Restriction map for
pF1KB3115
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# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflIII AgeI AhdI Alw44I
AlwI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BbvI BcgI BciVI BcnI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmAI BsmBI
BsmFI BsmI BspDI BspEI BspHI
BspLU11I BspMI BsrFI BssHII Bst1107I
BstAPI BstEII BstPI BstXI BstYI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI FauI
FseI FspI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MfeI MflI MluI MluNI MroI
MscI MslI MspI MunI NaeI
NarI NciI NdeI NgoMIV NotI
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SbfI
ScaI SfcI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI TseI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccII 862 CGCG CG^CG
AflII 370 CTTAAG C^TTAAG
AlwNI 346 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 991 CCTCAGG CC^T[ACGT]AGG
AvaI 136 CTCGGG C^[CT]CG[GA]G
BfaI 451 CTAG C^TAG
BfrI 370 CTTAAG C^TTAAG
BpmI 13 CTGGAG CTGGAG(16/14)
BsaBI 498 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 136 CTCGGG C^[CT]CG[GA]G
BsrBI 1162 GAGCGG CCGCTC(-3/-3)
BssSI 781 CACGAG CACGAG(-5/-1)
BstBI 220 TTCGAA TT^CGAA
BstUI 862 CGCG CG^CG
Bsu36I 991 CCTCAGG CC^T[ACGT]AGG
DrdI 1063 GACGGTATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 247 CTCTTC CTCTTC(1/4)
Eco81I 991 CCTCAGG CC^T[ACGT]AGG
EcoRV 502 GATATC GAT^ATC
EcoT22I 773 ATGCAT ATGCA^T
Fnu4HI 1164 GCGGC GC^[ACGT]GC
ItaI 1164 GCGGC GC^[ACGT]GC
Ksp632I 247 CTCTTC CTCTTC(1/4)
MaeI 451 CTAG C^TAG
MaeIII 548 GTTAC ^GT[ACGT]AC
MamI 498 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 409 CAGCTG C[AC]G^C[GT]G
MvnI 862 CGCG CG^CG
NheI 450 GCTAGC G^CTAGC
NlaIV 520 GGAGCC GG[ACGT]^[ACGT]CC
NruI 861 TCGCGA TCG^CGA
NsiI 773 ATGCAT ATGCA^T
NspI 423 ACATGC [GA]CATG^[CT]
NspV 220 TTCGAA TT^CGAA
PvuII 409 CAGCTG CAG^CTG
SacI 599 GAGCTC GAGCT^C
SapI 246 GCTCTTC GCTCTTC(1/4)
SexAI 830 ACCTGGT A^CC[AT]GGT
SfuI 220 TTCGAA TT^CGAA
SspI 916 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 643 GCGG CCGC(-3/-1)
1164 GCGG
AcyI 3 GGCGTC G[GA]^CG[CT]C
169 GGCGTC
AvaII 988 GGACC G^G[AT]CC
1179 GGACC
BclI 80 TGATCA T^GATCA
1128 TGATCA
BsaHI 3 GGCGTC G[GA]^CG[CT]C
169 GGCGTC
BsgI 1015 CTGCAC GTGCAG(16/14)
976 GTGCAG
BsiYI 349 CCCCTGCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
825 CCGAAACCTGG
BslI 349 CCCCTGCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
825 CCGAAACCTGG
Bsp1407I 150 TGTACA T^GTACA
1209 TGTACA
BsrDI 770 GCAATG GCAATG(2/0)
888 GCAATG
BsrGI 150 TGTACA T^GTACA
1209 TGTACA
Cfr13I 988 GGACC G^G[ACGT]CC
1179 GGACC
DsaI 638 CCATGG C^C[GA][CT]GG
1175 CCATGG
FbaI 80 TGATCA T^GATCA
1128 TGATCA
HgaI 4 GCGTC GACGC(5/10)
170 GCGTC
Hin1I 3 GGCGTC G[GA]^CG[CT]C
169 GGCGTC
MaeII 488 ACGT A^CGT
1010 ACGT
NcoI 638 CCATGG C^CATGG
1175 CCATGG
Sau96I 988 GGACC G^G[ACGT]CC
1179 GGACC
SmlI 50 CTCAAG C^T[CT][GA]AG
370 CTTAAG
SspBI 150 TGTACA T^GTACA
1209 TGTACA
TaqI 221 TCGA T^CGA
279 TCGA
Tsp509I 360 AATT ^AATT
741 AATT
TthHB8I 221 TCGA T^CGA
279 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AfaI AluI AspHI BanII BmyI
BsaJI BsiHKAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I DdeI DpnII
EcoRII EcoT14I FokI HinfI HphI
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII RsaI Sau3AI
ScrFI SfaNI StyI TfiI Tru9I
TspRI