmAI         432  GTCTC                GTCTC(1/5)
              475  GTCTC               
CfoI          199  GCGC                 GCG^C
              462  GCGC                
HhaI          199  GCGC                 GCG^C
              462  GCGC                
HinP1I        199  GCGC                 G^CGC
              462  GCGC                
HphI          386  TCACC                GGTGA(8/7)
              641  TCACC               


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

BsrI        DdeI        HaeIII      MaeIII      MboII       
MnlI        MseI        Tru9I       Tsp45I      Tsp509I     
TspRI       
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